Pantoea phage LIMEzero

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Waewaevirus; Pantoea virus Limezero

Average proteome isoelectric point is 5.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4N9R6|F4N9R6_9CAUD Uncharacterized protein OS=Pantoea phage LIMEzero OX=943335 PE=4 SV=1
MM1 pKa = 6.45TTNIEE6 pKa = 4.34YY7 pKa = 10.11IIKK10 pKa = 10.33QYY12 pKa = 10.9VDD14 pKa = 3.49PVDD17 pKa = 4.18GEE19 pKa = 4.54TTFAAFTPDD28 pKa = 3.35LQFVAIAPTKK38 pKa = 9.75WDD40 pKa = 4.07LDD42 pKa = 3.42QCIAKK47 pKa = 10.28GLVDD51 pKa = 4.13EE52 pKa = 5.18PVAQILEE59 pKa = 4.34NEE61 pKa = 4.28DD62 pKa = 3.42YY63 pKa = 10.87EE64 pKa = 4.77YY65 pKa = 11.46DD66 pKa = 3.92DD67 pKa = 3.96YY68 pKa = 11.23RR69 pKa = 11.84TSLRR73 pKa = 11.84AALAAPAVVV82 pKa = 3.55

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4N9T0|F4N9T0_9CAUD Uncharacterized protein OS=Pantoea phage LIMEzero OX=943335 PE=4 SV=1
MM1 pKa = 8.27VDD3 pKa = 3.43MKK5 pKa = 11.5LMVVDD10 pKa = 4.8HH11 pKa = 6.79DD12 pKa = 4.34HH13 pKa = 5.84STGEE17 pKa = 3.8IRR19 pKa = 11.84GVLCRR24 pKa = 11.84WCNAQLGKK32 pKa = 9.64MEE34 pKa = 4.36NAATRR39 pKa = 11.84AKK41 pKa = 10.63RR42 pKa = 11.84NLTPEE47 pKa = 3.58QWLDD51 pKa = 3.02NAFQHH56 pKa = 6.54RR57 pKa = 11.84KK58 pKa = 8.46AHH60 pKa = 6.01TGLMYY65 pKa = 7.7PTHH68 pKa = 6.28LTAAEE73 pKa = 4.28KK74 pKa = 10.02VAKK77 pKa = 10.17SAAKK81 pKa = 10.06RR82 pKa = 11.84KK83 pKa = 9.52ASAMAKK89 pKa = 9.99AAAKK93 pKa = 10.3LKK95 pKa = 10.71AKK97 pKa = 9.64QQ98 pKa = 3.15

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13316

22

1031

233.6

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.957 ± 0.489

0.999 ± 0.167

6.053 ± 0.235

5.227 ± 0.333

3.312 ± 0.15

7.803 ± 0.324

1.772 ± 0.168

4.649 ± 0.225

4.055 ± 0.287

7.96 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.302

4.303 ± 0.3

4.108 ± 0.236

5.422 ± 0.807

5.197 ± 0.367

6.511 ± 0.393

6.706 ± 0.456

7.179 ± 0.309

1.299 ± 0.131

3.485 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski