Streptomyces sp. M41(2017)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V9KDT7|A0A1V9KDT7_9ACTN Uncharacterized protein OS=Streptomyces sp. M41(2017) OX=1955065 GN=B0675_11870 PE=4 SV=1
MM1 pKa = 7.12STPAIPVLSEE11 pKa = 4.55LDD13 pKa = 4.62DD14 pKa = 4.08IPPLSFTTPEE24 pKa = 4.42GFFSLPLAATPDD36 pKa = 3.36DD37 pKa = 3.68RR38 pKa = 11.84AALSEE43 pKa = 4.35SFVRR47 pKa = 11.84EE48 pKa = 3.94LYY50 pKa = 10.77SRR52 pKa = 11.84GDD54 pKa = 3.38EE55 pKa = 4.49ALWTPAAPYY64 pKa = 9.52YY65 pKa = 10.72AALAEE70 pKa = 4.26QLAHH74 pKa = 6.22TGVSYY79 pKa = 11.16SAMGLFSTDD88 pKa = 2.86DD89 pKa = 3.73GGVVQCALTVAAVEE103 pKa = 4.47TEE105 pKa = 3.75QDD107 pKa = 3.54DD108 pKa = 4.42PEE110 pKa = 4.82IAAHH114 pKa = 7.17GILTALSSDD123 pKa = 3.46PHH125 pKa = 7.76RR126 pKa = 11.84DD127 pKa = 3.62ARR129 pKa = 11.84WLDD132 pKa = 4.17LPCGPAVSCVTMRR145 pKa = 11.84EE146 pKa = 3.88VTLAPEE152 pKa = 4.45VTASGAEE159 pKa = 4.11VQLLTGQIQVQVPFPTGPYY178 pKa = 8.31TAVFTLDD185 pKa = 3.52TASTEE190 pKa = 4.17YY191 pKa = 9.93WGEE194 pKa = 3.91FCDD197 pKa = 5.55LLIAVLRR204 pKa = 11.84TLSFRR209 pKa = 11.84DD210 pKa = 3.6TAPDD214 pKa = 3.18ISDD217 pKa = 3.54AASS220 pKa = 2.89

Molecular weight: 23.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V9KCL0|A0A1V9KCL0_9ACTN Molybdopterin molybdenumtransferase OS=Streptomyces sp. M41(2017) OX=1955065 GN=B0675_09515 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7701

0

7701

2489732

29

7416

323.3

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.492 ± 0.04

0.759 ± 0.008

6.14 ± 0.022

5.522 ± 0.031

2.745 ± 0.016

9.569 ± 0.026

2.298 ± 0.015

3.146 ± 0.019

2.201 ± 0.021

10.231 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.722 ± 0.011

1.777 ± 0.014

6.023 ± 0.027

2.742 ± 0.013

7.947 ± 0.033

5.256 ± 0.023

6.282 ± 0.026

8.546 ± 0.024

1.517 ± 0.013

2.085 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski