Rhesus monkey rhadinovirus H26-95
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q77NI9|Q77NI9_9GAMA ORF52 OS=Rhesus monkey rhadinovirus H26-95 OX=69256 GN=ORF52 PE=3 SV=1
MM1 pKa = 7.27 TGSIVLALALLACLYY16 pKa = 10.8 LCLPVCATVTTSSTTGTGTPPVTTNPSAAPSVTPSFYY53 pKa = 10.66 DD54 pKa = 3.56 YY55 pKa = 11.29 DD56 pKa = 4.35 CSADD60 pKa = 3.26 TYY62 pKa = 11.31 QPVLSSFSSIWAVINSVLVAVATFLYY88 pKa = 8.44 LTYY91 pKa = 9.62 MCFFKK96 pKa = 10.81 FVEE99 pKa = 5.05 TVAHH103 pKa = 5.99 EE104 pKa = 4.0
Molecular weight: 11.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.054
IPC2_protein 4.215
IPC_protein 3.923
Toseland 3.757
ProMoST 3.999
Dawson 3.935
Bjellqvist 4.291
Wikipedia 3.91
Rodwell 3.77
Grimsley 3.681
Solomon 3.884
Lehninger 3.834
Nozaki 4.101
DTASelect 4.24
Thurlkill 3.859
EMBOSS 3.923
Sillero 4.05
Patrickios 0.006
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 4.171
Protein with the highest isoelectric point:
>tr|Q9J2H1|Q9J2H1_9GAMA Cyclin D homolog OS=Rhesus monkey rhadinovirus H26-95 OX=69256 GN=ORF72 PE=3 SV=1
MM1 pKa = 7.15 WGSRR5 pKa = 11.84 QHH7 pKa = 6.75 RR8 pKa = 11.84 SGIVSGHH15 pKa = 5.78 GLRR18 pKa = 11.84 SSCRR22 pKa = 11.84 GHH24 pKa = 6.48 CGRR27 pKa = 11.84 RR28 pKa = 11.84 GGTRR32 pKa = 11.84 EE33 pKa = 3.5 QAGRR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 GRR42 pKa = 11.84 LGRR45 pKa = 11.84 ASLPAPTPPAPTTSGPQVRR64 pKa = 11.84 AVAEE68 pKa = 4.08 QGHH71 pKa = 6.07 GSDD74 pKa = 4.09 TEE76 pKa = 4.21 TATEE80 pKa = 4.15 SRR82 pKa = 11.84 HH83 pKa = 5.67 GSSQGSPSGSGSEE96 pKa = 4.18 SVIVLGSPTPSPSGSAPVLASGLSPRR122 pKa = 11.84 NTSGSSPASPASHH135 pKa = 6.74 SPPPSPPSHH144 pKa = 7.34 PGPHH148 pKa = 6.08 SPAPPSSHH156 pKa = 6.3 NPSPNQQPSSFLQPSPHH173 pKa = 7.33 DD174 pKa = 3.77 SPEE177 pKa = 4.13 PPEE180 pKa = 4.97 PPTSLPPPDD189 pKa = 4.45 SPGPPQSPTPTSSPPPQSPPDD210 pKa = 3.79 SPEE213 pKa = 4.23 PPQSPTPQQAPSPNTQQAVSHH234 pKa = 6.71 TDD236 pKa = 2.93 HH237 pKa = 7.02 PTGPSRR243 pKa = 11.84 PGPPFPGHH251 pKa = 6.04 TSHH254 pKa = 7.1 SYY256 pKa = 9.39 TVGGWGPPTRR266 pKa = 11.84 AGGVPCLRR274 pKa = 11.84 LRR276 pKa = 11.84 CTSHH280 pKa = 6.87 NSHH283 pKa = 6.7 EE284 pKa = 4.5 DD285 pKa = 3.42 EE286 pKa = 4.63 APEE289 pKa = 3.91 RR290 pKa = 11.84 QQEE293 pKa = 4.17 QEE295 pKa = 3.97 GEE297 pKa = 4.1 EE298 pKa = 4.25 RR299 pKa = 11.84 QQQPARR305 pKa = 11.84 PPRR308 pKa = 11.84 PPRR311 pKa = 11.84 PPRR314 pKa = 11.84 YY315 pKa = 8.48 PIPIPYY321 pKa = 9.16 PSSEE325 pKa = 4.01 EE326 pKa = 3.88 EE327 pKa = 4.06 VPRR330 pKa = 11.84 KK331 pKa = 9.69 YY332 pKa = 10.3 RR333 pKa = 11.84 PQRR336 pKa = 11.84 RR337 pKa = 11.84 FYY339 pKa = 10.68 RR340 pKa = 11.84 QVLGPRR346 pKa = 11.84 IDD348 pKa = 3.73 PPRR351 pKa = 11.84 PGPWCHH357 pKa = 6.42 GVIFCNSDD365 pKa = 3.2 PYY367 pKa = 11.37 SLYY370 pKa = 10.78 RR371 pKa = 11.84 LARR374 pKa = 11.84 CLQFPGIRR382 pKa = 11.84 ASSVRR387 pKa = 11.84 VLPDD391 pKa = 3.26 APGSPVIPAFCITVFCQSRR410 pKa = 11.84 GTAKK414 pKa = 10.28 AVKK417 pKa = 9.25 KK418 pKa = 10.48 ARR420 pKa = 11.84 RR421 pKa = 11.84 RR422 pKa = 11.84 WEE424 pKa = 3.82 RR425 pKa = 11.84 HH426 pKa = 5.73 HH427 pKa = 7.05 PSAPHH432 pKa = 5.35 FQASIVRR439 pKa = 11.84 MDD441 pKa = 3.6 RR442 pKa = 11.84 GLPIQHH448 pKa = 6.95
Molecular weight: 47.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.414
IPC_protein 10.511
Toseland 10.262
ProMoST 10.233
Dawson 10.467
Bjellqvist 10.335
Wikipedia 10.774
Rodwell 10.379
Grimsley 10.555
Solomon 10.613
Lehninger 10.555
Nozaki 10.394
DTASelect 10.292
Thurlkill 10.35
EMBOSS 10.701
Sillero 10.438
Patrickios 9.838
IPC_peptide 10.599
IPC2_peptide 9.838
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
37272
69
2548
392.3
43.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.722 ± 0.188
2.578 ± 0.167
4.668 ± 0.16
4.934 ± 0.173
4.373 ± 0.158
6.359 ± 0.362
2.715 ± 0.09
4.832 ± 0.18
4.014 ± 0.187
9.943 ± 0.239
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.066 ± 0.106
4.116 ± 0.16
6.45 ± 0.278
3.689 ± 0.164
6.08 ± 0.208
7.268 ± 0.182
6.581 ± 0.284
7.247 ± 0.219
1.256 ± 0.099
3.11 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here