Sewage-associated circular DNA virus-15

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGX1|A0A0B4UGX1_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-15 OX=1592082 PE=4 SV=1
MM1 pKa = 7.91GDD3 pKa = 3.16TSQRR7 pKa = 11.84ATCWSITINNPLPSDD22 pKa = 3.51IPTAEE27 pKa = 4.35ALPAKK32 pKa = 9.35WVLQGQLEE40 pKa = 4.23EE41 pKa = 4.47GKK43 pKa = 10.15EE44 pKa = 4.37GTVHH48 pKa = 4.86YY49 pKa = 10.34QGMLTTPQCRR59 pKa = 11.84FSQVKK64 pKa = 9.65KK65 pKa = 9.63VLPRR69 pKa = 11.84AHH71 pKa = 6.92IEE73 pKa = 3.89VAKK76 pKa = 10.89NRR78 pKa = 11.84TALEE82 pKa = 4.32KK83 pKa = 10.63YY84 pKa = 7.4VHH86 pKa = 6.76KK87 pKa = 10.55EE88 pKa = 3.4DD89 pKa = 3.58TRR91 pKa = 11.84LVEE94 pKa = 4.23VPTVSSSLPTLFDD107 pKa = 3.36YY108 pKa = 11.18QFTVAGRR115 pKa = 11.84WVSTEE120 pKa = 3.31FDD122 pKa = 3.47KK123 pKa = 11.87YY124 pKa = 11.02IDD126 pKa = 4.03DD127 pKa = 5.65FKK129 pKa = 11.64DD130 pKa = 3.27KK131 pKa = 11.06DD132 pKa = 3.54IGDD135 pKa = 3.75VALLYY140 pKa = 10.65VDD142 pKa = 4.57SLVEE146 pKa = 3.76KK147 pKa = 10.61DD148 pKa = 4.24IINGMRR154 pKa = 11.84GIEE157 pKa = 4.41FIAINPMWRR166 pKa = 11.84SSWKK170 pKa = 10.1RR171 pKa = 11.84FWRR174 pKa = 11.84GIILRR179 pKa = 11.84HH180 pKa = 6.09RR181 pKa = 11.84SIEE184 pKa = 4.11DD185 pKa = 3.09AQARR189 pKa = 11.84QEE191 pKa = 4.82DD192 pKa = 4.16IPSQEE197 pKa = 4.09EE198 pKa = 4.49CTASPCSEE206 pKa = 4.03AQDD209 pKa = 4.49GEE211 pKa = 4.72CEE213 pKa = 3.89

Molecular weight: 24.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGX1|A0A0B4UGX1_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-15 OX=1592082 PE=4 SV=1
MM1 pKa = 7.69PKK3 pKa = 10.0LVKK6 pKa = 9.19KK7 pKa = 8.82TYY9 pKa = 9.21PRR11 pKa = 11.84KK12 pKa = 9.17KK13 pKa = 7.9NVRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84VAKK22 pKa = 10.25HH23 pKa = 4.2RR24 pKa = 11.84TVNVNRR30 pKa = 11.84ALQPFAQRR38 pKa = 11.84YY39 pKa = 4.58ITKK42 pKa = 9.03MKK44 pKa = 10.47YY45 pKa = 10.0SEE47 pKa = 5.49AYY49 pKa = 8.3TLNAGNIWSQIMNLNSIFDD68 pKa = 4.3PNRR71 pKa = 11.84TGIGHH76 pKa = 5.8QPYY79 pKa = 10.47GHH81 pKa = 7.17DD82 pKa = 3.84TLQQIYY88 pKa = 10.17GRR90 pKa = 11.84YY91 pKa = 8.84RR92 pKa = 11.84VISCKK97 pKa = 10.58YY98 pKa = 10.0VVNCYY103 pKa = 10.28NATTPIRR110 pKa = 11.84FGCLPANEE118 pKa = 4.69IPPVSTVSEE127 pKa = 4.18LCEE130 pKa = 3.87NPRR133 pKa = 11.84SQFRR137 pKa = 11.84IQFPGGSTQTIAGVVSIPSLVGRR160 pKa = 11.84NKK162 pKa = 9.78SQYY165 pKa = 8.71MADD168 pKa = 4.44DD169 pKa = 4.59RR170 pKa = 11.84FQAPFGSSPAEE181 pKa = 3.7LALLYY186 pKa = 9.57ITGQSMADD194 pKa = 3.42SNVDD198 pKa = 2.77INLNVTLEE206 pKa = 3.96YY207 pKa = 10.59LVEE210 pKa = 4.41CFDD213 pKa = 5.32VIPLEE218 pKa = 4.24QSS220 pKa = 2.86

Molecular weight: 24.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

433

213

220

216.5

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.236 ± 0.236

2.309 ± 0.027

5.081 ± 1.37

6.005 ± 1.709

3.464 ± 0.124

5.312 ± 0.103

1.617 ± 0.183

6.928 ± 0.08

5.081 ± 0.386

6.697 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.14

4.85 ± 1.748

6.005 ± 0.587

5.543 ± 0.264

6.697 ± 0.415

7.159 ± 0.082

6.236 ± 0.236

7.159 ± 0.41

1.617 ± 0.839

3.926 ± 1.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski