Thiosulfativibrio zosterae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Thiosulfativibrio

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F8PKM1|A0A6F8PKM1_9GAMM tRNA pseudouridine synthase D OS=Thiosulfativibrio zosterae OX=2675053 GN=truD PE=3 SV=1
MM1 pKa = 7.24NCKK4 pKa = 9.77LHH6 pKa = 5.6YY7 pKa = 9.84PKK9 pKa = 10.77VFLISLFIGGLSGCDD24 pKa = 3.19SPSKK28 pKa = 10.71LDD30 pKa = 3.43AEE32 pKa = 5.14GIGKK36 pKa = 9.82YY37 pKa = 10.02ISGVGNIVMSGLVVDD52 pKa = 4.58PAIGGATVYY61 pKa = 10.44IDD63 pKa = 3.7ANGDD67 pKa = 3.67GVIDD71 pKa = 5.14DD72 pKa = 5.51NDD74 pKa = 3.81PQAQTDD80 pKa = 3.6SLGYY84 pKa = 10.2FNYY87 pKa = 10.65NPVTNVDD94 pKa = 3.6YY95 pKa = 11.17CAVGPSAYY103 pKa = 9.49CFPMKK108 pKa = 10.3EE109 pKa = 4.02EE110 pKa = 3.96YY111 pKa = 10.39AGQVLRR117 pKa = 11.84SSGGTDD123 pKa = 3.15LVTNKK128 pKa = 9.65PFEE131 pKa = 4.52GEE133 pKa = 4.08MQLKK137 pKa = 10.35LSSEE141 pKa = 4.16VNEE144 pKa = 4.32NQTEE148 pKa = 4.67GIVITPLSSIVAFMTDD164 pKa = 3.15EE165 pKa = 4.14QKK167 pKa = 11.44ASLKK171 pKa = 10.85SLLNLNEE178 pKa = 4.15SDD180 pKa = 5.43DD181 pKa = 4.25LNKK184 pKa = 10.26LDD186 pKa = 5.28PLDD189 pKa = 4.63FSDD192 pKa = 4.84AKK194 pKa = 11.19YY195 pKa = 8.05PTAEE199 pKa = 4.69DD200 pKa = 3.73KK201 pKa = 11.17AKK203 pKa = 9.92QQQRR207 pKa = 11.84LKK209 pKa = 10.67IIVNIQNTVQALTSALATSDD229 pKa = 3.14KK230 pKa = 9.76TQNNALNEE238 pKa = 3.83AVYY241 pKa = 10.54AVVAQNVTSFTTDD254 pKa = 2.89LQRR257 pKa = 11.84ADD259 pKa = 3.83NQPTNTDD266 pKa = 3.02NSVDD270 pKa = 3.7SLLDD274 pKa = 3.3KK275 pKa = 11.16VIIQVSQSTKK285 pKa = 9.66VSNAVKK291 pKa = 8.79TQAQSLSTTSPNANPTTRR309 pKa = 11.84NALLNNVSALSAASNNVFNTTTITTNANASKK340 pKa = 10.81VLFKK344 pKa = 10.9TEE346 pKa = 4.2VVGEE350 pKa = 4.38TVNATPFDD358 pKa = 3.56ITNTQTAITIINSTVVNNLIDD379 pKa = 4.09GSTADD384 pKa = 4.05FTQLTNIAITQNITSEE400 pKa = 4.29EE401 pKa = 4.07EE402 pKa = 3.6LGGIIDD408 pKa = 4.13SGVGTGDD415 pKa = 3.62PVPGNTDD422 pKa = 2.47IGTTTGDD429 pKa = 3.14GTTTGDD435 pKa = 3.19GTTTGDD441 pKa = 3.19GTTTGDD447 pKa = 3.19GTTTGDD453 pKa = 3.17GTTTGGGGTTIPPITNTAPVAVDD476 pKa = 3.59DD477 pKa = 5.21SITAIEE483 pKa = 4.28DD484 pKa = 3.81TVFTSTIDD492 pKa = 3.7LDD494 pKa = 5.31ANDD497 pKa = 4.75TDD499 pKa = 5.09LDD501 pKa = 4.56SNTLSVVAGTFSTAQGGTLILASNGSYY528 pKa = 10.11TYY530 pKa = 11.14LSATNFNGTDD540 pKa = 3.28TVDD543 pKa = 3.34YY544 pKa = 9.75TVTDD548 pKa = 3.86GALTDD553 pKa = 3.89IGTLTITVEE562 pKa = 4.23PVNDD566 pKa = 3.52LPTANGEE573 pKa = 4.52SITVSKK579 pKa = 10.62DD580 pKa = 3.03VEE582 pKa = 4.69YY583 pKa = 10.62ISSTVNLLANDD594 pKa = 3.94TDD596 pKa = 3.63VDD598 pKa = 4.11GDD600 pKa = 4.0DD601 pKa = 4.12LSIVSGTYY609 pKa = 6.36TTQAGGSITLNANGSFSYY627 pKa = 10.55TPAANFNGTDD637 pKa = 3.13AYY639 pKa = 10.95VYY641 pKa = 10.0EE642 pKa = 4.31VSDD645 pKa = 4.14DD646 pKa = 3.93GGVNASATLTIIVTDD661 pKa = 3.85AMSDD665 pKa = 3.17ADD667 pKa = 4.01EE668 pKa = 4.51TVTTAEE674 pKa = 4.19DD675 pKa = 3.34TALTGNVMSNLTDD688 pKa = 3.97ADD690 pKa = 4.06SSSHH694 pKa = 5.81SVTSFTVEE702 pKa = 3.77GQTYY706 pKa = 9.62QPGSTATITGGTITIAATGAYY727 pKa = 7.86TFTSSEE733 pKa = 4.08NFNGSVPAISYY744 pKa = 11.46AMVDD748 pKa = 3.27NTDD751 pKa = 4.11ANDD754 pKa = 3.81TDD756 pKa = 3.8TSTLNISVTAVTDD769 pKa = 3.58AMSDD773 pKa = 3.22ADD775 pKa = 4.02EE776 pKa = 4.5TLTTAEE782 pKa = 4.27DD783 pKa = 3.5TALTGNVMSNLTDD796 pKa = 3.97ADD798 pKa = 4.06SSSHH802 pKa = 5.81SVTSFTVEE810 pKa = 3.77GQTYY814 pKa = 9.62QPGSTATITGGTITIAATGAYY835 pKa = 7.9TFTPSEE841 pKa = 4.27NFNGTVPAISYY852 pKa = 11.5AMVDD856 pKa = 3.68NNDD859 pKa = 3.32ATDD862 pKa = 3.67TDD864 pKa = 4.0TSTLNISVNSVDD876 pKa = 4.1DD877 pKa = 3.87VPVAVEE883 pKa = 5.59DD884 pKa = 3.87NFTLNEE890 pKa = 4.65DD891 pKa = 3.76GAITDD896 pKa = 3.92TLATNDD902 pKa = 4.06TLSGDD907 pKa = 3.63GGNVFTKK914 pKa = 10.1ATDD917 pKa = 3.81PAHH920 pKa = 5.71GTVTVNADD928 pKa = 3.18GTFTYY933 pKa = 10.34TPAANYY939 pKa = 10.16NGADD943 pKa = 3.52SFSYY947 pKa = 10.46TITDD951 pKa = 3.41ADD953 pKa = 4.36GDD955 pKa = 3.92TSTAVVNLTVNSVDD969 pKa = 3.8DD970 pKa = 3.92VPVAVEE976 pKa = 5.59DD977 pKa = 3.87NFTLNEE983 pKa = 4.65DD984 pKa = 3.76GAITDD989 pKa = 3.92TLATNDD995 pKa = 4.06TLSGDD1000 pKa = 3.63GGNVFTKK1007 pKa = 9.63EE1008 pKa = 3.71TDD1010 pKa = 3.62PAHH1013 pKa = 5.72GTVTVNADD1021 pKa = 3.18GTFTYY1026 pKa = 10.34TPAANYY1032 pKa = 10.16NGADD1036 pKa = 3.52SFSYY1040 pKa = 10.46TITDD1044 pKa = 3.41ADD1046 pKa = 4.36GDD1048 pKa = 3.92TSTAVVNITVVAVTNAMSDD1067 pKa = 3.24ADD1069 pKa = 3.96EE1070 pKa = 4.32TLSTNEE1076 pKa = 3.81DD1077 pKa = 3.38TALSGNVMSNLTDD1090 pKa = 3.97ADD1092 pKa = 4.06SSSHH1096 pKa = 5.78SVTSFSVAGVATSYY1110 pKa = 11.0TPGQTATIVNLGTITIATNGDD1131 pKa = 3.72YY1132 pKa = 10.95TFTPAANFNGSVPAISYY1149 pKa = 11.56AMVDD1153 pKa = 3.47NNDD1156 pKa = 3.84ANDD1159 pKa = 3.91TDD1161 pKa = 3.92TSTLNITVDD1170 pKa = 3.65AVNDD1174 pKa = 3.89SPVALLASTSSPIIGNPFDD1193 pKa = 4.74LEE1195 pKa = 4.33SGLTAEE1201 pKa = 5.72GLIEE1205 pKa = 4.66LDD1207 pKa = 3.04NHH1209 pKa = 6.85KK1210 pKa = 10.49ILIYY1214 pKa = 10.84GLDD1217 pKa = 3.73KK1218 pKa = 11.05INNKK1222 pKa = 10.2LYY1224 pKa = 9.55ISRR1227 pKa = 11.84WFANGRR1233 pKa = 11.84VDD1235 pKa = 3.39TTYY1238 pKa = 9.58GTDD1241 pKa = 4.25GILTVNVDD1249 pKa = 3.55ANAEE1253 pKa = 3.74IDD1255 pKa = 3.85AVKK1258 pKa = 10.73QSDD1261 pKa = 3.7GKK1263 pKa = 11.25VNFLIAYY1270 pKa = 5.91PTAGAAQIKK1279 pKa = 9.9IMQLDD1284 pKa = 3.63SEE1286 pKa = 4.9GVVNITFGTSGVKK1299 pKa = 9.19QLSIINSPSTFTQVRR1314 pKa = 11.84LLMQGTKK1321 pKa = 9.69MIVLGLDD1328 pKa = 3.42DD1329 pKa = 4.3SSNTLVMRR1337 pKa = 11.84LLSTGVYY1344 pKa = 7.99DD1345 pKa = 3.69TTFGDD1350 pKa = 3.83SSNGLAYY1357 pKa = 10.4GEE1359 pKa = 4.62GFLDD1363 pKa = 3.68NLSDD1367 pKa = 3.94VVIGSDD1373 pKa = 3.22DD1374 pKa = 3.61TIYY1377 pKa = 11.63YY1378 pKa = 8.6MGAYY1382 pKa = 9.98NSDD1385 pKa = 2.88IFISKK1390 pKa = 10.29LNADD1394 pKa = 3.8GTINAAFNSNARR1406 pKa = 11.84LEE1408 pKa = 4.62LIIDD1412 pKa = 3.65ALGVFTASNLVLDD1425 pKa = 5.5GSALMYY1431 pKa = 10.88LLAYY1435 pKa = 9.58KK1436 pKa = 10.78DD1437 pKa = 3.55EE1438 pKa = 4.84DD1439 pKa = 4.37SNNKK1443 pKa = 9.88LILGKK1448 pKa = 10.56VSAADD1453 pKa = 3.89GSAVIDD1459 pKa = 3.82SYY1461 pKa = 11.84FSLGYY1466 pKa = 10.3KK1467 pKa = 9.89ILDD1470 pKa = 3.97LSDD1473 pKa = 3.86APQMFPTSVEE1483 pKa = 3.88MLPNNGLLIGGYY1495 pKa = 9.67NPNTVNSISNTLKK1508 pKa = 10.44LAKK1511 pKa = 9.96FNYY1514 pKa = 8.56STGDD1518 pKa = 3.4LEE1520 pKa = 4.89YY1521 pKa = 10.85SFSGNAGVYY1530 pKa = 10.13ISQSVGKK1537 pKa = 10.31VSDD1540 pKa = 3.22IALIGTNNDD1549 pKa = 4.16EE1550 pKa = 3.62IWMLGDD1556 pKa = 3.66RR1557 pKa = 11.84LSEE1560 pKa = 4.21SVGNNGTFNITRR1572 pKa = 11.84LDD1574 pKa = 3.55LGGVVVNYY1582 pKa = 10.74DD1583 pKa = 3.33LAYY1586 pKa = 10.57DD1587 pKa = 3.86VNNGVLPTAVSVSIPAFDD1605 pKa = 3.97VEE1607 pKa = 4.81GDD1609 pKa = 3.33NFTYY1613 pKa = 10.87SLLGTASNAASVTLDD1628 pKa = 3.21SATGEE1633 pKa = 3.93LSYY1636 pKa = 11.84SNDD1639 pKa = 2.72NCYY1642 pKa = 10.56AAGTQVDD1649 pKa = 4.39GLGHH1653 pKa = 6.42YY1654 pKa = 10.18YY1655 pKa = 11.0DD1656 pKa = 5.38LVDD1659 pKa = 5.58FNMTDD1664 pKa = 3.16SQGATSSTQTFTVRR1678 pKa = 11.84LFNCGAA1684 pKa = 3.72

Molecular weight: 174.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F8PRG8|A0A6F8PRG8_9GAMM Probable membrane transporter protein OS=Thiosulfativibrio zosterae OX=2675053 GN=THMIRHAT_23730 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.93RR41 pKa = 11.84LTVSDD46 pKa = 3.89KK47 pKa = 11.45

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2362

0

2362

802121

45

9540

339.6

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.946 ± 0.053

0.786 ± 0.018

5.333 ± 0.092

6.214 ± 0.05

4.195 ± 0.037

6.475 ± 0.051

2.248 ± 0.035

6.305 ± 0.043

5.724 ± 0.047

10.844 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.037

4.35 ± 0.044

4.044 ± 0.039

5.371 ± 0.058

3.953 ± 0.055

6.132 ± 0.047

5.47 ± 0.144

6.729 ± 0.045

1.23 ± 0.023

2.879 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski