Mint virus X
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5G7H3|Q5G7H3_9VIRU ORF1 protein OS=Mint virus X OX=301865 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 4.7 SYY4 pKa = 11.1 RR5 pKa = 11.84 AEE7 pKa = 3.97 LASAFTRR14 pKa = 11.84 TSLPLSKK21 pKa = 10.08 PIVVHH26 pKa = 5.75 TVAGAGKK33 pKa = 6.94 TTFIRR38 pKa = 11.84 RR39 pKa = 11.84 LIRR42 pKa = 11.84 HH43 pKa = 6.19 APFPTAITGGTPDD56 pKa = 4.29 PPHH59 pKa = 7.31 ISGQRR64 pKa = 11.84 ITAPPGPANIVDD76 pKa = 4.82 EE77 pKa = 4.62 YY78 pKa = 11.01 PLVDD82 pKa = 3.17 WAGADD87 pKa = 3.93 VIFADD92 pKa = 4.37 PLQHH96 pKa = 7.37 RR97 pKa = 11.84 GPTLPAHH104 pKa = 5.12 YY105 pKa = 8.91 TSSITHH111 pKa = 6.35 RR112 pKa = 11.84 FGRR115 pKa = 11.84 ATCEE119 pKa = 3.88 LLSKK123 pKa = 10.7 FGITAEE129 pKa = 4.21 SNKK132 pKa = 9.72 EE133 pKa = 3.82 DD134 pKa = 3.35 EE135 pKa = 5.39 VFFGWAFADD144 pKa = 3.97 DD145 pKa = 4.09 PEE147 pKa = 5.07 GAVICLDD154 pKa = 4.39 AEE156 pKa = 4.33 AQSLASWNGLEE167 pKa = 4.41 HH168 pKa = 7.19 LKK170 pKa = 10.58 PCEE173 pKa = 3.76 ALGATFPVVTVISGTPLEE191 pKa = 4.5 EE192 pKa = 4.94 ADD194 pKa = 4.07 AVDD197 pKa = 4.95 RR198 pKa = 11.84 YY199 pKa = 10.3 IALTRR204 pKa = 11.84 HH205 pKa = 4.8 TRR207 pKa = 11.84 LLRR210 pKa = 11.84 ILLL213 pKa = 4.1
Molecular weight: 23.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.566
IPC2_protein 5.461
IPC_protein 5.474
Toseland 5.881
ProMoST 5.792
Dawson 5.69
Bjellqvist 5.715
Wikipedia 5.69
Rodwell 5.664
Grimsley 6.046
Solomon 5.69
Lehninger 5.677
Nozaki 5.944
DTASelect 6.135
Thurlkill 6.109
EMBOSS 6.071
Sillero 6.046
Patrickios 4.164
IPC_peptide 5.715
IPC2_peptide 6.059
IPC2.peptide.svr19 6.009
Protein with the highest isoelectric point:
>tr|Q5G7H2|Q5G7H2_9VIRU TGB 1 OS=Mint virus X OX=301865 PE=4 SV=1
MM1 pKa = 7.71 PLAPPPDD8 pKa = 3.66 YY9 pKa = 11.12 SKK11 pKa = 11.5 AVLAVAVGLAAALLVHH27 pKa = 6.09 QLTRR31 pKa = 11.84 STLPHH36 pKa = 6.48 SGDD39 pKa = 4.36 NIHH42 pKa = 6.58 SLPFGGSYY50 pKa = 11.07 VDD52 pKa = 3.39 GTKK55 pKa = 10.12 RR56 pKa = 11.84 IHH58 pKa = 6.63 FGGPSRR64 pKa = 11.84 GSSGHH69 pKa = 6.26 LWLPALIVAILPGIIWLGRR88 pKa = 11.84 PRR90 pKa = 11.84 AAHH93 pKa = 5.53 VCRR96 pKa = 11.84 CPLCAAARR104 pKa = 11.84 PPP106 pKa = 3.45
Molecular weight: 11.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.399
IPC_protein 9.984
Toseland 10.014
ProMoST 9.867
Dawson 10.262
Bjellqvist 10.072
Wikipedia 10.482
Rodwell 10.409
Grimsley 10.335
Solomon 10.35
Lehninger 10.321
Nozaki 10.204
DTASelect 10.014
Thurlkill 10.116
EMBOSS 10.438
Sillero 10.233
Patrickios 10.248
IPC_peptide 10.35
IPC2_peptide 9.531
IPC2.peptide.svr19 8.223
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1954
95
1312
390.8
43.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.798 ± 2.794
1.791 ± 0.378
5.578 ± 0.842
4.964 ± 1.213
5.015 ± 0.529
5.629 ± 1.427
3.992 ± 0.413
4.759 ± 0.562
4.094 ± 1.031
10.287 ± 0.732
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.586 ± 0.441
2.508 ± 0.631
7.216 ± 0.99
3.531 ± 1.114
5.681 ± 0.217
6.397 ± 0.559
7.062 ± 0.754
5.322 ± 0.328
1.331 ± 0.095
2.456 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here