Komagataeibacter hansenii ATCC 23769
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3303 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5QB18|D5QB18_KOMHA tRNA pseudouridine synthase B OS=Komagataeibacter hansenii ATCC 23769 OX=714995 GN=truB PE=3 SV=1
MM1 pKa = 7.4 PQLSLHH7 pKa = 6.39 SPTGPLTVSAEE18 pKa = 4.06 DD19 pKa = 3.71 DD20 pKa = 4.09 TIVALDD26 pKa = 3.64 WGWGRR31 pKa = 11.84 DD32 pKa = 3.7 QEE34 pKa = 4.43 EE35 pKa = 4.52 TPLLRR40 pKa = 11.84 RR41 pKa = 11.84 ACDD44 pKa = 3.0 WLDD47 pKa = 3.17 AYY49 pKa = 11.07 FDD51 pKa = 4.63 GDD53 pKa = 4.02 TTPCTLPLSPYY64 pKa = 7.78 GTPQQVAAWTFIRR77 pKa = 11.84 TIPMGHH83 pKa = 6.45 TLPCAEE89 pKa = 4.73 VARR92 pKa = 11.84 QASVTVADD100 pKa = 3.98 VVAACSEE107 pKa = 4.1 NPIPILIPCHH117 pKa = 5.4 RR118 pKa = 11.84 VNMEE122 pKa = 3.37 GCADD126 pKa = 3.99 YY127 pKa = 11.2 DD128 pKa = 3.94 ADD130 pKa = 4.41 TYY132 pKa = 11.2 PGDD135 pKa = 3.6 RR136 pKa = 11.84 GAMDD140 pKa = 3.67 RR141 pKa = 11.84 AFLRR145 pKa = 11.84 DD146 pKa = 4.15 LEE148 pKa = 4.36 TDD150 pKa = 4.02 APDD153 pKa = 4.03 LDD155 pKa = 3.98
Molecular weight: 16.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.986
IPC_protein 3.986
Toseland 3.757
ProMoST 4.101
Dawson 3.986
Bjellqvist 4.177
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.368
Thurlkill 3.821
EMBOSS 3.948
Sillero 4.101
Patrickios 1.952
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|D5QAY4|D5QAY4_KOMHA Uncharacterized protein OS=Komagataeibacter hansenii ATCC 23769 OX=714995 GN=GXY_01288 PE=4 SV=1
MM1 pKa = 7.31 TVVQLLSPFMLALSVGVAAALHH23 pKa = 5.77 LVRR26 pKa = 11.84 ARR28 pKa = 11.84 GLAPQAQPVRR38 pKa = 11.84 VRR40 pKa = 11.84 NRR42 pKa = 11.84 RR43 pKa = 3.25
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3303
0
3303
1001832
21
3523
303.3
32.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.924 ± 0.058
1.111 ± 0.015
5.729 ± 0.036
4.714 ± 0.041
3.406 ± 0.028
8.675 ± 0.044
2.739 ± 0.024
5.106 ± 0.035
2.346 ± 0.028
9.85 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.032 ± 0.024
2.613 ± 0.034
5.663 ± 0.034
3.409 ± 0.026
7.496 ± 0.052
5.395 ± 0.035
5.762 ± 0.04
7.358 ± 0.044
1.416 ± 0.017
2.253 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here