Amino acid dipepetide frequency for Proteus phage VB_PmiS-Isfahan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.812AlaAla: 1.812 ± 0.562
0.604AlaCys: 0.604 ± 0.172
3.806AlaAsp: 3.806 ± 0.453
5.86AlaGlu: 5.86 ± 0.785
2.235AlaPhe: 2.235 ± 0.367
4.048AlaGly: 4.048 ± 0.579
1.269AlaHis: 1.269 ± 0.302
4.108AlaIle: 4.108 ± 0.584
4.893AlaLys: 4.893 ± 0.766
5.437AlaLeu: 5.437 ± 0.47
3.081AlaMet: 3.081 ± 0.486
3.866AlaAsn: 3.866 ± 0.993
1.812AlaPro: 1.812 ± 0.317
2.356AlaGln: 2.356 ± 0.472
2.175AlaArg: 2.175 ± 0.354
4.108AlaSer: 4.108 ± 0.684
3.081AlaThr: 3.081 ± 0.463
4.833AlaVal: 4.833 ± 0.478
0.665AlaTrp: 0.665 ± 0.165
1.812AlaTyr: 1.812 ± 0.252
0.0AlaXaa: 0.0 ± 0.0
Cys
0.483CysAla: 0.483 ± 0.174
0.483CysCys: 0.483 ± 0.177
1.087CysAsp: 1.087 ± 0.285
1.692CysGlu: 1.692 ± 0.329
0.544CysPhe: 0.544 ± 0.211
1.087CysGly: 1.087 ± 0.297
0.06CysHis: 0.06 ± 0.06
0.725CysIle: 0.725 ± 0.249
1.631CysLys: 1.631 ± 0.393
1.329CysLeu: 1.329 ± 0.252
0.483CysMet: 0.483 ± 0.149
0.846CysAsn: 0.846 ± 0.212
0.362CysPro: 0.362 ± 0.17
0.362CysGln: 0.362 ± 0.142
0.604CysArg: 0.604 ± 0.195
0.665CysSer: 0.665 ± 0.192
0.846CysThr: 0.846 ± 0.261
0.725CysVal: 0.725 ± 0.21
0.181CysTrp: 0.181 ± 0.095
0.785CysTyr: 0.785 ± 0.251
0.0CysXaa: 0.0 ± 0.0
Asp
4.35AspAla: 4.35 ± 0.44
1.027AspCys: 1.027 ± 0.228
4.168AspAsp: 4.168 ± 0.481
3.987AspGlu: 3.987 ± 0.365
1.873AspPhe: 1.873 ± 0.365
5.8AspGly: 5.8 ± 0.737
0.846AspHis: 0.846 ± 0.237
4.168AspIle: 4.168 ± 0.477
4.712AspLys: 4.712 ± 0.532
4.168AspLeu: 4.168 ± 0.606
1.692AspMet: 1.692 ± 0.286
4.893AspAsn: 4.893 ± 0.611
2.356AspPro: 2.356 ± 0.376
1.994AspGln: 1.994 ± 0.315
2.477AspArg: 2.477 ± 0.43
3.202AspSer: 3.202 ± 0.356
2.96AspThr: 2.96 ± 0.461
4.41AspVal: 4.41 ± 0.41
0.906AspTrp: 0.906 ± 0.235
3.323AspTyr: 3.323 ± 0.485
0.0AspXaa: 0.0 ± 0.0
Glu
4.954GluAla: 4.954 ± 0.677
1.51GluCys: 1.51 ± 0.277
2.96GluAsp: 2.96 ± 0.367
4.229GluGlu: 4.229 ± 0.41
2.598GluPhe: 2.598 ± 0.429
3.504GluGly: 3.504 ± 0.435
1.148GluHis: 1.148 ± 0.29
5.075GluIle: 5.075 ± 0.519
5.195GluLys: 5.195 ± 0.6
7.914GluLeu: 7.914 ± 0.82
2.114GluMet: 2.114 ± 0.422
3.443GluAsn: 3.443 ± 0.396
2.839GluPro: 2.839 ± 0.44
2.96GluGln: 2.96 ± 0.489
4.048GluArg: 4.048 ± 0.553
3.806GluSer: 3.806 ± 0.57
4.47GluThr: 4.47 ± 0.575
2.9GluVal: 2.9 ± 0.477
1.148GluTrp: 1.148 ± 0.286
3.081GluTyr: 3.081 ± 0.392
0.0GluXaa: 0.0 ± 0.0
Phe
1.812PheAla: 1.812 ± 0.479
0.423PheCys: 0.423 ± 0.144
2.719PheAsp: 2.719 ± 0.416
2.779PheGlu: 2.779 ± 0.351
1.087PhePhe: 1.087 ± 0.293
2.537PheGly: 2.537 ± 0.406
0.544PheHis: 0.544 ± 0.169
3.685PheIle: 3.685 ± 0.557
3.866PheLys: 3.866 ± 0.493
2.416PheLeu: 2.416 ± 0.343
0.846PheMet: 0.846 ± 0.237
3.081PheAsn: 3.081 ± 0.44
1.51PhePro: 1.51 ± 0.315
1.027PheGln: 1.027 ± 0.197
1.087PheArg: 1.087 ± 0.249
2.356PheSer: 2.356 ± 0.326
2.416PheThr: 2.416 ± 0.485
2.658PheVal: 2.658 ± 0.423
0.362PheTrp: 0.362 ± 0.133
1.812PheTyr: 1.812 ± 0.367
0.0PheXaa: 0.0 ± 0.0
Gly
3.806GlyAla: 3.806 ± 0.481
1.027GlyCys: 1.027 ± 0.271
4.773GlyAsp: 4.773 ± 0.542
4.773GlyGlu: 4.773 ± 0.505
4.229GlyPhe: 4.229 ± 0.626
3.202GlyGly: 3.202 ± 0.524
1.45GlyHis: 1.45 ± 0.33
4.168GlyIle: 4.168 ± 0.613
5.075GlyLys: 5.075 ± 0.6
4.712GlyLeu: 4.712 ± 0.604
1.631GlyMet: 1.631 ± 0.303
3.685GlyAsn: 3.685 ± 0.432
1.148GlyPro: 1.148 ± 0.238
2.296GlyGln: 2.296 ± 0.386
3.323GlyArg: 3.323 ± 0.42
3.987GlySer: 3.987 ± 0.605
3.081GlyThr: 3.081 ± 0.422
5.256GlyVal: 5.256 ± 0.551
1.087GlyTrp: 1.087 ± 0.243
2.054GlyTyr: 2.054 ± 0.321
0.0GlyXaa: 0.0 ± 0.0
His
0.785HisAla: 0.785 ± 0.215
0.302HisCys: 0.302 ± 0.12
1.389HisAsp: 1.389 ± 0.291
0.785HisGlu: 0.785 ± 0.266
1.027HisPhe: 1.027 ± 0.267
1.208HisGly: 1.208 ± 0.37
0.483HisHis: 0.483 ± 0.18
1.148HisIle: 1.148 ± 0.283
1.027HisLys: 1.027 ± 0.261
1.571HisLeu: 1.571 ± 0.319
0.362HisMet: 0.362 ± 0.133
0.967HisAsn: 0.967 ± 0.236
0.785HisPro: 0.785 ± 0.199
0.665HisGln: 0.665 ± 0.215
0.906HisArg: 0.906 ± 0.221
1.208HisSer: 1.208 ± 0.285
0.665HisThr: 0.665 ± 0.197
1.51HisVal: 1.51 ± 0.309
0.362HisTrp: 0.362 ± 0.16
0.483HisTyr: 0.483 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
6.041IleAla: 6.041 ± 0.713
1.027IleCys: 1.027 ± 0.282
5.8IleAsp: 5.8 ± 0.522
4.35IleGlu: 4.35 ± 0.496
1.752IlePhe: 1.752 ± 0.332
4.289IleGly: 4.289 ± 0.556
1.329IleHis: 1.329 ± 0.265
5.014IleIle: 5.014 ± 0.554
5.86IleLys: 5.86 ± 0.614
4.773IleLeu: 4.773 ± 0.444
1.571IleMet: 1.571 ± 0.307
5.256IleAsn: 5.256 ± 0.565
2.658IlePro: 2.658 ± 0.405
1.631IleGln: 1.631 ± 0.325
3.443IleArg: 3.443 ± 0.43
4.41IleSer: 4.41 ± 0.553
5.377IleThr: 5.377 ± 0.58
3.927IleVal: 3.927 ± 0.533
0.483IleTrp: 0.483 ± 0.154
2.779IleTyr: 2.779 ± 0.452
0.0IleXaa: 0.0 ± 0.0
Lys
5.195LysAla: 5.195 ± 0.647
0.967LysCys: 0.967 ± 0.281
4.591LysAsp: 4.591 ± 0.459
4.893LysGlu: 4.893 ± 0.57
3.021LysPhe: 3.021 ± 0.395
3.021LysGly: 3.021 ± 0.412
1.389LysHis: 1.389 ± 0.364
3.866LysIle: 3.866 ± 0.451
3.262LysLys: 3.262 ± 0.412
7.37LysLeu: 7.37 ± 0.828
3.081LysMet: 3.081 ± 0.427
3.866LysAsn: 3.866 ± 0.478
3.141LysPro: 3.141 ± 0.428
2.296LysGln: 2.296 ± 0.582
3.746LysArg: 3.746 ± 0.441
4.652LysSer: 4.652 ± 0.476
3.987LysThr: 3.987 ± 0.545
4.35LysVal: 4.35 ± 0.579
1.571LysTrp: 1.571 ± 0.319
3.323LysTyr: 3.323 ± 0.527
0.0LysXaa: 0.0 ± 0.0
Leu
5.195LeuAla: 5.195 ± 0.602
1.45LeuCys: 1.45 ± 0.277
4.893LeuAsp: 4.893 ± 0.489
7.068LeuGlu: 7.068 ± 0.648
2.658LeuPhe: 2.658 ± 0.315
3.866LeuGly: 3.866 ± 0.468
1.45LeuHis: 1.45 ± 0.24
5.86LeuIle: 5.86 ± 0.698
7.249LeuLys: 7.249 ± 0.717
5.679LeuLeu: 5.679 ± 0.663
2.839LeuMet: 2.839 ± 0.461
5.86LeuAsn: 5.86 ± 0.774
3.927LeuPro: 3.927 ± 0.495
2.296LeuGln: 2.296 ± 0.442
3.685LeuArg: 3.685 ± 0.499
4.41LeuSer: 4.41 ± 0.653
5.195LeuThr: 5.195 ± 0.633
4.591LeuVal: 4.591 ± 0.618
0.665LeuTrp: 0.665 ± 0.214
2.9LeuTyr: 2.9 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
1.571MetAla: 1.571 ± 0.291
0.362MetCys: 0.362 ± 0.152
1.329MetAsp: 1.329 ± 0.252
0.785MetGlu: 0.785 ± 0.214
0.906MetPhe: 0.906 ± 0.24
1.208MetGly: 1.208 ± 0.235
0.423MetHis: 0.423 ± 0.155
2.719MetIle: 2.719 ± 0.458
2.175MetLys: 2.175 ± 0.357
2.296MetLeu: 2.296 ± 0.337
1.027MetMet: 1.027 ± 0.229
1.571MetAsn: 1.571 ± 0.299
0.785MetPro: 0.785 ± 0.214
1.269MetGln: 1.269 ± 0.257
1.631MetArg: 1.631 ± 0.26
2.477MetSer: 2.477 ± 0.328
2.416MetThr: 2.416 ± 0.329
1.692MetVal: 1.692 ± 0.325
0.604MetTrp: 0.604 ± 0.16
0.967MetTyr: 0.967 ± 0.293
0.0MetXaa: 0.0 ± 0.0
Asn
4.108AsnAla: 4.108 ± 0.413
1.148AsnCys: 1.148 ± 0.252
3.202AsnAsp: 3.202 ± 0.418
3.443AsnGlu: 3.443 ± 0.43
1.631AsnPhe: 1.631 ± 0.287
5.8AsnGly: 5.8 ± 0.697
1.087AsnHis: 1.087 ± 0.246
5.618AsnIle: 5.618 ± 0.583
4.168AsnLys: 4.168 ± 0.658
4.35AsnLeu: 4.35 ± 0.53
1.087AsnMet: 1.087 ± 0.232
4.712AsnAsn: 4.712 ± 0.51
1.994AsnPro: 1.994 ± 0.414
2.356AsnGln: 2.356 ± 0.537
3.081AsnArg: 3.081 ± 0.455
3.443AsnSer: 3.443 ± 0.557
3.443AsnThr: 3.443 ± 0.556
3.806AsnVal: 3.806 ± 0.487
0.785AsnTrp: 0.785 ± 0.248
2.658AsnTyr: 2.658 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
2.054ProAla: 2.054 ± 0.414
0.483ProCys: 0.483 ± 0.18
2.598ProAsp: 2.598 ± 0.411
2.96ProGlu: 2.96 ± 0.38
1.873ProPhe: 1.873 ± 0.36
1.994ProGly: 1.994 ± 0.313
0.423ProHis: 0.423 ± 0.128
2.175ProIle: 2.175 ± 0.36
1.631ProLys: 1.631 ± 0.382
2.356ProLeu: 2.356 ± 0.37
0.785ProMet: 0.785 ± 0.201
2.296ProAsn: 2.296 ± 0.407
0.967ProPro: 0.967 ± 0.223
0.906ProGln: 0.906 ± 0.241
1.812ProArg: 1.812 ± 0.27
1.933ProSer: 1.933 ± 0.252
2.537ProThr: 2.537 ± 0.473
2.9ProVal: 2.9 ± 0.467
0.362ProTrp: 0.362 ± 0.142
1.571ProTyr: 1.571 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
2.175GlnAla: 2.175 ± 0.47
0.665GlnCys: 0.665 ± 0.224
1.45GlnAsp: 1.45 ± 0.217
1.812GlnGlu: 1.812 ± 0.391
1.329GlnPhe: 1.329 ± 0.257
1.269GlnGly: 1.269 ± 0.278
0.423GlnHis: 0.423 ± 0.135
2.054GlnIle: 2.054 ± 0.28
1.752GlnLys: 1.752 ± 0.613
3.806GlnLeu: 3.806 ± 0.696
0.967GlnMet: 0.967 ± 0.207
1.571GlnAsn: 1.571 ± 0.373
1.027GlnPro: 1.027 ± 0.223
1.812GlnGln: 1.812 ± 0.507
1.571GlnArg: 1.571 ± 0.314
2.839GlnSer: 2.839 ± 0.396
1.571GlnThr: 1.571 ± 0.273
2.114GlnVal: 2.114 ± 0.39
0.846GlnTrp: 0.846 ± 0.167
1.51GlnTyr: 1.51 ± 0.323
0.0GlnXaa: 0.0 ± 0.0
Arg
3.262ArgAla: 3.262 ± 0.52
0.846ArgCys: 0.846 ± 0.243
3.202ArgAsp: 3.202 ± 0.526
2.719ArgGlu: 2.719 ± 0.34
1.933ArgPhe: 1.933 ± 0.273
3.021ArgGly: 3.021 ± 0.394
0.544ArgHis: 0.544 ± 0.153
3.383ArgIle: 3.383 ± 0.368
3.081ArgLys: 3.081 ± 0.326
4.41ArgLeu: 4.41 ± 0.471
1.087ArgMet: 1.087 ± 0.232
3.081ArgAsn: 3.081 ± 0.396
1.51ArgPro: 1.51 ± 0.261
1.571ArgGln: 1.571 ± 0.335
2.356ArgArg: 2.356 ± 0.33
2.356ArgSer: 2.356 ± 0.436
2.356ArgThr: 2.356 ± 0.389
3.383ArgVal: 3.383 ± 0.399
0.725ArgTrp: 0.725 ± 0.213
2.658ArgTyr: 2.658 ± 0.416
0.0ArgXaa: 0.0 ± 0.0
Ser
4.168SerAla: 4.168 ± 0.703
0.423SerCys: 0.423 ± 0.154
4.652SerAsp: 4.652 ± 0.473
4.289SerGlu: 4.289 ± 0.553
2.9SerPhe: 2.9 ± 0.405
4.41SerGly: 4.41 ± 0.462
1.148SerHis: 1.148 ± 0.247
3.987SerIle: 3.987 ± 0.372
4.108SerLys: 4.108 ± 0.461
5.256SerLeu: 5.256 ± 0.533
1.329SerMet: 1.329 ± 0.255
4.048SerAsn: 4.048 ± 0.652
1.812SerPro: 1.812 ± 0.305
2.054SerGln: 2.054 ± 0.435
2.719SerArg: 2.719 ± 0.398
3.564SerSer: 3.564 ± 0.452
3.383SerThr: 3.383 ± 0.434
4.893SerVal: 4.893 ± 0.461
0.906SerTrp: 0.906 ± 0.204
1.51SerTyr: 1.51 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
2.9ThrAla: 2.9 ± 0.484
0.967ThrCys: 0.967 ± 0.218
2.9ThrAsp: 2.9 ± 0.341
3.564ThrGlu: 3.564 ± 0.482
2.296ThrPhe: 2.296 ± 0.331
6.283ThrGly: 6.283 ± 0.595
1.208ThrHis: 1.208 ± 0.246
5.497ThrIle: 5.497 ± 0.684
3.262ThrLys: 3.262 ± 0.489
5.316ThrLeu: 5.316 ± 0.545
1.51ThrMet: 1.51 ± 0.305
2.296ThrAsn: 2.296 ± 0.347
2.719ThrPro: 2.719 ± 0.41
1.994ThrGln: 1.994 ± 0.326
2.416ThrArg: 2.416 ± 0.34
3.081ThrSer: 3.081 ± 0.442
2.416ThrThr: 2.416 ± 0.389
4.773ThrVal: 4.773 ± 0.655
0.785ThrTrp: 0.785 ± 0.235
1.752ThrTyr: 1.752 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
4.108ValAla: 4.108 ± 0.582
0.362ValCys: 0.362 ± 0.142
3.866ValAsp: 3.866 ± 0.374
5.377ValGlu: 5.377 ± 0.585
2.598ValPhe: 2.598 ± 0.371
4.652ValGly: 4.652 ± 0.439
0.785ValHis: 0.785 ± 0.258
5.8ValIle: 5.8 ± 0.44
5.014ValLys: 5.014 ± 0.58
4.229ValLeu: 4.229 ± 0.519
1.329ValMet: 1.329 ± 0.218
3.866ValAsn: 3.866 ± 0.473
1.873ValPro: 1.873 ± 0.338
0.906ValGln: 0.906 ± 0.209
3.081ValArg: 3.081 ± 0.397
5.618ValSer: 5.618 ± 0.707
4.712ValThr: 4.712 ± 0.666
2.9ValVal: 2.9 ± 0.476
0.967ValTrp: 0.967 ± 0.202
2.779ValTyr: 2.779 ± 0.475
0.0ValXaa: 0.0 ± 0.0
Trp
0.906TrpAla: 0.906 ± 0.223
0.242TrpCys: 0.242 ± 0.103
1.208TrpAsp: 1.208 ± 0.275
1.027TrpGlu: 1.027 ± 0.227
0.725TrpPhe: 0.725 ± 0.232
0.665TrpGly: 0.665 ± 0.195
0.302TrpHis: 0.302 ± 0.129
0.544TrpIle: 0.544 ± 0.175
0.846TrpLys: 0.846 ± 0.218
1.51TrpLeu: 1.51 ± 0.31
0.362TrpMet: 0.362 ± 0.148
0.725TrpAsn: 0.725 ± 0.21
0.0TrpPro: 0.0 ± 0.0
0.302TrpGln: 0.302 ± 0.116
0.846TrpArg: 0.846 ± 0.208
0.906TrpSer: 0.906 ± 0.222
0.725TrpThr: 0.725 ± 0.211
1.087TrpVal: 1.087 ± 0.214
0.362TrpTrp: 0.362 ± 0.153
0.906TrpTyr: 0.906 ± 0.262
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.175TyrAla: 2.175 ± 0.332
0.665TyrCys: 0.665 ± 0.193
2.719TyrAsp: 2.719 ± 0.484
3.504TyrGlu: 3.504 ± 0.486
1.812TyrPhe: 1.812 ± 0.324
2.9TyrGly: 2.9 ± 0.436
1.329TyrHis: 1.329 ± 0.258
2.175TyrIle: 2.175 ± 0.309
2.779TyrLys: 2.779 ± 0.44
2.839TyrLeu: 2.839 ± 0.474
0.967TyrMet: 0.967 ± 0.261
1.994TyrAsn: 1.994 ± 0.39
1.389TyrPro: 1.389 ± 0.362
1.51TyrGln: 1.51 ± 0.327
2.477TyrArg: 2.477 ± 0.355
2.537TyrSer: 2.537 ± 0.363
2.296TyrThr: 2.296 ± 0.292
2.114TyrVal: 2.114 ± 0.384
0.423TyrTrp: 0.423 ± 0.141
1.51TyrTyr: 1.51 ± 0.366
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16554 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski