Amino acid dipepetide frequency for Pedobacter sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.736AlaAla: 5.736 ± 0.072	0.616AlaCys: 0.616 ± 0.02	4.061AlaAsp: 4.061 ± 0.046	4.251AlaGlu: 4.251 ± 0.054	3.437AlaPhe: 3.437 ± 0.042	5.031AlaGly: 5.031 ± 0.06	1.16AlaHis: 1.16 ± 0.025	5.741AlaIle: 5.741 ± 0.069	4.841AlaLys: 4.841 ± 0.06	6.863AlaLeu: 6.863 ± 0.064	1.782AlaMet: 1.782 ± 0.029	3.698AlaAsn: 3.698 ± 0.05	2.168AlaPro: 2.168 ± 0.034	2.678AlaGln: 2.678 ± 0.04	2.309AlaArg: 2.309 ± 0.037	4.599AlaSer: 4.599 ± 0.058	3.934AlaThr: 3.934 ± 0.058	4.631AlaVal: 4.631 ± 0.055	0.701AlaTrp: 0.701 ± 0.022	2.855AlaTyr: 2.855 ± 0.038	0.001AlaXaa: 0.001 ± 0.001
Cys	0.508CysAla: 0.508 ± 0.017	0.116CysCys: 0.116 ± 0.009	0.4CysAsp: 0.4 ± 0.016	0.375CysGlu: 0.375 ± 0.014	0.41CysPhe: 0.41 ± 0.014	0.616CysGly: 0.616 ± 0.018	0.173CysHis: 0.173 ± 0.011	0.613CysIle: 0.613 ± 0.017	0.491CysLys: 0.491 ± 0.016	0.757CysLeu: 0.757 ± 0.021	0.171CysMet: 0.171 ± 0.011	0.377CysAsn: 0.377 ± 0.012	0.277CysPro: 0.277 ± 0.012	0.203CysGln: 0.203 ± 0.012	0.298CysArg: 0.298 ± 0.012	0.54CysSer: 0.54 ± 0.018	0.418CysThr: 0.418 ± 0.015	0.406CysVal: 0.406 ± 0.016	0.084CysTrp: 0.084 ± 0.007	0.301CysTyr: 0.301 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.77AspAla: 3.77 ± 0.049	0.389AspCys: 0.389 ± 0.015	2.687AspAsp: 2.687 ± 0.048	3.35AspGlu: 3.35 ± 0.053	3.144AspPhe: 3.144 ± 0.046	3.817AspGly: 3.817 ± 0.055	1.041AspHis: 1.041 ± 0.027	4.142AspIle: 4.142 ± 0.049	4.086AspLys: 4.086 ± 0.054	5.449AspLeu: 5.449 ± 0.058	1.258AspMet: 1.258 ± 0.026	2.774AspAsn: 2.774 ± 0.041	2.121AspPro: 2.121 ± 0.035	2.109AspGln: 2.109 ± 0.035	2.195AspArg: 2.195 ± 0.03	3.128AspSer: 3.128 ± 0.042	2.702AspThr: 2.702 ± 0.038	3.478AspVal: 3.478 ± 0.047	0.787AspTrp: 0.787 ± 0.022	2.55AspTyr: 2.55 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	4.116GluAla: 4.116 ± 0.055	0.307GluCys: 0.307 ± 0.014	3.074GluAsp: 3.074 ± 0.039	4.049GluGlu: 4.049 ± 0.062	2.551GluPhe: 2.551 ± 0.04	3.511GluGly: 3.511 ± 0.052	1.116GluHis: 1.116 ± 0.023	4.682GluIle: 4.682 ± 0.067	4.524GluLys: 4.524 ± 0.056	6.084GluLeu: 6.084 ± 0.067	1.499GluMet: 1.499 ± 0.032	3.301GluAsn: 3.301 ± 0.042	1.539GluPro: 1.539 ± 0.032	2.457GluGln: 2.457 ± 0.037	2.571GluArg: 2.571 ± 0.037	3.256GluSer: 3.256 ± 0.04	2.901GluThr: 2.901 ± 0.038	3.917GluVal: 3.917 ± 0.048	0.66GluTrp: 0.66 ± 0.018	2.14GluTyr: 2.14 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.226PheAla: 3.226 ± 0.047	0.43PheCys: 0.43 ± 0.015	2.977PheAsp: 2.977 ± 0.04	2.906PheGlu: 2.906 ± 0.037	2.355PhePhe: 2.355 ± 0.039	3.266PheGly: 3.266 ± 0.051	0.831PheHis: 0.831 ± 0.021	3.671PheIle: 3.671 ± 0.05	3.55PheLys: 3.55 ± 0.044	4.506PheLeu: 4.506 ± 0.052	1.066PheMet: 1.066 ± 0.022	3.025PheAsn: 3.025 ± 0.049	1.738PhePro: 1.738 ± 0.031	1.438PheGln: 1.438 ± 0.026	1.785PheArg: 1.785 ± 0.032	3.936PheSer: 3.936 ± 0.052	2.924PheThr: 2.924 ± 0.043	2.807PheVal: 2.807 ± 0.039	0.564PheTrp: 0.564 ± 0.019	2.027PheTyr: 2.027 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	4.405GlyAla: 4.405 ± 0.055	0.592GlyCys: 0.592 ± 0.018	3.408GlyAsp: 3.408 ± 0.047	3.231GlyGlu: 3.231 ± 0.044	3.607GlyPhe: 3.607 ± 0.046	4.597GlyGly: 4.597 ± 0.072	1.197GlyHis: 1.197 ± 0.026	5.599GlyIle: 5.599 ± 0.065	5.309GlyLys: 5.309 ± 0.065	6.417GlyLeu: 6.417 ± 0.069	1.734GlyMet: 1.734 ± 0.031	3.656GlyAsn: 3.656 ± 0.052	1.538GlyPro: 1.538 ± 0.031	2.045GlyGln: 2.045 ± 0.034	2.481GlyArg: 2.481 ± 0.042	4.496GlySer: 4.496 ± 0.055	4.042GlyThr: 4.042 ± 0.05	4.24GlyVal: 4.24 ± 0.053	0.873GlyTrp: 0.873 ± 0.02	2.984GlyTyr: 2.984 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	1.129HisAla: 1.129 ± 0.027	0.178HisCys: 0.178 ± 0.011	0.923HisAsp: 0.923 ± 0.023	0.992HisGlu: 0.992 ± 0.023	1.078HisPhe: 1.078 ± 0.025	1.099HisGly: 1.099 ± 0.025	0.487HisHis: 0.487 ± 0.021	1.4HisIle: 1.4 ± 0.033	1.049HisLys: 1.049 ± 0.024	1.838HisLeu: 1.838 ± 0.033	0.36HisMet: 0.36 ± 0.015	0.875HisAsn: 0.875 ± 0.023	0.963HisPro: 0.963 ± 0.025	0.804HisGln: 0.804 ± 0.022	0.751HisArg: 0.751 ± 0.021	1.104HisSer: 1.104 ± 0.025	0.962HisThr: 0.962 ± 0.023	0.945HisVal: 0.945 ± 0.026	0.245HisTrp: 0.245 ± 0.012	0.854HisTyr: 0.854 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.184IleAla: 6.184 ± 0.067	0.716IleCys: 0.716 ± 0.02	4.664IleAsp: 4.664 ± 0.05	4.39IleGlu: 4.39 ± 0.048	3.274IlePhe: 3.274 ± 0.052	5.225IleGly: 5.225 ± 0.062	1.342IleHis: 1.342 ± 0.027	5.564IleIle: 5.564 ± 0.08	5.534IleLys: 5.534 ± 0.058	6.811IleLeu: 6.811 ± 0.082	1.465IleMet: 1.465 ± 0.027	4.47IleAsn: 4.47 ± 0.052	3.187IlePro: 3.187 ± 0.041	2.501IleGln: 2.501 ± 0.04	2.894IleArg: 2.894 ± 0.038	5.722IleSer: 5.722 ± 0.062	4.531IleThr: 4.531 ± 0.045	4.624IleVal: 4.624 ± 0.055	0.796IleTrp: 0.796 ± 0.023	2.814IleTyr: 2.814 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	5.21LysAla: 5.21 ± 0.059	0.306LysCys: 0.306 ± 0.013	4.412LysAsp: 4.412 ± 0.061	4.805LysGlu: 4.805 ± 0.061	2.813LysPhe: 2.813 ± 0.042	4.636LysGly: 4.636 ± 0.062	1.384LysHis: 1.384 ± 0.026	5.289LysIle: 5.289 ± 0.064	5.225LysLys: 5.225 ± 0.059	6.59LysLeu: 6.59 ± 0.061	1.851LysMet: 1.851 ± 0.031	4.147LysAsn: 4.147 ± 0.049	2.785LysPro: 2.785 ± 0.045	2.658LysGln: 2.658 ± 0.039	2.834LysArg: 2.834 ± 0.037	4.601LysSer: 4.601 ± 0.048	4.045LysThr: 4.045 ± 0.056	4.705LysVal: 4.705 ± 0.055	0.757LysTrp: 0.757 ± 0.02	2.943LysTyr: 2.943 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	6.777LeuAla: 6.777 ± 0.078	0.779LeuCys: 0.779 ± 0.022	5.022LeuAsp: 5.022 ± 0.062	5.363LeuGlu: 5.363 ± 0.058	4.762LeuPhe: 4.762 ± 0.065	5.871LeuGly: 5.871 ± 0.067	1.647LeuHis: 1.647 ± 0.029	7.322LeuIle: 7.322 ± 0.086	7.705LeuLys: 7.705 ± 0.082	9.667LeuLeu: 9.667 ± 0.109	2.18LeuMet: 2.18 ± 0.033	5.779LeuAsn: 5.779 ± 0.066	3.925LeuPro: 3.925 ± 0.052	3.579LeuGln: 3.579 ± 0.047	3.711LeuArg: 3.711 ± 0.049	7.62LeuSer: 7.62 ± 0.075	5.476LeuThr: 5.476 ± 0.059	5.53LeuVal: 5.53 ± 0.063	0.883LeuTrp: 0.883 ± 0.023	3.192LeuTyr: 3.192 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.714MetAla: 1.714 ± 0.034	0.143MetCys: 0.143 ± 0.008	1.243MetAsp: 1.243 ± 0.022	1.447MetGlu: 1.447 ± 0.03	0.858MetPhe: 0.858 ± 0.025	1.551MetGly: 1.551 ± 0.031	0.419MetHis: 0.419 ± 0.015	1.685MetIle: 1.685 ± 0.03	1.958MetLys: 1.958 ± 0.031	2.262MetLeu: 2.262 ± 0.031	0.668MetMet: 0.668 ± 0.023	1.32MetAsn: 1.32 ± 0.025	1.029MetPro: 1.029 ± 0.026	0.904MetGln: 0.904 ± 0.024	0.995MetArg: 0.995 ± 0.025	1.484MetSer: 1.484 ± 0.028	1.154MetThr: 1.154 ± 0.028	1.491MetVal: 1.491 ± 0.028	0.181MetTrp: 0.181 ± 0.009	0.718MetTyr: 0.718 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.939AsnAla: 3.939 ± 0.051	0.392AsnCys: 0.392 ± 0.016	2.914AsnAsp: 2.914 ± 0.04	3.086AsnGlu: 3.086 ± 0.041	2.697AsnPhe: 2.697 ± 0.037	4.072AsnGly: 4.072 ± 0.058	1.008AsnHis: 1.008 ± 0.024	4.119AsnIle: 4.119 ± 0.055	3.848AsnLys: 3.848 ± 0.055	5.216AsnLeu: 5.216 ± 0.051	1.254AsnMet: 1.254 ± 0.025	3.282AsnAsn: 3.282 ± 0.052	2.634AsnPro: 2.634 ± 0.043	2.016AsnGln: 2.016 ± 0.032	2.272AsnArg: 2.272 ± 0.033	3.574AsnSer: 3.574 ± 0.054	3.343AsnThr: 3.343 ± 0.052	3.326AsnVal: 3.326 ± 0.047	0.729AsnTrp: 0.729 ± 0.02	2.569AsnTyr: 2.569 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.639ProAla: 2.639 ± 0.044	0.222ProCys: 0.222 ± 0.01	2.421ProAsp: 2.421 ± 0.038	2.819ProGlu: 2.819 ± 0.036	1.949ProPhe: 1.949 ± 0.032	2.507ProGly: 2.507 ± 0.036	0.648ProHis: 0.648 ± 0.018	2.765ProIle: 2.765 ± 0.044	2.263ProLys: 2.263 ± 0.041	3.338ProLeu: 3.338 ± 0.044	0.794ProMet: 0.794 ± 0.019	2.006ProAsn: 2.006 ± 0.035	0.923ProPro: 0.923 ± 0.026	1.179ProGln: 1.179 ± 0.026	1.084ProArg: 1.084 ± 0.028	2.461ProSer: 2.461 ± 0.036	1.987ProThr: 1.987 ± 0.038	2.763ProVal: 2.763 ± 0.044	0.394ProTrp: 0.394 ± 0.015	1.493ProTyr: 1.493 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.41GlnAla: 2.41 ± 0.038	0.194GlnCys: 0.194 ± 0.01	1.697GlnAsp: 1.697 ± 0.029	2.083GlnGlu: 2.083 ± 0.034	1.599GlnPhe: 1.599 ± 0.028	1.973GlnGly: 1.973 ± 0.037	0.753GlnHis: 0.753 ± 0.021	2.7GlnIle: 2.7 ± 0.04	2.51GlnLys: 2.51 ± 0.04	3.814GlnLeu: 3.814 ± 0.049	0.896GlnMet: 0.896 ± 0.02	1.987GlnAsn: 1.987 ± 0.031	1.301GlnPro: 1.301 ± 0.027	1.846GlnGln: 1.846 ± 0.038	1.529GlnArg: 1.529 ± 0.031	2.357GlnSer: 2.357 ± 0.035	1.974GlnThr: 1.974 ± 0.036	2.343GlnVal: 2.343 ± 0.034	0.396GlnTrp: 0.396 ± 0.015	1.469GlnTyr: 1.469 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	2.361ArgAla: 2.361 ± 0.036	0.236ArgCys: 0.236 ± 0.012	1.957ArgAsp: 1.957 ± 0.034	2.247ArgGlu: 2.247 ± 0.04	2.119ArgPhe: 2.119 ± 0.035	2.105ArgGly: 2.105 ± 0.038	0.631ArgHis: 0.631 ± 0.018	3.222ArgIle: 3.222 ± 0.046	2.959ArgLys: 2.959 ± 0.045	3.894ArgLeu: 3.894 ± 0.049	1.074ArgMet: 1.074 ± 0.025	2.271ArgAsn: 2.271 ± 0.038	1.305ArgPro: 1.305 ± 0.027	1.359ArgGln: 1.359 ± 0.027	1.521ArgArg: 1.521 ± 0.034	2.451ArgSer: 2.451 ± 0.038	2.011ArgThr: 2.011 ± 0.031	2.377ArgVal: 2.377 ± 0.039	0.453ArgTrp: 0.453 ± 0.018	1.731ArgTyr: 1.731 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.791SerAla: 4.791 ± 0.066	0.586SerCys: 0.586 ± 0.022	3.548SerAsp: 3.548 ± 0.046	3.523SerGlu: 3.523 ± 0.041	3.739SerPhe: 3.739 ± 0.05	4.93SerGly: 4.93 ± 0.056	1.128SerHis: 1.128 ± 0.026	5.393SerIle: 5.393 ± 0.06	4.634SerLys: 4.634 ± 0.058	6.774SerLeu: 6.774 ± 0.07	1.449SerMet: 1.449 ± 0.029	3.641SerAsn: 3.641 ± 0.049	2.412SerPro: 2.412 ± 0.033	2.18SerGln: 2.18 ± 0.035	2.588SerArg: 2.588 ± 0.036	4.953SerSer: 4.953 ± 0.06	3.808SerThr: 3.808 ± 0.048	4.233SerVal: 4.233 ± 0.045	0.83SerTrp: 0.83 ± 0.023	2.997SerTyr: 2.997 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.252ThrAla: 4.252 ± 0.059	0.343ThrCys: 0.343 ± 0.015	3.238ThrAsp: 3.238 ± 0.047	3.092ThrGlu: 3.092 ± 0.048	2.686ThrPhe: 2.686 ± 0.037	4.426ThrGly: 4.426 ± 0.061	0.93ThrHis: 0.93 ± 0.023	4.421ThrIle: 4.421 ± 0.052	3.391ThrLys: 3.391 ± 0.046	5.41ThrLeu: 5.41 ± 0.06	1.097ThrMet: 1.097 ± 0.025	2.971ThrAsn: 2.971 ± 0.047	2.493ThrPro: 2.493 ± 0.046	1.77ThrGln: 1.77 ± 0.03	1.831ThrArg: 1.831 ± 0.029	3.772ThrSer: 3.772 ± 0.047	3.335ThrThr: 3.335 ± 0.057	3.68ThrVal: 3.68 ± 0.049	0.596ThrTrp: 0.596 ± 0.018	2.365ThrTyr: 2.365 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	4.441ValAla: 4.441 ± 0.053	0.525ValCys: 0.525 ± 0.018	3.452ValAsp: 3.452 ± 0.048	3.576ValGlu: 3.576 ± 0.044	3.06ValPhe: 3.06 ± 0.046	3.799ValGly: 3.799 ± 0.048	1.037ValHis: 1.037 ± 0.022	4.938ValIle: 4.938 ± 0.058	4.521ValLys: 4.521 ± 0.054	6.067ValLeu: 6.067 ± 0.068	1.504ValMet: 1.504 ± 0.027	3.682ValAsn: 3.682 ± 0.046	2.321ValPro: 2.321 ± 0.035	1.957ValGln: 1.957 ± 0.032	2.314ValArg: 2.314 ± 0.039	4.569ValSer: 4.569 ± 0.055	3.576ValThr: 3.576 ± 0.055	4.25ValVal: 4.25 ± 0.051	0.65ValTrp: 0.65 ± 0.019	2.448ValTyr: 2.448 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.746TrpAla: 0.746 ± 0.022	0.106TrpCys: 0.106 ± 0.008	0.623TrpAsp: 0.623 ± 0.019	0.607TrpGlu: 0.607 ± 0.019	0.586TrpPhe: 0.586 ± 0.018	0.776TrpGly: 0.776 ± 0.021	0.238TrpHis: 0.238 ± 0.013	0.798TrpIle: 0.798 ± 0.022	0.863TrpLys: 0.863 ± 0.023	1.109TrpLeu: 1.109 ± 0.025	0.327TrpMet: 0.327 ± 0.014	0.648TrpAsn: 0.648 ± 0.021	0.327TrpPro: 0.327 ± 0.014	0.469TrpGln: 0.469 ± 0.015	0.457TrpArg: 0.457 ± 0.015	0.708TrpSer: 0.708 ± 0.021	0.581TrpThr: 0.581 ± 0.021	0.661TrpVal: 0.661 ± 0.02	0.169TrpTrp: 0.169 ± 0.01	0.453TrpTyr: 0.453 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.74TyrAla: 2.74 ± 0.039	0.359TyrCys: 0.359 ± 0.013	2.254TyrAsp: 2.254 ± 0.037	2.063TyrGlu: 2.063 ± 0.036	2.306TyrPhe: 2.306 ± 0.04	2.643TyrGly: 2.643 ± 0.037	0.865TyrHis: 0.865 ± 0.023	2.644TyrIle: 2.644 ± 0.037	2.735TyrLys: 2.735 ± 0.038	3.926TyrLeu: 3.926 ± 0.048	0.799TyrMet: 0.799 ± 0.019	2.379TyrAsn: 2.379 ± 0.039	1.666TyrPro: 1.666 ± 0.033	1.681TyrGln: 1.681 ± 0.029	1.863TyrArg: 1.863 ± 0.033	2.807TyrSer: 2.807 ± 0.044	2.393TyrThr: 2.393 ± 0.041	2.275TyrVal: 2.275 ± 0.04	0.504TyrTrp: 0.504 ± 0.017	1.766TyrTyr: 1.766 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 6611 proteins (1828152 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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