Boudabousia marimammalium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Boudabousia

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5PSA7|A0A1Q5PSA7_9ACTO GMP synthase [glutamine-hydrolyzing] OS=Boudabousia marimammalium OX=156892 GN=guaA PE=3 SV=1
MM1 pKa = 7.52GATNEE6 pKa = 4.05VTILDD11 pKa = 3.99PAEE14 pKa = 4.67SYY16 pKa = 11.7DD17 pKa = 3.36NGTFYY22 pKa = 11.32VAIQALAFLCNSPYY36 pKa = 10.86GEE38 pKa = 4.52PEE40 pKa = 4.09VKK42 pKa = 10.0PDD44 pKa = 3.62LAEE47 pKa = 4.18SDD49 pKa = 3.84EE50 pKa = 4.61FNAPTEE56 pKa = 4.23FTVKK60 pKa = 10.17LRR62 pKa = 11.84EE63 pKa = 4.05GLKK66 pKa = 9.16FTNGQDD72 pKa = 4.64LSLLLYY78 pKa = 10.55NLEE81 pKa = 4.34SVDD84 pKa = 4.22AVDD87 pKa = 4.7DD88 pKa = 3.98NTVVFHH94 pKa = 6.8LL95 pKa = 4.55

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5PT60|A0A1Q5PT60_9ACTO Nitrate reductase OS=Boudabousia marimammalium OX=156892 GN=BM477_01445 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84AKK14 pKa = 8.68THH16 pKa = 5.34GFRR19 pKa = 11.84KK20 pKa = 9.94RR21 pKa = 11.84MSTRR25 pKa = 11.84AGRR28 pKa = 11.84AILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.61GRR39 pKa = 11.84AEE41 pKa = 3.84LSAA44 pKa = 4.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1518

0

1518

537412

44

3064

354.0

38.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.027 ± 0.074

0.69 ± 0.017

5.44 ± 0.047

6.407 ± 0.058

3.338 ± 0.038

7.842 ± 0.053

1.993 ± 0.026

5.279 ± 0.046

3.75 ± 0.051

10.01 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.028

2.985 ± 0.039

4.97 ± 0.042

3.881 ± 0.046

5.829 ± 0.064

6.367 ± 0.05

5.962 ± 0.052

8.052 ± 0.051

1.435 ± 0.029

2.478 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski