Deinococcus peraridilitoris (strain DSM 19664 / LMG 22246 / CIP 109416 / KR-200)
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4164 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0A8N8|L0A8N8_DEIPD ABC-type dipeptide/oligopeptide/nickel transport system permease component OS=Deinococcus peraridilitoris (strain DSM 19664 / LMG 22246 / CIP 109416 / KR-200) OX=937777 GN=Deipe_4053 PE=3 SV=1
MM1 pKa = 7.12 PQYY4 pKa = 10.91 LIVLEE9 pKa = 4.58 PGANNWSAYY18 pKa = 10.46 APDD21 pKa = 3.9 LPGVVTTGKK30 pKa = 10.35 DD31 pKa = 3.25 ADD33 pKa = 4.0 DD34 pKa = 3.44 TTEE37 pKa = 3.89 RR38 pKa = 11.84 MRR40 pKa = 11.84 EE41 pKa = 3.85 AIAAHH46 pKa = 7.05 LYY48 pKa = 9.77 EE49 pKa = 4.53 LQQDD53 pKa = 3.53 GDD55 pKa = 4.59 EE56 pKa = 4.61 IPVPTSTPEE65 pKa = 3.86 THH67 pKa = 6.87 VSADD71 pKa = 3.78 LGNVLCMIEE80 pKa = 3.98 PAPP83 pKa = 4.15
Molecular weight: 8.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.973
IPC_protein 3.872
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.605
Solomon 3.834
Lehninger 3.795
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.745
EMBOSS 3.795
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|L0A9H2|L0A9H2_DEIPD Uncharacterized protein OS=Deinococcus peraridilitoris (strain DSM 19664 / LMG 22246 / CIP 109416 / KR-200) OX=937777 GN=Deipe_4355 PE=4 SV=1
MM1 pKa = 7.64 ILPAEE6 pKa = 4.14 KK7 pKa = 10.44 AGISVRR13 pKa = 11.84 RR14 pKa = 11.84 QCQLLKK20 pKa = 10.44 VNRR23 pKa = 11.84 ASVHH27 pKa = 4.25 YY28 pKa = 10.29 QKK30 pKa = 9.6 TLKK33 pKa = 10.39 RR34 pKa = 11.84 EE35 pKa = 3.91 RR36 pKa = 11.84 TQGINDD42 pKa = 3.49 DD43 pKa = 3.75 QLRR46 pKa = 11.84 QEE48 pKa = 4.26 IEE50 pKa = 4.17 QVILDD55 pKa = 3.89 CSGYY59 pKa = 8.79 GYY61 pKa = 10.71 RR62 pKa = 11.84 RR63 pKa = 11.84 VTHH66 pKa = 5.57 EE67 pKa = 3.55 LGRR70 pKa = 11.84 RR71 pKa = 11.84 HH72 pKa = 5.75 RR73 pKa = 11.84 QVNHH77 pKa = 3.87 MRR79 pKa = 11.84 VRR81 pKa = 11.84 RR82 pKa = 11.84 VMKK85 pKa = 9.57 QHH87 pKa = 5.88 QLQCRR92 pKa = 11.84 VRR94 pKa = 11.84 PRR96 pKa = 11.84 KK97 pKa = 10.01 KK98 pKa = 9.29 NTPGLPAAGIPNLLRR113 pKa = 11.84 DD114 pKa = 4.01 EE115 pKa = 5.18 KK116 pKa = 10.38 IVPSGPDD123 pKa = 3.13 QVWIEE128 pKa = 4.01 ARR130 pKa = 11.84 PGRR133 pKa = 11.84 PCPGEE138 pKa = 3.74 PLACGLHH145 pKa = 6.05 LRR147 pKa = 11.84 ARR149 pKa = 11.84 PDD151 pKa = 3.22 GFRR154 pKa = 11.84 VPGLRR159 pKa = 11.84 TRR161 pKa = 11.84 RR162 pKa = 11.84 VFKK165 pKa = 10.27 EE166 pKa = 3.39 DD167 pKa = 3.02 RR168 pKa = 11.84 GLGFVVTSGRR178 pKa = 11.84 AAGVCCIEE186 pKa = 5.69 DD187 pKa = 3.65 GNQRR191 pKa = 11.84 AGSVAGLIHH200 pKa = 6.98 HH201 pKa = 6.98 SDD203 pKa = 3.16 QGSVYY208 pKa = 10.47 QSGAYY213 pKa = 9.43 LALLDD218 pKa = 4.02
Molecular weight: 24.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.487
IPC_protein 10.233
Toseland 10.496
ProMoST 10.204
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.76
Grimsley 10.657
Solomon 10.716
Lehninger 10.687
Nozaki 10.526
DTASelect 10.321
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.452
IPC_peptide 10.716
IPC2_peptide 9.633
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4164
0
4164
1261125
29
3232
302.9
33.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.4 ± 0.048
0.652 ± 0.011
4.941 ± 0.028
6.021 ± 0.039
3.433 ± 0.022
8.547 ± 0.038
2.339 ± 0.024
3.739 ± 0.029
2.637 ± 0.031
11.622 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.892 ± 0.016
2.669 ± 0.026
5.302 ± 0.034
4.002 ± 0.028
7.635 ± 0.038
5.623 ± 0.033
5.739 ± 0.038
7.949 ± 0.031
1.428 ± 0.016
2.429 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here