Streptococcus phage Javan577

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AKQ9|A0A4D6AKQ9_9CAUD Terminase large subunit OS=Streptococcus phage Javan577 OX=2548269 GN=Javan577_0029 PE=4 SV=1
MM1 pKa = 7.33QSTGLFSTFSEE12 pKa = 4.69DD13 pKa = 4.38EE14 pKa = 4.15LFEE17 pKa = 6.72DD18 pKa = 6.13DD19 pKa = 4.96VIFWTYY25 pKa = 10.62FDD27 pKa = 4.26EE28 pKa = 6.49FEE30 pKa = 4.45DD31 pKa = 3.54TGKK34 pKa = 10.74ARR36 pKa = 11.84IVHH39 pKa = 7.19RR40 pKa = 11.84DD41 pKa = 3.64GCWKK45 pKa = 10.53LLDD48 pKa = 3.43IKK50 pKa = 10.73AGKK53 pKa = 8.71EE54 pKa = 3.89VWDD57 pKa = 3.96SLFDD61 pKa = 3.83CLEE64 pKa = 4.04NCTVFLSGNIYY75 pKa = 10.69EE76 pKa = 4.55NLEE79 pKa = 3.98LMEE82 pKa = 4.58GLNEE86 pKa = 4.14KK87 pKa = 10.34

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ALG6|A0A4D6ALG6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan577 OX=2548269 GN=Javan577_0011 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 9.87EE3 pKa = 3.74HH4 pKa = 6.99SIKK7 pKa = 10.6KK8 pKa = 9.92LLTGTITLLSVVTLVACSQSNKK30 pKa = 10.41GIIDD34 pKa = 3.68CRR36 pKa = 11.84IKK38 pKa = 10.68KK39 pKa = 10.47SPTLNFGRR47 pKa = 11.84SEE49 pKa = 3.91RR50 pKa = 11.84KK51 pKa = 9.59ANHH54 pKa = 5.48VWSEE58 pKa = 4.02TCFAVGLFTIPIYY71 pKa = 9.47QKK73 pKa = 10.83RR74 pKa = 11.84KK75 pKa = 7.48GKK77 pKa = 10.54SMAYY81 pKa = 8.96FRR83 pKa = 11.84KK84 pKa = 9.89RR85 pKa = 11.84EE86 pKa = 4.06NGWEE90 pKa = 3.66YY91 pKa = 10.51RR92 pKa = 11.84ISYY95 pKa = 8.24KK96 pKa = 10.93APDD99 pKa = 3.57GSYY102 pKa = 9.91KK103 pKa = 10.36QKK105 pKa = 10.58SKK107 pKa = 11.15SGFRR111 pKa = 11.84TKK113 pKa = 10.73SEE115 pKa = 3.93AVQAASQAEE124 pKa = 4.22IEE126 pKa = 4.31LSSGIVEE133 pKa = 4.78DD134 pKa = 4.43KK135 pKa = 10.91NISLAEE141 pKa = 3.97YY142 pKa = 9.35FEE144 pKa = 4.32KK145 pKa = 10.39WMEE148 pKa = 3.84IHH150 pKa = 6.86KK151 pKa = 10.21KK152 pKa = 9.28PHH154 pKa = 6.29VDD156 pKa = 2.98PEE158 pKa = 4.45TYY160 pKa = 9.81RR161 pKa = 11.84KK162 pKa = 10.35YY163 pKa = 10.95EE164 pKa = 4.03FNLKK168 pKa = 9.81IIKK171 pKa = 9.76EE172 pKa = 4.23YY173 pKa = 9.84YY174 pKa = 10.29QNTKK178 pKa = 10.02LSKK181 pKa = 9.44ITTTMHH187 pKa = 4.98QQVLNKK193 pKa = 10.34LADD196 pKa = 3.98RR197 pKa = 11.84YY198 pKa = 10.75VKK200 pKa = 10.0YY201 pKa = 9.04TVKK204 pKa = 10.61LFNSHH209 pKa = 4.62IRR211 pKa = 11.84AAIKK215 pKa = 10.31VALHH219 pKa = 5.79QGTLKK224 pKa = 10.66KK225 pKa = 10.75DD226 pKa = 3.45FTQLAKK232 pKa = 10.7VFSNVEE238 pKa = 4.17SKK240 pKa = 11.16DD241 pKa = 3.48EE242 pKa = 4.13SEE244 pKa = 6.13KK245 pKa = 10.63YY246 pKa = 10.66LEE248 pKa = 4.3LNGYY252 pKa = 9.96RR253 pKa = 11.84SLLLDD258 pKa = 3.17VRR260 pKa = 11.84QSINHH265 pKa = 5.28QSHH268 pKa = 6.24FFIYY272 pKa = 9.97TIGKK276 pKa = 6.89TGLRR280 pKa = 11.84FSEE283 pKa = 4.07ATGLSKK289 pKa = 10.0FTVDD293 pKa = 3.28RR294 pKa = 11.84QNMCLHH300 pKa = 5.75IRR302 pKa = 11.84KK303 pKa = 7.75TYY305 pKa = 10.1KK306 pKa = 10.6VYY308 pKa = 10.59GKK310 pKa = 10.35KK311 pKa = 10.25KK312 pKa = 9.78GWGPTKK318 pKa = 10.55NPQSEE323 pKa = 3.99RR324 pKa = 11.84DD325 pKa = 3.66VPIDD329 pKa = 3.87RR330 pKa = 11.84DD331 pKa = 3.21WLEE334 pKa = 4.8AYY336 pKa = 10.11DD337 pKa = 4.26EE338 pKa = 4.21YY339 pKa = 11.35MEE341 pKa = 5.0VGYY344 pKa = 10.28IDD346 pKa = 4.5NPEE349 pKa = 3.55KK350 pKa = 10.8RR351 pKa = 11.84LFFKK355 pKa = 11.0VSSTAANKK363 pKa = 9.92ALQKK367 pKa = 9.44KK368 pKa = 6.15TRR370 pKa = 11.84PTFTIHH376 pKa = 6.79GLRR379 pKa = 11.84HH380 pKa = 5.78TYY382 pKa = 10.53VSFLIYY388 pKa = 10.59NDD390 pKa = 3.95LDD392 pKa = 3.62VVTISKK398 pKa = 10.49LVGHH402 pKa = 7.25KK403 pKa = 9.02DD404 pKa = 2.99TTEE407 pKa = 3.89TLKK410 pKa = 10.27TYY412 pKa = 11.1AHH414 pKa = 6.9LFEE417 pKa = 5.37EE418 pKa = 4.69KK419 pKa = 9.88QAQNFDD425 pKa = 3.9KK426 pKa = 10.72IRR428 pKa = 11.84NLFEE432 pKa = 4.89KK433 pKa = 10.59FGADD437 pKa = 4.09LGQTT441 pKa = 3.65

Molecular weight: 51.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10784

43

1292

211.5

23.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.992 ± 0.508

0.566 ± 0.111

6.259 ± 0.309

7.826 ± 0.555

4.238 ± 0.307

6.667 ± 0.516

1.502 ± 0.177

6.714 ± 0.21

8.244 ± 0.558

7.845 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.184

5.666 ± 0.29

2.68 ± 0.171

3.746 ± 0.252

4.674 ± 0.239

6.417 ± 0.463

5.963 ± 0.47

6.25 ± 0.314

1.345 ± 0.139

3.82 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski