Amino acid dipepetide frequency for Streptomyces phage Popy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.661AlaAla: 10.661 ± 1.55
0.227AlaCys: 0.227 ± 0.114
4.48AlaAsp: 4.48 ± 0.601
7.032AlaGlu: 7.032 ± 0.614
2.722AlaPhe: 2.722 ± 0.333
6.918AlaGly: 6.918 ± 1.331
1.645AlaHis: 1.645 ± 0.34
5.217AlaIle: 5.217 ± 0.557
6.918AlaLys: 6.918 ± 0.825
8.279AlaLeu: 8.279 ± 1.493
3.799AlaMet: 3.799 ± 0.469
2.835AlaAsn: 2.835 ± 0.337
2.665AlaPro: 2.665 ± 0.343
3.516AlaGln: 3.516 ± 0.482
4.31AlaArg: 4.31 ± 0.503
4.14AlaSer: 4.14 ± 0.508
5.898AlaThr: 5.898 ± 0.604
5.274AlaVal: 5.274 ± 0.478
1.021AlaTrp: 1.021 ± 0.244
3.346AlaTyr: 3.346 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.454CysAla: 0.454 ± 0.152
0.113CysCys: 0.113 ± 0.075
0.34CysAsp: 0.34 ± 0.136
0.284CysGlu: 0.284 ± 0.132
0.227CysPhe: 0.227 ± 0.12
0.454CysGly: 0.454 ± 0.243
0.057CysHis: 0.057 ± 0.061
0.227CysIle: 0.227 ± 0.135
0.17CysLys: 0.17 ± 0.122
0.794CysLeu: 0.794 ± 0.245
0.17CysMet: 0.17 ± 0.085
0.284CysAsn: 0.284 ± 0.134
0.397CysPro: 0.397 ± 0.186
0.17CysGln: 0.17 ± 0.088
0.17CysArg: 0.17 ± 0.119
0.34CysSer: 0.34 ± 0.136
0.227CysThr: 0.227 ± 0.09
0.17CysVal: 0.17 ± 0.111
0.17CysTrp: 0.17 ± 0.075
0.17CysTyr: 0.17 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
5.387AspAla: 5.387 ± 0.595
0.34AspCys: 0.34 ± 0.135
3.743AspAsp: 3.743 ± 0.619
6.408AspGlu: 6.408 ± 0.765
2.722AspPhe: 2.722 ± 0.474
5.274AspGly: 5.274 ± 0.635
0.907AspHis: 0.907 ± 0.251
3.686AspIle: 3.686 ± 0.483
3.062AspLys: 3.062 ± 0.431
4.14AspLeu: 4.14 ± 0.465
1.474AspMet: 1.474 ± 0.296
2.155AspAsn: 2.155 ± 0.394
4.026AspPro: 4.026 ± 0.684
1.985AspGln: 1.985 ± 0.397
3.516AspArg: 3.516 ± 0.666
3.459AspSer: 3.459 ± 0.432
3.062AspThr: 3.062 ± 0.379
3.119AspVal: 3.119 ± 0.418
1.418AspTrp: 1.418 ± 0.353
1.701AspTyr: 1.701 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
5.841GluAla: 5.841 ± 0.588
0.17GluCys: 0.17 ± 0.104
5.16GluAsp: 5.16 ± 0.478
6.125GluGlu: 6.125 ± 0.743
3.176GluPhe: 3.176 ± 0.476
5.898GluGly: 5.898 ± 0.502
1.191GluHis: 1.191 ± 0.291
4.083GluIle: 4.083 ± 0.463
4.99GluLys: 4.99 ± 0.459
6.635GluLeu: 6.635 ± 0.617
2.609GluMet: 2.609 ± 0.39
3.062GluAsn: 3.062 ± 0.359
2.552GluPro: 2.552 ± 0.403
2.382GluGln: 2.382 ± 0.284
4.48GluArg: 4.48 ± 0.552
3.856GluSer: 3.856 ± 0.355
3.856GluThr: 3.856 ± 0.446
5.104GluVal: 5.104 ± 0.623
1.077GluTrp: 1.077 ± 0.223
1.985GluTyr: 1.985 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
3.289PheAla: 3.289 ± 0.418
0.34PheCys: 0.34 ± 0.144
3.119PheAsp: 3.119 ± 0.443
2.949PheGlu: 2.949 ± 0.433
1.645PhePhe: 1.645 ± 0.316
2.552PheGly: 2.552 ± 0.404
0.397PheHis: 0.397 ± 0.136
1.701PheIle: 1.701 ± 0.397
1.871PheLys: 1.871 ± 0.351
1.985PheLeu: 1.985 ± 0.371
1.021PheMet: 1.021 ± 0.216
1.645PheAsn: 1.645 ± 0.225
1.191PhePro: 1.191 ± 0.26
1.645PheGln: 1.645 ± 0.262
2.382PheArg: 2.382 ± 0.389
2.098PheSer: 2.098 ± 0.456
2.552PheThr: 2.552 ± 0.483
2.212PheVal: 2.212 ± 0.361
0.567PheTrp: 0.567 ± 0.178
0.907PheTyr: 0.907 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
6.465GlyAla: 6.465 ± 0.908
0.454GlyCys: 0.454 ± 0.215
4.707GlyAsp: 4.707 ± 0.44
4.65GlyGlu: 4.65 ± 0.561
3.516GlyPhe: 3.516 ± 0.59
4.82GlyGly: 4.82 ± 0.643
1.077GlyHis: 1.077 ± 0.257
4.367GlyIle: 4.367 ± 0.897
4.48GlyLys: 4.48 ± 0.519
5.728GlyLeu: 5.728 ± 1.61
2.325GlyMet: 2.325 ± 0.308
2.892GlyAsn: 2.892 ± 0.452
2.325GlyPro: 2.325 ± 0.465
1.474GlyGln: 1.474 ± 0.314
3.062GlyArg: 3.062 ± 0.309
4.593GlySer: 4.593 ± 0.714
6.011GlyThr: 6.011 ± 0.756
6.465GlyVal: 6.465 ± 0.953
1.134GlyTrp: 1.134 ± 0.208
3.516GlyTyr: 3.516 ± 0.699
0.0GlyXaa: 0.0 ± 0.0
His
1.134HisAla: 1.134 ± 0.303
0.284HisCys: 0.284 ± 0.132
1.191HisAsp: 1.191 ± 0.248
1.134HisGlu: 1.134 ± 0.257
0.454HisPhe: 0.454 ± 0.188
1.815HisGly: 1.815 ± 0.356
0.624HisHis: 0.624 ± 0.164
1.134HisIle: 1.134 ± 0.285
1.191HisLys: 1.191 ± 0.273
1.077HisLeu: 1.077 ± 0.275
0.397HisMet: 0.397 ± 0.144
0.567HisAsn: 0.567 ± 0.19
1.021HisPro: 1.021 ± 0.232
0.794HisGln: 0.794 ± 0.205
0.794HisArg: 0.794 ± 0.249
1.304HisSer: 1.304 ± 0.254
0.624HisThr: 0.624 ± 0.197
1.645HisVal: 1.645 ± 0.316
0.397HisTrp: 0.397 ± 0.17
1.418HisTyr: 1.418 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
4.99IleAla: 4.99 ± 0.566
0.397IleCys: 0.397 ± 0.184
4.48IleAsp: 4.48 ± 0.481
4.31IleGlu: 4.31 ± 0.47
2.098IlePhe: 2.098 ± 0.365
3.289IleGly: 3.289 ± 0.783
1.418IleHis: 1.418 ± 0.333
2.098IleIle: 2.098 ± 0.517
3.459IleLys: 3.459 ± 0.574
4.253IleLeu: 4.253 ± 0.384
0.907IleMet: 0.907 ± 0.184
2.098IleAsn: 2.098 ± 0.306
2.438IlePro: 2.438 ± 0.31
1.588IleGln: 1.588 ± 0.259
2.892IleArg: 2.892 ± 0.372
3.459IleSer: 3.459 ± 0.466
3.913IleThr: 3.913 ± 0.507
3.629IleVal: 3.629 ± 0.381
0.227IleTrp: 0.227 ± 0.157
1.474IleTyr: 1.474 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
6.918LysAla: 6.918 ± 0.827
0.113LysCys: 0.113 ± 0.089
3.516LysAsp: 3.516 ± 0.59
4.026LysGlu: 4.026 ± 0.668
1.815LysPhe: 1.815 ± 0.275
4.31LysGly: 4.31 ± 0.775
1.304LysHis: 1.304 ± 0.286
3.346LysIle: 3.346 ± 0.44
6.748LysLys: 6.748 ± 1.006
6.521LysLeu: 6.521 ± 0.7
2.042LysMet: 2.042 ± 0.551
3.006LysAsn: 3.006 ± 0.52
2.212LysPro: 2.212 ± 0.452
2.382LysGln: 2.382 ± 0.329
3.403LysArg: 3.403 ± 0.549
4.65LysSer: 4.65 ± 0.566
4.31LysThr: 4.31 ± 0.573
4.367LysVal: 4.367 ± 0.589
1.191LysTrp: 1.191 ± 0.257
2.212LysTyr: 2.212 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
7.599LeuAla: 7.599 ± 1.286
0.397LeuCys: 0.397 ± 0.131
4.877LeuAsp: 4.877 ± 0.606
6.465LeuGlu: 6.465 ± 0.591
2.665LeuPhe: 2.665 ± 0.499
5.954LeuGly: 5.954 ± 1.159
1.871LeuHis: 1.871 ± 0.288
3.913LeuIle: 3.913 ± 0.617
6.011LeuLys: 6.011 ± 0.686
6.011LeuLeu: 6.011 ± 0.79
2.155LeuMet: 2.155 ± 0.333
2.835LeuAsn: 2.835 ± 0.311
2.212LeuPro: 2.212 ± 0.283
2.835LeuGln: 2.835 ± 0.387
4.48LeuArg: 4.48 ± 0.526
4.31LeuSer: 4.31 ± 0.457
6.011LeuThr: 6.011 ± 0.574
5.841LeuVal: 5.841 ± 0.735
1.077LeuTrp: 1.077 ± 0.205
2.495LeuTyr: 2.495 ± 0.297
0.0LeuXaa: 0.0 ± 0.0
Met
2.495MetAla: 2.495 ± 0.363
0.113MetCys: 0.113 ± 0.081
1.871MetAsp: 1.871 ± 0.297
1.588MetGlu: 1.588 ± 0.274
1.248MetPhe: 1.248 ± 0.233
1.418MetGly: 1.418 ± 0.239
0.17MetHis: 0.17 ± 0.079
1.418MetIle: 1.418 ± 0.371
1.758MetLys: 1.758 ± 0.339
2.609MetLeu: 2.609 ± 0.375
0.454MetMet: 0.454 ± 0.161
1.815MetAsn: 1.815 ± 0.313
1.304MetPro: 1.304 ± 0.291
0.737MetGln: 0.737 ± 0.212
1.531MetArg: 1.531 ± 0.268
2.212MetSer: 2.212 ± 0.382
2.155MetThr: 2.155 ± 0.415
1.077MetVal: 1.077 ± 0.235
0.34MetTrp: 0.34 ± 0.123
0.794MetTyr: 0.794 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
3.573AsnAla: 3.573 ± 0.299
0.454AsnCys: 0.454 ± 0.197
2.325AsnAsp: 2.325 ± 0.376
3.006AsnGlu: 3.006 ± 0.395
0.964AsnPhe: 0.964 ± 0.366
3.119AsnGly: 3.119 ± 0.42
1.077AsnHis: 1.077 ± 0.266
2.098AsnIle: 2.098 ± 0.301
2.438AsnLys: 2.438 ± 0.389
2.835AsnLeu: 2.835 ± 0.432
0.907AsnMet: 0.907 ± 0.223
1.758AsnAsn: 1.758 ± 0.386
2.438AsnPro: 2.438 ± 0.394
1.304AsnGln: 1.304 ± 0.297
2.552AsnArg: 2.552 ± 0.435
2.552AsnSer: 2.552 ± 0.384
2.609AsnThr: 2.609 ± 0.377
2.722AsnVal: 2.722 ± 0.344
0.397AsnTrp: 0.397 ± 0.14
1.474AsnTyr: 1.474 ± 0.256
0.0AsnXaa: 0.0 ± 0.0
Pro
2.722ProAla: 2.722 ± 0.397
0.284ProCys: 0.284 ± 0.111
3.289ProAsp: 3.289 ± 0.617
3.516ProGlu: 3.516 ± 0.587
1.361ProPhe: 1.361 ± 0.259
3.232ProGly: 3.232 ± 0.457
0.681ProHis: 0.681 ± 0.23
1.418ProIle: 1.418 ± 0.282
3.119ProLys: 3.119 ± 0.382
2.325ProLeu: 2.325 ± 0.396
0.964ProMet: 0.964 ± 0.279
1.531ProAsn: 1.531 ± 0.316
2.325ProPro: 2.325 ± 0.452
1.021ProGln: 1.021 ± 0.249
2.268ProArg: 2.268 ± 0.483
2.552ProSer: 2.552 ± 0.431
3.176ProThr: 3.176 ± 0.566
3.573ProVal: 3.573 ± 0.452
0.397ProTrp: 0.397 ± 0.143
1.191ProTyr: 1.191 ± 0.27
0.0ProXaa: 0.0 ± 0.0
Gln
3.062GlnAla: 3.062 ± 0.433
0.17GlnCys: 0.17 ± 0.093
1.645GlnAsp: 1.645 ± 0.233
2.098GlnGlu: 2.098 ± 0.419
0.907GlnPhe: 0.907 ± 0.22
2.268GlnGly: 2.268 ± 0.423
0.851GlnHis: 0.851 ± 0.268
2.268GlnIle: 2.268 ± 0.385
2.212GlnLys: 2.212 ± 0.294
3.289GlnLeu: 3.289 ± 0.485
0.681GlnMet: 0.681 ± 0.213
1.134GlnAsn: 1.134 ± 0.283
1.077GlnPro: 1.077 ± 0.263
1.418GlnGln: 1.418 ± 0.329
1.645GlnArg: 1.645 ± 0.268
2.212GlnSer: 2.212 ± 0.352
1.758GlnThr: 1.758 ± 0.333
2.495GlnVal: 2.495 ± 0.477
0.397GlnTrp: 0.397 ± 0.139
1.304GlnTyr: 1.304 ± 0.228
0.0GlnXaa: 0.0 ± 0.0
Arg
3.799ArgAla: 3.799 ± 0.551
0.51ArgCys: 0.51 ± 0.178
2.949ArgAsp: 2.949 ± 0.497
4.083ArgGlu: 4.083 ± 0.533
1.021ArgPhe: 1.021 ± 0.214
2.835ArgGly: 2.835 ± 0.416
1.134ArgHis: 1.134 ± 0.304
2.949ArgIle: 2.949 ± 0.446
4.537ArgLys: 4.537 ± 0.49
4.877ArgLeu: 4.877 ± 0.688
1.645ArgMet: 1.645 ± 0.276
2.552ArgAsn: 2.552 ± 0.457
2.268ArgPro: 2.268 ± 0.455
2.155ArgGln: 2.155 ± 0.467
3.459ArgArg: 3.459 ± 0.621
3.516ArgSer: 3.516 ± 0.491
3.006ArgThr: 3.006 ± 0.341
2.609ArgVal: 2.609 ± 0.399
0.624ArgTrp: 0.624 ± 0.21
1.474ArgTyr: 1.474 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
5.784SerAla: 5.784 ± 0.826
0.284SerCys: 0.284 ± 0.155
3.573SerAsp: 3.573 ± 0.485
4.196SerGlu: 4.196 ± 0.449
2.722SerPhe: 2.722 ± 0.413
5.331SerGly: 5.331 ± 0.531
1.077SerHis: 1.077 ± 0.253
2.722SerIle: 2.722 ± 0.375
3.913SerLys: 3.913 ± 0.632
4.82SerLeu: 4.82 ± 0.502
1.588SerMet: 1.588 ± 0.262
2.552SerAsn: 2.552 ± 0.395
2.609SerPro: 2.609 ± 0.351
1.928SerGln: 1.928 ± 0.283
2.495SerArg: 2.495 ± 0.408
4.65SerSer: 4.65 ± 0.815
3.856SerThr: 3.856 ± 0.519
4.31SerVal: 4.31 ± 0.456
1.248SerTrp: 1.248 ± 0.279
1.645SerTyr: 1.645 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
5.841ThrAla: 5.841 ± 0.703
0.227ThrCys: 0.227 ± 0.119
3.913ThrAsp: 3.913 ± 0.518
3.856ThrGlu: 3.856 ± 0.519
2.722ThrPhe: 2.722 ± 0.345
5.841ThrGly: 5.841 ± 0.571
1.191ThrHis: 1.191 ± 0.252
4.48ThrIle: 4.48 ± 0.571
3.856ThrLys: 3.856 ± 0.375
4.99ThrLeu: 4.99 ± 0.503
1.304ThrMet: 1.304 ± 0.276
1.588ThrAsn: 1.588 ± 0.217
3.573ThrPro: 3.573 ± 0.518
1.588ThrGln: 1.588 ± 0.316
2.609ThrArg: 2.609 ± 0.339
3.97ThrSer: 3.97 ± 0.653
5.274ThrThr: 5.274 ± 0.918
5.274ThrVal: 5.274 ± 0.547
1.304ThrTrp: 1.304 ± 0.314
2.098ThrTyr: 2.098 ± 0.38
0.0ThrXaa: 0.0 ± 0.0
Val
6.692ValAla: 6.692 ± 0.631
0.227ValCys: 0.227 ± 0.092
3.459ValAsp: 3.459 ± 0.419
5.16ValGlu: 5.16 ± 0.538
2.098ValPhe: 2.098 ± 0.45
4.764ValGly: 4.764 ± 0.578
1.077ValHis: 1.077 ± 0.257
3.743ValIle: 3.743 ± 0.437
4.877ValLys: 4.877 ± 0.485
5.728ValLeu: 5.728 ± 0.622
1.531ValMet: 1.531 ± 0.232
3.006ValAsn: 3.006 ± 0.402
2.722ValPro: 2.722 ± 0.52
1.985ValGln: 1.985 ± 0.359
3.799ValArg: 3.799 ± 0.433
4.083ValSer: 4.083 ± 0.446
4.707ValThr: 4.707 ± 0.545
4.14ValVal: 4.14 ± 0.55
1.191ValTrp: 1.191 ± 0.289
2.835ValTyr: 2.835 ± 0.581
0.0ValXaa: 0.0 ± 0.0
Trp
1.191TrpAla: 1.191 ± 0.281
0.113TrpCys: 0.113 ± 0.084
1.134TrpAsp: 1.134 ± 0.218
1.248TrpGlu: 1.248 ± 0.258
0.397TrpPhe: 0.397 ± 0.174
1.588TrpGly: 1.588 ± 0.272
0.34TrpHis: 0.34 ± 0.123
0.51TrpIle: 0.51 ± 0.193
0.737TrpLys: 0.737 ± 0.274
0.907TrpLeu: 0.907 ± 0.217
0.624TrpMet: 0.624 ± 0.211
1.134TrpAsn: 1.134 ± 0.272
0.34TrpPro: 0.34 ± 0.124
0.284TrpGln: 0.284 ± 0.12
0.34TrpArg: 0.34 ± 0.172
0.907TrpSer: 0.907 ± 0.256
1.077TrpThr: 1.077 ± 0.272
1.248TrpVal: 1.248 ± 0.327
0.17TrpTrp: 0.17 ± 0.094
0.454TrpTyr: 0.454 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.006TyrAla: 3.006 ± 0.597
0.17TyrCys: 0.17 ± 0.096
1.815TyrAsp: 1.815 ± 0.304
2.155TyrGlu: 2.155 ± 0.356
1.588TyrPhe: 1.588 ± 0.343
2.382TyrGly: 2.382 ± 0.414
0.907TyrHis: 0.907 ± 0.24
2.042TyrIle: 2.042 ± 0.342
2.042TyrLys: 2.042 ± 0.406
2.212TyrLeu: 2.212 ± 0.324
0.454TyrMet: 0.454 ± 0.153
2.155TyrAsn: 2.155 ± 0.398
1.248TyrPro: 1.248 ± 0.246
1.701TyrGln: 1.701 ± 0.358
1.815TyrArg: 1.815 ± 0.314
2.438TyrSer: 2.438 ± 0.378
1.418TyrThr: 1.418 ± 0.338
2.552TyrVal: 2.552 ± 0.314
0.397TyrTrp: 0.397 ± 0.162
1.531TyrTyr: 1.531 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (17635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski