Gordonia phage Syleon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WGL6|A0A5Q2WGL6_9CAUD Uncharacterized protein OS=Gordonia phage Syleon OX=2653718 GN=70 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.56LALSLLSEE11 pKa = 4.21SLIGKK16 pKa = 8.39RR17 pKa = 11.84VKK19 pKa = 10.5SATKK23 pKa = 10.71SNLTLDD29 pKa = 4.24DD30 pKa = 3.78GTVIEE35 pKa = 4.97LFEE38 pKa = 4.45SAQDD42 pKa = 3.72CCAAAYY48 pKa = 10.1GDD50 pKa = 3.85WALADD55 pKa = 4.52AVDD58 pKa = 4.7LDD60 pKa = 4.03AAITAVEE67 pKa = 4.24LTSEE71 pKa = 4.09EE72 pKa = 4.38HH73 pKa = 6.58EE74 pKa = 5.58DD75 pKa = 3.74YY76 pKa = 10.95DD77 pKa = 3.62SHH79 pKa = 5.58YY80 pKa = 9.97TSGEE84 pKa = 3.49LRR86 pKa = 11.84IMHH89 pKa = 6.11NQNPIALGDD98 pKa = 3.58LHH100 pKa = 8.14AGAGNGGYY108 pKa = 9.63YY109 pKa = 10.51YY110 pKa = 10.82SVLSVKK116 pKa = 9.89VHH118 pKa = 6.51LPTGGSVEE126 pKa = 4.29TEE128 pKa = 4.58LISSS132 pKa = 4.26

Molecular weight: 13.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WBL2|A0A5Q2WBL2_9CAUD AAA-ATPase OS=Gordonia phage Syleon OX=2653718 GN=123 PE=4 SV=1
MM1 pKa = 7.85GIHH4 pKa = 7.12KK5 pKa = 10.05GRR7 pKa = 11.84SGRR10 pKa = 11.84PYY12 pKa = 7.39RR13 pKa = 11.84TKK15 pKa = 9.66RR16 pKa = 11.84ARR18 pKa = 11.84YY19 pKa = 8.65RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.52CAAEE26 pKa = 3.89KK27 pKa = 10.47RR28 pKa = 11.84PCSLCGKK35 pKa = 8.97PIDD38 pKa = 3.75YY39 pKa = 9.04TLTGKK44 pKa = 9.83HH45 pKa = 5.19PMAWTLEE52 pKa = 4.16HH53 pKa = 7.15IIPVSEE59 pKa = 5.22LKK61 pKa = 10.65RR62 pKa = 11.84IDD64 pKa = 3.57PNHH67 pKa = 6.88RR68 pKa = 11.84LLEE71 pKa = 4.36AEE73 pKa = 4.67SNFEE77 pKa = 4.01AAHH80 pKa = 5.31MLCNQRR86 pKa = 11.84KK87 pKa = 8.4QDD89 pKa = 2.88GGYY92 pKa = 9.1RR93 pKa = 11.84AKK95 pKa = 10.76GGAGPAPAASSRR107 pKa = 11.84RR108 pKa = 11.84WTT110 pKa = 3.42

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

138

0

138

23305

24

2165

168.9

18.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.534 ± 0.352

0.944 ± 0.119

6.599 ± 0.189

6.449 ± 0.251

3.308 ± 0.143

8.26 ± 0.323

2.012 ± 0.146

4.36 ± 0.142

4.497 ± 0.197

8.38 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.1

3.308 ± 0.157

5.033 ± 0.249

3.368 ± 0.164

6.312 ± 0.19

6.02 ± 0.234

5.81 ± 0.203

7.432 ± 0.216

2.047 ± 0.103

2.965 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski