thiotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2032 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A483B0H9|A0A483B0H9_9BACT Uncharacterized protein (Fragment) OS=thiotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev) OX=343240 GN=BPUTEOSOX_954 PE=4 SV=1
SS1 pKa = 5.95NTTFQNYY8 pKa = 10.19DD9 pKa = 3.84DD10 pKa = 3.84IVEE13 pKa = 4.56RR14 pKa = 11.84SCQAWNEE21 pKa = 3.96ILSEE25 pKa = 4.47DD26 pKa = 3.85GFIEE30 pKa = 4.12NLCSRR35 pKa = 11.84EE36 pKa = 3.53WSFFGLVVFF45 pKa = 5.57

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A483AKC7|A0A483AKC7_9BACT Cytochrome c domain-containing protein OS=thiotrophic endosymbiont of Bathymodiolus puteoserpentis (Logatchev) OX=343240 GN=BPUTEOSOX_1363 PE=4 SV=1
MM1 pKa = 7.26VKK3 pKa = 10.32LRR5 pKa = 11.84LLFDD9 pKa = 5.61CINIMKK15 pKa = 10.01QKK17 pKa = 9.61RR18 pKa = 11.84TFQPSVIKK26 pKa = 10.52RR27 pKa = 11.84KK28 pKa = 8.26RR29 pKa = 11.84THH31 pKa = 5.93GFRR34 pKa = 11.84ARR36 pKa = 11.84MSTKK40 pKa = 10.28AGRR43 pKa = 11.84AVINARR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 8.85GRR54 pKa = 11.84KK55 pKa = 8.82RR56 pKa = 11.84LAAA59 pKa = 4.42

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2032

0

2032

598202

21

2919

294.4

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.152 ± 0.06

0.964 ± 0.021

5.83 ± 0.048

5.624 ± 0.055

4.247 ± 0.043

6.584 ± 0.075

2.149 ± 0.023

7.778 ± 0.048

7.618 ± 0.057

9.588 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.03

5.831 ± 0.064

3.17 ± 0.036

4.189 ± 0.043

3.694 ± 0.039

6.743 ± 0.047

5.771 ± 0.057

6.333 ± 0.05

1.015 ± 0.019

3.344 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski