Subdoligranulum sp. APC924/74

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Subdoligranulum; unclassified Subdoligranulum

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367FT45|A0A367FT45_9FIRM Uncharacterized protein OS=Subdoligranulum sp. APC924/74 OX=2086273 GN=C4885_16080 PE=4 SV=1
MM1 pKa = 6.92KK2 pKa = 10.08QNRR5 pKa = 11.84VISGAVAALVLAALLAGCGSTATGASSADD34 pKa = 3.43STPAATEE41 pKa = 3.96AASSEE46 pKa = 4.1ATAPTAEE53 pKa = 4.43PQTEE57 pKa = 3.9AALPDD62 pKa = 3.72GVYY65 pKa = 9.22TAEE68 pKa = 4.33FEE70 pKa = 4.52TDD72 pKa = 2.72SSMVHH77 pKa = 6.58ANEE80 pKa = 4.13ACDD83 pKa = 3.83GKK85 pKa = 10.51GTLTVADD92 pKa = 4.13GKK94 pKa = 8.53MTFHH98 pKa = 7.15VSLASKK104 pKa = 10.51KK105 pKa = 9.96IVNLYY110 pKa = 10.63LGTAEE115 pKa = 4.95DD116 pKa = 4.44APSHH120 pKa = 5.79EE121 pKa = 4.95SDD123 pKa = 2.86WLQPTTDD130 pKa = 3.25TVTYY134 pKa = 10.95SDD136 pKa = 3.66GTSDD140 pKa = 3.21EE141 pKa = 4.6VYY143 pKa = 10.71GYY145 pKa = 9.77DD146 pKa = 3.69IPLDD150 pKa = 3.87AVDD153 pKa = 4.82TDD155 pKa = 3.91FQLAILGTKK164 pKa = 7.28GTWYY168 pKa = 10.34DD169 pKa = 3.19HH170 pKa = 5.57TVSVRR175 pKa = 11.84NAEE178 pKa = 4.3PKK180 pKa = 10.17TEE182 pKa = 4.07DD183 pKa = 3.4AAAEE187 pKa = 4.16TAAVPADD194 pKa = 3.56GSYY197 pKa = 9.41TCAVTLEE204 pKa = 4.62GGSGRR209 pKa = 11.84ATVEE213 pKa = 3.97SPAALTVADD222 pKa = 3.92GTMTAVIVWSSPNYY236 pKa = 10.75DD237 pKa = 3.28YY238 pKa = 11.29MLIDD242 pKa = 3.7GEE244 pKa = 5.1KK245 pKa = 9.66YY246 pKa = 10.77LPTNTEE252 pKa = 3.98GNSTFEE258 pKa = 4.18IPVAALDD265 pKa = 3.54TALAVTADD273 pKa = 4.21TVAMSTPHH281 pKa = 6.76EE282 pKa = 4.09IDD284 pKa = 3.17YY285 pKa = 9.82TLTFDD290 pKa = 4.96SATLTPAEE298 pKa = 4.16

Molecular weight: 30.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367GIY2|A0A367GIY2_9FIRM Uncharacterized protein OS=Subdoligranulum sp. APC924/74 OX=2086273 GN=C4885_04330 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.52QTFQPKK8 pKa = 8.47KK9 pKa = 7.51RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.33GFLKK20 pKa = 10.64RR21 pKa = 11.84MASKK25 pKa = 10.75NGRR28 pKa = 11.84KK29 pKa = 9.16VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84KK40 pKa = 8.63NLTVV44 pKa = 3.1

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3080

0

3080

977135

37

2473

317.3

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.292 ± 0.063

1.768 ± 0.022

5.709 ± 0.035

6.155 ± 0.044

3.778 ± 0.033

7.394 ± 0.037

1.967 ± 0.018

5.493 ± 0.042

5.055 ± 0.04

9.597 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.024

3.752 ± 0.028

4.336 ± 0.028

3.848 ± 0.039

5.164 ± 0.041

5.162 ± 0.032

5.911 ± 0.041

6.903 ± 0.036

1.16 ± 0.019

3.747 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski