Streptococcus phage Javan169
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A5T0|A0A4D6A5T0_9CAUD Exo-glucosaminidase OS=Streptococcus phage Javan169 OX=2548018 GN=Javan169_0007 PE=4 SV=1
MM1 pKa = 7.65 RR2 pKa = 11.84 KK3 pKa = 9.49 EE4 pKa = 3.74 EE5 pKa = 4.71 LIEE8 pKa = 4.52 EE9 pKa = 4.4 IEE11 pKa = 5.17 DD12 pKa = 4.07 EE13 pKa = 4.69 INDD16 pKa = 3.62 IDD18 pKa = 5.75 LLIDD22 pKa = 5.09 DD23 pKa = 4.74 YY24 pKa = 11.66 QCEE27 pKa = 4.1 IDD29 pKa = 3.79 EE30 pKa = 4.74 LEE32 pKa = 4.4 RR33 pKa = 11.84 DD34 pKa = 3.55 VDD36 pKa = 3.88 EE37 pKa = 5.68 LDD39 pKa = 3.23 TRR41 pKa = 11.84 KK42 pKa = 10.72 DD43 pKa = 3.02 ILVRR47 pKa = 11.84 KK48 pKa = 9.89 LEE50 pKa = 4.12 SLLEE54 pKa = 4.27 SVEE57 pKa = 4.24 EE58 pKa = 4.15
Molecular weight: 7.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.308
IPC2_protein 3.846
IPC_protein 3.783
Toseland 3.592
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.503
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.024
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.897
Patrickios 3.694
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A4D6A1R7|A0A4D6A1R7_9CAUD Portal protein OS=Streptococcus phage Javan169 OX=2548018 GN=Javan169_0005 PE=4 SV=1
MM1 pKa = 7.36 TKK3 pKa = 7.85 QHH5 pKa = 6.91 RR6 pKa = 11.84 EE7 pKa = 3.47 TLIWYY12 pKa = 7.66 RR13 pKa = 11.84 ASHH16 pKa = 5.37 QEE18 pKa = 4.01 RR19 pKa = 11.84 EE20 pKa = 4.04 KK21 pKa = 11.28 LLDD24 pKa = 3.94 FGLVDD29 pKa = 3.68 KK30 pKa = 10.91 SQYY33 pKa = 8.62 VTLLRR38 pKa = 11.84 QLRR41 pKa = 11.84 KK42 pKa = 9.91 KK43 pKa = 10.48 YY44 pKa = 10.73 AII46 pKa = 4.1
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.164
IPC2_protein 9.487
IPC_protein 9.663
Toseland 10.145
ProMoST 9.809
Dawson 10.335
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.76
Grimsley 10.394
Solomon 10.379
Lehninger 10.35
Nozaki 10.101
DTASelect 9.999
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.233
Patrickios 10.54
IPC_peptide 10.379
IPC2_peptide 8.565
IPC2.peptide.svr19 8.565
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
13088
36
1312
187.0
21.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.533 ± 0.645
0.634 ± 0.109
6.907 ± 0.329
7.61 ± 0.359
3.82 ± 0.21
6.189 ± 0.38
1.222 ± 0.112
6.961 ± 0.333
9.23 ± 0.327
8.626 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.19
5.616 ± 0.216
2.896 ± 0.23
4.195 ± 0.195
4.233 ± 0.212
5.899 ± 0.318
5.876 ± 0.253
5.983 ± 0.22
1.108 ± 0.114
3.958 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here