Leucosporidium creatinivorum
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9828 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2FYE4|A0A1Y2FYE4_9BASI Enoyl-CoA hydratase OS=Leucosporidium creatinivorum OX=106004 GN=BCR35DRAFT_300833 PE=3 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.61 SFLALAALVPLAAAHH17 pKa = 5.71 FTLDD21 pKa = 3.45 YY22 pKa = 9.87 PATRR26 pKa = 11.84 GFDD29 pKa = 3.51 EE30 pKa = 4.98 DD31 pKa = 5.81 LEE33 pKa = 4.33 PQFCGGFPDD42 pKa = 4.05 VSSTRR47 pKa = 11.84 QPFPLSGSAPILIDD61 pKa = 3.16 SHH63 pKa = 7.35 HH64 pKa = 7.4 PSAQVGILISLDD76 pKa = 3.72 SNPTSFDD83 pKa = 3.14 NFTTTSTGDD92 pKa = 4.63 AIPMLLPFGQITGTGEE108 pKa = 3.57 FCFNVDD114 pKa = 3.31 VGSLGLSNVTNGTVATIQVEE134 pKa = 4.39 FNGGDD139 pKa = 3.78 GLLLQCTDD147 pKa = 2.98 IVLVSDD153 pKa = 3.52 YY154 pKa = 9.76 TVPSNVTCANATATTSASDD173 pKa = 3.72 SASSSATATGSMGSGTSTPASGANKK198 pKa = 9.61 VGAAVGAVLAVVGAGVLMLL217 pKa = 4.89
Molecular weight: 21.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.961
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A1Y2F0I0|A0A1Y2F0I0_9BASI DASH complex subunit DAM1 OS=Leucosporidium creatinivorum OX=106004 GN=BCR35DRAFT_305502 PE=3 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 FRR4 pKa = 11.84 RR5 pKa = 11.84 LSRR8 pKa = 11.84 LRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 GRR15 pKa = 11.84 LSRR18 pKa = 11.84 TPRR21 pKa = 11.84 PGKK24 pKa = 9.5 RR25 pKa = 11.84 RR26 pKa = 11.84 HH27 pKa = 5.72 RR28 pKa = 11.84 SSRR31 pKa = 11.84 SKK33 pKa = 10.84 SKK35 pKa = 10.44 LRR37 pKa = 11.84 RR38 pKa = 11.84 SRR40 pKa = 11.84 SRR42 pKa = 11.84 RR43 pKa = 11.84 SRR45 pKa = 11.84 LRR47 pKa = 11.84 RR48 pKa = 11.84 PLSPSSPRR56 pKa = 11.84 SPSSRR61 pKa = 11.84 RR62 pKa = 11.84 NPLSRR67 pKa = 11.84 NRR69 pKa = 11.84 SLPPLATLAPPRR81 pKa = 11.84 PPLPRR86 pKa = 11.84 RR87 pKa = 11.84 PPLGRR92 pKa = 11.84 PPKK95 pKa = 9.35 PSPSRR100 pKa = 11.84 SRR102 pKa = 11.84 RR103 pKa = 11.84 SPPTPSRR110 pKa = 11.84 RR111 pKa = 11.84 PLPLPSAPLLPSRR124 pKa = 11.84 LAVRR128 pKa = 11.84 LARR131 pKa = 11.84 LVLPRR136 pKa = 11.84 PSTRR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 MLMRR146 pKa = 11.84 RR147 pKa = 11.84 PLLRR151 pKa = 11.84 LVVVEE156 pKa = 4.49 RR157 pKa = 11.84 PRR159 pKa = 11.84 RR160 pKa = 11.84 SSPPLSRR167 pKa = 11.84 RR168 pKa = 11.84 SRR170 pKa = 11.84 LRR172 pKa = 11.84 RR173 pKa = 11.84 SPKK176 pKa = 8.61 RR177 pKa = 11.84 RR178 pKa = 11.84 RR179 pKa = 11.84 RR180 pKa = 11.84 LRR182 pKa = 11.84 RR183 pKa = 11.84 RR184 pKa = 11.84 RR185 pKa = 11.84 SSSLSLLAVARR196 pKa = 11.84 RR197 pKa = 11.84 RR198 pKa = 11.84 PRR200 pKa = 11.84 RR201 pKa = 11.84 SLRR204 pKa = 11.84 RR205 pKa = 11.84 RR206 pKa = 11.84 PPSPSAGARR215 pKa = 11.84 WSLSSRR221 pKa = 11.84 SSSLPFAVAEE231 pKa = 4.13 PSPPRR236 pKa = 11.84 RR237 pKa = 11.84 PPRR240 pKa = 11.84 SSLRR244 pKa = 11.84 TSLKK248 pKa = 10.01 LRR250 pKa = 11.84 SIRR253 pKa = 11.84 SRR255 pKa = 11.84 RR256 pKa = 11.84 SPPPSRR262 pKa = 11.84 RR263 pKa = 11.84 PLPLARR269 pKa = 11.84 DD270 pKa = 4.04 VPPKK274 pKa = 10.48 LPSPFSRR281 pKa = 11.84 TRR283 pKa = 11.84 RR284 pKa = 11.84 IWTTSPPPLPPLALLEE300 pKa = 4.28 ASAPSSSSRR309 pKa = 11.84 ARR311 pKa = 11.84 RR312 pKa = 11.84 MLLPPPPLPPRR323 pKa = 11.84 PPGQLAPRR331 pKa = 11.84 RR332 pKa = 11.84 SSRR335 pKa = 11.84 RR336 pKa = 11.84 TQRR339 pKa = 11.84 PPLPSRR345 pKa = 11.84 GEE347 pKa = 3.89 HH348 pKa = 6.62 PPRR351 pKa = 11.84 RR352 pKa = 11.84 WW353 pKa = 2.91
Molecular weight: 40.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.518
IPC2_protein 11.418
IPC_protein 13.027
Toseland 13.188
ProMoST 13.685
Dawson 13.188
Bjellqvist 13.188
Wikipedia 13.671
Rodwell 12.72
Grimsley 13.232
Solomon 13.685
Lehninger 13.598
Nozaki 13.188
DTASelect 13.188
Thurlkill 13.188
EMBOSS 13.685
Sillero 13.188
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.676
IPC2.peptide.svr19 9.325
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9828
0
9828
4540455
49
5392
462.0
50.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.81 ± 0.023
1.067 ± 0.011
5.052 ± 0.018
6.543 ± 0.022
3.555 ± 0.014
7.258 ± 0.022
2.193 ± 0.01
4.022 ± 0.017
4.445 ± 0.022
9.796 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.762 ± 0.009
2.701 ± 0.012
6.909 ± 0.036
3.495 ± 0.016
6.288 ± 0.023
9.407 ± 0.041
5.968 ± 0.019
6.023 ± 0.017
1.386 ± 0.009
2.319 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here