Colletotrichum fioriniae PJ7
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13759 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A010RL95|A0A010RL95_9PEZI U6 snRNA-associated Sm-like protein LSm3 OS=Colletotrichum fioriniae PJ7 OX=1445577 GN=LSM3 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.3 LDD4 pKa = 4.17 AEE6 pKa = 4.83 LFYY9 pKa = 11.16 QEE11 pKa = 4.04 NWADD15 pKa = 3.49 STYY18 pKa = 11.01 TLSNWGDD25 pKa = 3.38 STIWEE30 pKa = 4.4 WGTKK34 pKa = 9.38 AVANVANLSTDD45 pKa = 2.67 WSNLTDD51 pKa = 4.55 GLAEE55 pKa = 4.35 DD56 pKa = 4.89 TPHH59 pKa = 7.58 GDD61 pKa = 3.05 ACADD65 pKa = 3.67 NIARR69 pKa = 11.84 DD70 pKa = 4.01 DD71 pKa = 3.9 NGVCVTGDD79 pKa = 3.37 ASTDD83 pKa = 2.72 ICAGFDD89 pKa = 3.48 TFSVHH94 pKa = 6.97 SLDD97 pKa = 3.97 RR98 pKa = 11.84 VSTEE102 pKa = 4.83 DD103 pKa = 3.27 IADD106 pKa = 3.41 VGNFILVNSSTEE118 pKa = 3.94 DD119 pKa = 3.26 LSAEE123 pKa = 4.17 NGGSVTPCHH132 pKa = 6.37
Molecular weight: 14.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.401
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.312
Solomon 3.617
Lehninger 3.579
Nozaki 3.757
DTASelect 4.024
Thurlkill 3.478
EMBOSS 3.617
Sillero 3.757
Patrickios 0.896
IPC_peptide 3.617
IPC2_peptide 3.719
IPC2.peptide.svr19 3.686
Protein with the highest isoelectric point:
>tr|A0A010RBN8|A0A010RBN8_9PEZI Protein kinase OS=Colletotrichum fioriniae PJ7 OX=1445577 GN=CFIO01_10155 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.82 PLRR5 pKa = 11.84 LTTSRR10 pKa = 11.84 PVSPTTVVRR19 pKa = 11.84 RR20 pKa = 11.84 PRR22 pKa = 11.84 RR23 pKa = 11.84 LRR25 pKa = 11.84 PPSVRR30 pKa = 11.84 SLPSTRR36 pKa = 11.84 LSRR39 pKa = 11.84 LVTLRR44 pKa = 11.84 LVSPTTAARR53 pKa = 11.84 RR54 pKa = 11.84 PRR56 pKa = 11.84 RR57 pKa = 11.84 PRR59 pKa = 11.84 PPSVRR64 pKa = 11.84 SLLSMRR70 pKa = 11.84 PSRR73 pKa = 11.84 PATLSLASLTTAARR87 pKa = 11.84 RR88 pKa = 11.84 PRR90 pKa = 11.84 RR91 pKa = 11.84 PRR93 pKa = 11.84 PPSATNPPSTRR104 pKa = 11.84 PSRR107 pKa = 11.84 LVTSKK112 pKa = 10.16 PASPTTAARR121 pKa = 11.84 RR122 pKa = 11.84 RR123 pKa = 11.84 RR124 pKa = 11.84 RR125 pKa = 11.84 PRR127 pKa = 11.84 LPSVTNSSPRR137 pKa = 11.84 RR138 pKa = 11.84 PSSLLSTRR146 pKa = 11.84 SRR148 pKa = 11.84 PVSPTTAARR157 pKa = 11.84 RR158 pKa = 11.84 PRR160 pKa = 11.84 RR161 pKa = 11.84 RR162 pKa = 11.84 RR163 pKa = 11.84 PPSAMSPPPTTPSSPPSTRR182 pKa = 11.84 SRR184 pKa = 11.84 PASLTTVARR193 pKa = 11.84 RR194 pKa = 11.84 PRR196 pKa = 11.84 RR197 pKa = 11.84 QRR199 pKa = 11.84 LLSARR204 pKa = 11.84 SPPPTTPLSTPSRR217 pKa = 11.84 PASPTTVXXXRR228 pKa = 11.84 RR229 pKa = 11.84 PRR231 pKa = 11.84 PRR233 pKa = 11.84 SATSSLPRR241 pKa = 11.84 TLPSTPLRR249 pKa = 11.84 LVSPTTAARR258 pKa = 11.84 RR259 pKa = 11.84 PRR261 pKa = 11.84 RR262 pKa = 11.84 PRR264 pKa = 11.84 PRR266 pKa = 11.84 SATSTPPLRR275 pKa = 11.84 LALSPLKK282 pKa = 10.79 GVVQLCGRR290 pKa = 11.84 HH291 pKa = 6.26 GGQQLNALTRR301 pKa = 11.84 ATLEE305 pKa = 3.79 LRR307 pKa = 11.84 RR308 pKa = 11.84 PPVLPCII315 pKa = 4.39
Molecular weight: 34.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.72
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.337
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13759
0
13759
6816730
24
9111
495.4
54.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.073 ± 0.019
1.217 ± 0.007
5.714 ± 0.014
6.03 ± 0.023
3.79 ± 0.012
7.152 ± 0.02
2.312 ± 0.011
4.795 ± 0.014
4.804 ± 0.017
8.678 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.21 ± 0.008
3.672 ± 0.01
6.102 ± 0.026
3.889 ± 0.014
5.864 ± 0.02
8.067 ± 0.025
6.11 ± 0.025
6.255 ± 0.018
1.538 ± 0.008
2.725 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here