Caballeronia glathei
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7464 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A069Q019|A0A069Q019_9BURK LUD_dom domain-containing protein OS=Caballeronia glathei OX=60547 GN=BG61_02010 PE=4 SV=1
MM1 pKa = 7.44 FFFPPPPGAGGLAPWQTQTQPNTADD26 pKa = 3.76 LYY28 pKa = 10.48 TFLTTVAGVPTAALPSNSPYY48 pKa = 10.62 LAWALSFSEE57 pKa = 4.51 EE58 pKa = 3.77 NTLLVLRR65 pKa = 11.84 AVGQDD70 pKa = 3.45 YY71 pKa = 11.23 YY72 pKa = 11.63 CFAVYY77 pKa = 10.71 LLATSFLINWAPDD90 pKa = 3.25 QLGQTFFADD99 pKa = 5.97 LRR101 pKa = 11.84 NTWNISGFVGGIVQSTADD119 pKa = 3.34 QGTSEE124 pKa = 4.77 SMTMPDD130 pKa = 4.13 FLNGLTLGQLQALKK144 pKa = 10.83 DD145 pKa = 3.57 PFGRR149 pKa = 11.84 QWLAMSQEE157 pKa = 4.66 LGPIWGISS165 pKa = 3.18
Molecular weight: 17.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A069PHM9|A0A069PHM9_9BURK HTH gntR-type domain-containing protein OS=Caballeronia glathei OX=60547 GN=BG61_26915 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.74 TAGGRR28 pKa = 11.84 KK29 pKa = 9.04 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7464
0
7464
2290966
30
4431
306.9
33.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.877 ± 0.035
0.941 ± 0.009
5.398 ± 0.022
5.228 ± 0.025
3.771 ± 0.02
8.238 ± 0.027
2.296 ± 0.013
4.777 ± 0.019
3.099 ± 0.024
10.087 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.012
2.711 ± 0.022
5.098 ± 0.019
3.409 ± 0.018
7.132 ± 0.033
5.667 ± 0.017
5.322 ± 0.019
7.81 ± 0.023
1.351 ± 0.011
2.37 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here