Nocardia otitidiscaviarum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6808 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A379JNI6|A0A379JNI6_9NOCA Type IV secretory pathway VirB4 components OS=Nocardia otitidiscaviarum OX=1823 GN=NCTC1934_06937 PE=4 SV=1
MM1 pKa = 7.72GFPEE5 pKa = 4.26HH6 pKa = 6.86LSYY9 pKa = 11.11TEE11 pKa = 3.73EE12 pKa = 4.43HH13 pKa = 6.32EE14 pKa = 4.27WLHH17 pKa = 6.1VVDD20 pKa = 5.04GTATVGVTDD29 pKa = 4.86FAASSLGDD37 pKa = 3.43VVFVQLPAVGDD48 pKa = 3.65RR49 pKa = 11.84VAQGEE54 pKa = 4.5VCGEE58 pKa = 3.91IEE60 pKa = 4.3STKK63 pKa = 10.64SVSDD67 pKa = 4.05LYY69 pKa = 11.58SPVTGEE75 pKa = 4.01VVEE78 pKa = 4.5INAAVVDD85 pKa = 5.0DD86 pKa = 4.86PDD88 pKa = 4.14TVNSDD93 pKa = 3.4PYY95 pKa = 9.63GAGWLFRR102 pKa = 11.84VRR104 pKa = 11.84VEE106 pKa = 4.1STGALLDD113 pKa = 3.58ATAYY117 pKa = 10.21AALTGDD123 pKa = 3.55

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A378Y795|A0A378Y795_9NOCA 2-acyl-glycerophospho-ethanolamine acyltransferase OS=Nocardia otitidiscaviarum OX=1823 GN=NCTC1934_00157 PE=4 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84TVPTRR7 pKa = 11.84PATTVAAWPPATAASRR23 pKa = 11.84RR24 pKa = 11.84TTTVVTRR31 pKa = 11.84LPAAVATGPLTAAATRR47 pKa = 11.84PPAAVTRR54 pKa = 11.84LSVASPTRR62 pKa = 11.84PHH64 pKa = 6.31SAGAAWGSPVGGSRR78 pKa = 11.84RR79 pKa = 11.84GSGARR84 pKa = 3.42

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6808

0

6808

2213753

29

14775

325.2

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.646 ± 0.045

0.745 ± 0.011

6.256 ± 0.033

5.496 ± 0.024

2.895 ± 0.019

8.899 ± 0.034

2.237 ± 0.013

3.946 ± 0.02

1.756 ± 0.022

10.16 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.014

1.885 ± 0.016

6.064 ± 0.029

2.632 ± 0.018

8.116 ± 0.035

4.956 ± 0.019

6.334 ± 0.023

8.537 ± 0.031

1.478 ± 0.012

2.078 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski