Paenibacillus zeisoli
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A433XIC4|A0A433XIC4_9BACL FtsX-like permease family protein OS=Paenibacillus zeisoli OX=2496267 GN=EJP77_08305 PE=4 SV=1
MM1 pKa = 7.1 YY2 pKa = 10.04 VVCKK6 pKa = 10.56 DD7 pKa = 3.58 HH8 pKa = 7.48 VEE10 pKa = 3.96 LAIDD14 pKa = 3.47 MFVDD18 pKa = 3.92 EE19 pKa = 6.03 FEE21 pKa = 4.76 DD22 pKa = 4.47 APDD25 pKa = 3.53 IVDD28 pKa = 3.83 LKK30 pKa = 10.11 EE31 pKa = 4.31 TEE33 pKa = 4.15 FSDD36 pKa = 2.87 WDD38 pKa = 3.93 PPVKK42 pKa = 10.44 CSQCDD47 pKa = 3.3 QAAVFLVVV55 pKa = 3.66
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 1.863
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A433XI74|A0A433XI74_9BACL Uncharacterized protein OS=Paenibacillus zeisoli OX=2496267 GN=EJP77_06455 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 8.7 PTFKK6 pKa = 10.72 PNVSKK11 pKa = 10.78 RR12 pKa = 11.84 SKK14 pKa = 9.04 VHH16 pKa = 5.83 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.067
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.676
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.398
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4013
0
4013
1259114
11
3386
313.8
34.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.041 ± 0.04
0.707 ± 0.012
5.063 ± 0.03
6.792 ± 0.05
3.955 ± 0.027
7.573 ± 0.04
2.132 ± 0.022
6.866 ± 0.032
5.489 ± 0.04
10.163 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.02
3.793 ± 0.03
4.017 ± 0.025
3.931 ± 0.025
4.967 ± 0.035
6.613 ± 0.035
5.469 ± 0.042
7.105 ± 0.03
1.179 ± 0.015
3.422 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here