Pectobacterium phage Q19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pektosvirus; unclassified Pektosvirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A678ZKE0|A0A678ZKE0_9CAUD DNA primase/helicase OS=Pectobacterium phage Q19 OX=2500576 GN=Q19_21 PE=4 SV=1
MM1 pKa = 7.43IAFMSDD7 pKa = 3.0PANNVWVFLTVFMVTYY23 pKa = 9.74WLLEE27 pKa = 4.28GDD29 pKa = 4.05DD30 pKa = 4.68HH31 pKa = 8.88DD32 pKa = 5.69PII34 pKa = 6.22

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A678ZZL9|A0A678ZZL9_9CAUD Uncharacterized protein OS=Pectobacterium phage Q19 OX=2500576 GN=Q19_14 PE=4 SV=1
MM1 pKa = 7.57LAPYY5 pKa = 9.34GATVRR10 pKa = 11.84TMKK13 pKa = 10.21RR14 pKa = 11.84RR15 pKa = 11.84CVALGLPSRR24 pKa = 11.84AAKK27 pKa = 10.37ALAKK31 pKa = 9.88HH32 pKa = 5.5HH33 pKa = 6.93HH34 pKa = 6.01NLVTKK39 pKa = 10.53AQADD43 pKa = 3.2WMYY46 pKa = 11.65

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12213

34

1331

244.3

27.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.023 ± 0.414

0.909 ± 0.171

6.436 ± 0.233

6.297 ± 0.307

3.75 ± 0.2

7.795 ± 0.327

1.883 ± 0.183

5.036 ± 0.158

6.198 ± 0.367

8.237 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.158

4.553 ± 0.194

3.709 ± 0.158

4.094 ± 0.346

5.306 ± 0.231

6.182 ± 0.286

6.035 ± 0.289

7.115 ± 0.339

1.433 ± 0.162

3.292 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski