Gordonia phage BaxterFox
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142KCP9|A0A142KCP9_9CAUD Uncharacterized protein OS=Gordonia phage BaxterFox OX=1821549 GN=72 PE=4 SV=1
MM1 pKa = 6.93 MACEE5 pKa = 5.33 QEE7 pKa = 5.2 LCVNWDD13 pKa = 4.23 GIGCPCAYY21 pKa = 10.58 LDD23 pKa = 3.58 TDD25 pKa = 3.39 EE26 pKa = 5.74 DD27 pKa = 3.94 EE28 pKa = 5.25 RR29 pKa = 11.84 PVVCGYY35 pKa = 10.25 CQTLLTVCAEE45 pKa = 4.17 RR46 pKa = 11.84 LCCPNCTHH54 pKa = 7.93 PYY56 pKa = 9.34 PGGPNGTGGDD66 pKa = 3.48 RR67 pKa = 3.65
Molecular weight: 7.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.029
IPC2_protein 4.113
IPC_protein 3.961
Toseland 3.783
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.706
Solomon 3.923
Lehninger 3.872
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.075
Patrickios 0.006
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|A0A1L7JNR4|A0A1L7JNR4_9CAUD Uncharacterized protein OS=Gordonia phage BaxterFox OX=1821549 GN=86 PE=4 SV=1
MM1 pKa = 7.25 PTAKK5 pKa = 9.64 QRR7 pKa = 11.84 NTTQRR12 pKa = 11.84 GLGWSHH18 pKa = 5.24 QKK20 pKa = 9.88 QRR22 pKa = 11.84 VRR24 pKa = 11.84 LLGKK28 pKa = 8.53 HH29 pKa = 6.03 TDD31 pKa = 3.51 TTPCWWCGEE40 pKa = 3.85 PMYY43 pKa = 10.47 RR44 pKa = 11.84 DD45 pKa = 3.77 PKK47 pKa = 10.58 RR48 pKa = 11.84 NPDD51 pKa = 3.58 YY52 pKa = 11.28 DD53 pKa = 3.84 PASNDD58 pKa = 3.67 PASGALAADD67 pKa = 3.98 HH68 pKa = 6.03 TRR70 pKa = 11.84 SRR72 pKa = 11.84 ATGGTVADD80 pKa = 4.41 RR81 pKa = 11.84 LLHH84 pKa = 5.56 GLCNKK89 pKa = 9.44 QRR91 pKa = 11.84 GDD93 pKa = 3.34 GSRR96 pKa = 11.84 DD97 pKa = 3.25 HH98 pKa = 6.57 QRR100 pKa = 11.84 PALLKK105 pKa = 10.54 RR106 pKa = 11.84 RR107 pKa = 11.84 GGHH110 pKa = 6.49 PANSLNWGG118 pKa = 3.56
Molecular weight: 13.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.502
IPC_protein 10.116
Toseland 10.628
ProMoST 10.277
Dawson 10.716
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.935
Grimsley 10.745
Solomon 10.833
Lehninger 10.804
Nozaki 10.643
DTASelect 10.394
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.643
Patrickios 10.701
IPC_peptide 10.833
IPC2_peptide 9.56
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
17391
41
2139
202.2
22.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.276 ± 0.634
0.845 ± 0.151
7.073 ± 0.386
6.015 ± 0.293
2.565 ± 0.163
8.257 ± 0.349
2.11 ± 0.232
4.445 ± 0.176
3.358 ± 0.19
7.377 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.076 ± 0.179
3.042 ± 0.204
5.566 ± 0.246
3.6 ± 0.259
7.613 ± 0.433
5.474 ± 0.205
7.09 ± 0.333
7.188 ± 0.239
2.018 ± 0.167
2.013 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here