Natronobacterium gregoryi (strain ATCC 43098 / DSM 3393 / CCM 3738 / CIP 104747 / IAM 13177 / JCM 8860 / NBRC 102187 / NCIMB 2189 / SP2)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natronobacterium; Natronobacterium gregoryi

Average proteome isoelectric point is 4.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3624 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0AKB0|L0AKB0_NATGS ABC-type polar amino acid transport system ATPase component OS=Natronobacterium gregoryi (strain ATCC 43098 / DSM 3393 / CCM 3738 / CIP 104747 / IAM 13177 / JCM 8860 / NBRC 102187 / NCIMB 2189 / SP2) OX=797304 GN=Natgr_3203 PE=4 SV=1
MM1 pKa = 7.85ADD3 pKa = 4.17ALDD6 pKa = 4.18DD7 pKa = 5.97DD8 pKa = 4.6IYY10 pKa = 11.48QRR12 pKa = 11.84TKK14 pKa = 11.12ALLEE18 pKa = 4.39PGEE21 pKa = 4.25IKK23 pKa = 11.07LNGAVVHH30 pKa = 5.86TEE32 pKa = 3.62YY33 pKa = 11.18DD34 pKa = 3.22GGDD37 pKa = 3.85DD38 pKa = 3.74VKK40 pKa = 11.01MMQATIDD47 pKa = 3.66VGDD50 pKa = 4.23VIAAHH55 pKa = 6.52SGHH58 pKa = 7.2DD59 pKa = 3.65PNDD62 pKa = 3.68CYY64 pKa = 11.34VYY66 pKa = 10.73SGNDD70 pKa = 3.42DD71 pKa = 4.02PDD73 pKa = 3.96FSSNQHH79 pKa = 5.89QGLTLDD85 pKa = 4.21DD86 pKa = 4.98EE87 pKa = 4.67EE88 pKa = 5.5FVWEE92 pKa = 4.34CQQLLRR98 pKa = 11.84DD99 pKa = 3.72GSFDD103 pKa = 2.96IVIYY107 pKa = 10.33YY108 pKa = 9.4RR109 pKa = 11.84ASADD113 pKa = 2.96HH114 pKa = 6.71GAILDD119 pKa = 4.43EE120 pKa = 4.24IQEE123 pKa = 4.27LGFDD127 pKa = 3.62VTGVEE132 pKa = 4.95GEE134 pKa = 4.07

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4GCB0|G4GCB0_NATGS Cupin OS=Natronobacterium gregoryi (strain ATCC 43098 / DSM 3393 / CCM 3738 / CIP 104747 / IAM 13177 / JCM 8860 / NBRC 102187 / NCIMB 2189 / SP2) OX=797304 GN=Natgr_0271 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 4.87RR3 pKa = 11.84WQRR6 pKa = 11.84RR7 pKa = 11.84AVKK10 pKa = 8.78TITGIVVLVFLTSLAYY26 pKa = 9.66HH27 pKa = 5.82YY28 pKa = 11.56VMIVFEE34 pKa = 4.86GRR36 pKa = 11.84SSSYY40 pKa = 9.67FHH42 pKa = 6.25STQVVVEE49 pKa = 4.26TFPGTGYY56 pKa = 10.99GSDD59 pKa = 4.3SPWEE63 pKa = 4.13SAVGNLFVIVMGLSTFCSCLSFSRR87 pKa = 11.84TSSGPSSSGRR97 pKa = 11.84SRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84YY102 pKa = 8.46RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84SSRR109 pKa = 11.84ITSWCVATAPASNDD123 pKa = 3.34SASEE127 pKa = 4.03TKK129 pKa = 10.4ARR131 pKa = 11.84QAGADD136 pKa = 3.82YY137 pKa = 10.99VLSLPDD143 pKa = 2.99ISGRR147 pKa = 11.84LLALDD152 pKa = 3.76VLRR155 pKa = 11.84DD156 pKa = 3.5ASISYY161 pKa = 9.99DD162 pKa = 3.34RR163 pKa = 11.84QLKK166 pKa = 9.36IVRR169 pKa = 11.84FEE171 pKa = 4.41ADD173 pKa = 3.5PLTGRR178 pKa = 11.84AVTNTPLPEE187 pKa = 4.47LNCTLIAVEE196 pKa = 4.73RR197 pKa = 11.84SDD199 pKa = 5.3EE200 pKa = 4.21NVTDD204 pKa = 4.47PAPSPISGAATTCCSPVTTTRR225 pKa = 11.84STRR228 pKa = 11.84SNQNSSKK235 pKa = 10.09FSSVVAATVRR245 pKa = 11.84WRR247 pKa = 11.84KK248 pKa = 8.91YY249 pKa = 8.86RR250 pKa = 11.84RR251 pKa = 11.84CSSSVLTAVFATRR264 pKa = 11.84HH265 pKa = 4.81YY266 pKa = 11.03RR267 pKa = 11.84NDD269 pKa = 3.37LTVHH273 pKa = 6.48LGVTPSEE280 pKa = 4.1NLRR283 pKa = 11.84FAWVLEE289 pKa = 4.31TNDD292 pKa = 2.93FRR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84CGFRR300 pKa = 11.84VVGPASITRR309 pKa = 11.84SAVAQLGRR317 pKa = 11.84SSLLSARR324 pKa = 11.84SVRR327 pKa = 11.84LVPSQAA333 pKa = 3.25

Molecular weight: 36.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3624

0

3624

1028101

29

1681

283.7

31.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.17 ± 0.053

0.783 ± 0.014

8.716 ± 0.056

9.587 ± 0.062

3.228 ± 0.031

8.068 ± 0.042

1.987 ± 0.018

4.24 ± 0.036

1.859 ± 0.024

8.932 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.68 ± 0.02

2.254 ± 0.019

4.539 ± 0.029

2.554 ± 0.026

6.41 ± 0.042

5.552 ± 0.03

6.493 ± 0.035

9.098 ± 0.041

1.113 ± 0.015

2.734 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski