Amino acid dipepetide frequency for Microbacterium phage Anakin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.021AlaAla: 15.021 ± 1.957
0.389AlaCys: 0.389 ± 0.14
6.231AlaAsp: 6.231 ± 0.57
8.679AlaGlu: 8.679 ± 0.852
3.227AlaPhe: 3.227 ± 0.316
7.177AlaGly: 7.177 ± 0.733
1.836AlaHis: 1.836 ± 0.375
5.619AlaIle: 5.619 ± 0.87
6.565AlaLys: 6.565 ± 1.089
9.124AlaLeu: 9.124 ± 0.724
2.837AlaMet: 2.837 ± 0.364
4.061AlaAsn: 4.061 ± 0.471
3.394AlaPro: 3.394 ± 0.548
4.618AlaGln: 4.618 ± 0.603
5.73AlaArg: 5.73 ± 0.633
5.174AlaSer: 5.174 ± 0.728
5.452AlaThr: 5.452 ± 0.786
6.565AlaVal: 6.565 ± 0.708
1.725AlaTrp: 1.725 ± 0.366
2.114AlaTyr: 2.114 ± 0.288
0.0AlaXaa: 0.0 ± 0.0
Cys
0.723CysAla: 0.723 ± 0.201
0.223CysCys: 0.223 ± 0.188
0.723CysAsp: 0.723 ± 0.19
0.501CysGlu: 0.501 ± 0.176
0.278CysPhe: 0.278 ± 0.114
0.89CysGly: 0.89 ± 0.258
0.167CysHis: 0.167 ± 0.092
0.111CysIle: 0.111 ± 0.069
0.111CysLys: 0.111 ± 0.071
0.278CysLeu: 0.278 ± 0.129
0.056CysMet: 0.056 ± 0.051
0.223CysAsn: 0.223 ± 0.115
0.612CysPro: 0.612 ± 0.234
0.223CysGln: 0.223 ± 0.109
0.223CysArg: 0.223 ± 0.122
0.501CysSer: 0.501 ± 0.198
0.445CysThr: 0.445 ± 0.157
0.445CysVal: 0.445 ± 0.167
0.056CysTrp: 0.056 ± 0.053
0.501CysTyr: 0.501 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
6.175AspAla: 6.175 ± 0.595
0.556AspCys: 0.556 ± 0.171
4.951AspAsp: 4.951 ± 0.845
6.898AspGlu: 6.898 ± 1.282
2.003AspPhe: 2.003 ± 0.305
6.565AspGly: 6.565 ± 0.774
0.723AspHis: 0.723 ± 0.213
4.117AspIle: 4.117 ± 0.491
2.837AspLys: 2.837 ± 0.448
6.398AspLeu: 6.398 ± 0.702
1.725AspMet: 1.725 ± 0.415
1.78AspAsn: 1.78 ± 0.27
4.84AspPro: 4.84 ± 0.571
1.836AspGln: 1.836 ± 0.315
3.783AspArg: 3.783 ± 0.429
2.782AspSer: 2.782 ± 0.324
3.616AspThr: 3.616 ± 0.457
4.006AspVal: 4.006 ± 0.428
1.224AspTrp: 1.224 ± 0.267
1.335AspTyr: 1.335 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
7.844GluAla: 7.844 ± 0.668
0.556GluCys: 0.556 ± 0.172
6.676GluAsp: 6.676 ± 1.016
7.177GluGlu: 7.177 ± 1.147
3.839GluPhe: 3.839 ± 0.484
4.673GluGly: 4.673 ± 0.547
2.225GluHis: 2.225 ± 0.36
3.616GluIle: 3.616 ± 0.444
4.117GluLys: 4.117 ± 0.493
5.341GluLeu: 5.341 ± 0.517
2.281GluMet: 2.281 ± 0.344
2.281GluAsn: 2.281 ± 0.369
3.004GluPro: 3.004 ± 0.559
2.949GluGln: 2.949 ± 0.383
4.951GluArg: 4.951 ± 0.737
2.726GluSer: 2.726 ± 0.379
3.95GluThr: 3.95 ± 0.504
4.451GluVal: 4.451 ± 0.675
1.28GluTrp: 1.28 ± 0.296
3.06GluTyr: 3.06 ± 0.348
0.0GluXaa: 0.0 ± 0.0
Phe
2.337PheAla: 2.337 ± 0.332
0.334PheCys: 0.334 ± 0.138
2.337PheAsp: 2.337 ± 0.282
2.615PheGlu: 2.615 ± 0.435
0.89PhePhe: 0.89 ± 0.287
3.115PheGly: 3.115 ± 0.374
0.723PheHis: 0.723 ± 0.195
1.446PheIle: 1.446 ± 0.222
1.669PheLys: 1.669 ± 0.308
2.67PheLeu: 2.67 ± 0.38
0.946PheMet: 0.946 ± 0.264
1.001PheAsn: 1.001 ± 0.216
1.836PhePro: 1.836 ± 0.344
0.946PheGln: 0.946 ± 0.247
2.17PheArg: 2.17 ± 0.305
2.003PheSer: 2.003 ± 0.295
1.725PheThr: 1.725 ± 0.295
1.502PheVal: 1.502 ± 0.27
0.556PheTrp: 0.556 ± 0.204
0.668PheTyr: 0.668 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
6.62GlyAla: 6.62 ± 0.595
0.334GlyCys: 0.334 ± 0.128
4.84GlyAsp: 4.84 ± 0.383
5.508GlyGlu: 5.508 ± 0.5
2.726GlyPhe: 2.726 ± 0.412
5.786GlyGly: 5.786 ± 0.623
1.335GlyHis: 1.335 ± 0.233
4.562GlyIle: 4.562 ± 0.54
4.451GlyLys: 4.451 ± 0.441
5.063GlyLeu: 5.063 ± 0.646
2.17GlyMet: 2.17 ± 0.278
2.559GlyAsn: 2.559 ± 0.37
2.503GlyPro: 2.503 ± 0.407
3.227GlyGln: 3.227 ± 0.368
4.395GlyArg: 4.395 ± 0.515
5.285GlySer: 5.285 ± 0.598
5.675GlyThr: 5.675 ± 0.713
5.675GlyVal: 5.675 ± 0.62
1.892GlyTrp: 1.892 ± 0.288
3.06GlyTyr: 3.06 ± 0.548
0.0GlyXaa: 0.0 ± 0.0
His
1.892HisAla: 1.892 ± 0.268
0.278HisCys: 0.278 ± 0.114
1.113HisAsp: 1.113 ± 0.269
0.834HisGlu: 0.834 ± 0.228
0.668HisPhe: 0.668 ± 0.201
1.558HisGly: 1.558 ± 0.334
0.501HisHis: 0.501 ± 0.158
0.612HisIle: 0.612 ± 0.137
0.834HisLys: 0.834 ± 0.233
1.224HisLeu: 1.224 ± 0.252
0.445HisMet: 0.445 ± 0.146
0.834HisAsn: 0.834 ± 0.245
1.168HisPro: 1.168 ± 0.283
0.445HisGln: 0.445 ± 0.181
1.28HisArg: 1.28 ± 0.249
0.834HisSer: 0.834 ± 0.202
1.502HisThr: 1.502 ± 0.359
1.168HisVal: 1.168 ± 0.29
0.389HisTrp: 0.389 ± 0.159
0.779HisTyr: 0.779 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.953IleAla: 5.953 ± 0.603
0.501IleCys: 0.501 ± 0.172
3.839IleAsp: 3.839 ± 0.488
3.839IleGlu: 3.839 ± 0.362
1.168IlePhe: 1.168 ± 0.256
3.95IleGly: 3.95 ± 0.667
0.612IleHis: 0.612 ± 0.221
2.615IleIle: 2.615 ± 0.419
2.448IleLys: 2.448 ± 0.333
3.561IleLeu: 3.561 ± 0.547
0.946IleMet: 0.946 ± 0.266
1.836IleAsn: 1.836 ± 0.28
1.947IlePro: 1.947 ± 0.347
0.946IleGln: 0.946 ± 0.247
3.394IleArg: 3.394 ± 0.429
2.058IleSer: 2.058 ± 0.428
3.616IleThr: 3.616 ± 0.415
3.505IleVal: 3.505 ± 0.459
1.502IleTrp: 1.502 ± 0.439
0.946IleTyr: 0.946 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
6.175LysAla: 6.175 ± 0.824
0.223LysCys: 0.223 ± 0.092
3.282LysAsp: 3.282 ± 0.475
2.949LysGlu: 2.949 ± 0.419
1.001LysPhe: 1.001 ± 0.199
3.505LysGly: 3.505 ± 0.48
0.612LysHis: 0.612 ± 0.172
2.837LysIle: 2.837 ± 0.422
3.115LysLys: 3.115 ± 0.506
4.117LysLeu: 4.117 ± 0.524
1.892LysMet: 1.892 ± 0.307
1.725LysAsn: 1.725 ± 0.288
2.281LysPro: 2.281 ± 0.438
1.947LysGln: 1.947 ± 0.315
3.227LysArg: 3.227 ± 0.465
2.949LysSer: 2.949 ± 0.422
3.561LysThr: 3.561 ± 0.519
3.839LysVal: 3.839 ± 0.418
1.057LysTrp: 1.057 ± 0.22
1.446LysTyr: 1.446 ± 0.307
0.0LysXaa: 0.0 ± 0.0
Leu
7.288LeuAla: 7.288 ± 0.621
0.445LeuCys: 0.445 ± 0.156
4.896LeuAsp: 4.896 ± 0.509
4.951LeuGlu: 4.951 ± 0.408
2.17LeuPhe: 2.17 ± 0.35
5.396LeuGly: 5.396 ± 0.607
1.224LeuHis: 1.224 ± 0.251
3.616LeuIle: 3.616 ± 0.556
4.117LeuLys: 4.117 ± 0.624
5.174LeuLeu: 5.174 ± 0.601
1.947LeuMet: 1.947 ± 0.296
2.949LeuAsn: 2.949 ± 0.425
4.395LeuPro: 4.395 ± 0.594
2.559LeuGln: 2.559 ± 0.314
4.951LeuArg: 4.951 ± 0.459
3.894LeuSer: 3.894 ± 0.541
5.174LeuThr: 5.174 ± 0.581
4.84LeuVal: 4.84 ± 0.549
1.335LeuTrp: 1.335 ± 0.241
1.725LeuTyr: 1.725 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
3.338MetAla: 3.338 ± 0.359
0.056MetCys: 0.056 ± 0.05
1.391MetAsp: 1.391 ± 0.307
1.335MetGlu: 1.335 ± 0.252
1.001MetPhe: 1.001 ± 0.177
1.669MetGly: 1.669 ± 0.351
0.223MetHis: 0.223 ± 0.11
1.502MetIle: 1.502 ± 0.314
1.001MetLys: 1.001 ± 0.227
2.114MetLeu: 2.114 ± 0.335
0.723MetMet: 0.723 ± 0.255
1.28MetAsn: 1.28 ± 0.268
1.725MetPro: 1.725 ± 0.325
0.834MetGln: 0.834 ± 0.265
1.113MetArg: 1.113 ± 0.245
2.17MetSer: 2.17 ± 0.354
1.947MetThr: 1.947 ± 0.352
1.28MetVal: 1.28 ± 0.259
0.501MetTrp: 0.501 ± 0.174
0.389MetTyr: 0.389 ± 0.133
0.0MetXaa: 0.0 ± 0.0
Asn
4.284AsnAla: 4.284 ± 0.764
0.278AsnCys: 0.278 ± 0.118
2.225AsnAsp: 2.225 ± 0.278
2.67AsnGlu: 2.67 ± 0.344
0.834AsnPhe: 0.834 ± 0.248
2.392AsnGly: 2.392 ± 0.407
1.001AsnHis: 1.001 ± 0.264
1.391AsnIle: 1.391 ± 0.342
1.446AsnLys: 1.446 ± 0.292
2.281AsnLeu: 2.281 ± 0.48
0.834AsnMet: 0.834 ± 0.224
1.947AsnAsn: 1.947 ± 0.411
2.114AsnPro: 2.114 ± 0.532
1.446AsnGln: 1.446 ± 0.26
2.448AsnArg: 2.448 ± 0.394
1.613AsnSer: 1.613 ± 0.321
2.726AsnThr: 2.726 ± 0.491
2.114AsnVal: 2.114 ± 0.394
0.612AsnTrp: 0.612 ± 0.194
0.723AsnTyr: 0.723 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
5.007ProAla: 5.007 ± 0.738
0.056ProCys: 0.056 ± 0.057
3.616ProAsp: 3.616 ± 0.546
5.063ProGlu: 5.063 ± 0.728
1.446ProPhe: 1.446 ± 0.302
3.672ProGly: 3.672 ± 0.51
0.946ProHis: 0.946 ± 0.204
2.337ProIle: 2.337 ± 0.355
3.171ProLys: 3.171 ± 0.501
3.394ProLeu: 3.394 ± 0.311
1.168ProMet: 1.168 ± 0.247
1.335ProAsn: 1.335 ± 0.332
1.725ProPro: 1.725 ± 0.33
1.669ProGln: 1.669 ± 0.254
2.114ProArg: 2.114 ± 0.353
3.004ProSer: 3.004 ± 0.423
3.783ProThr: 3.783 ± 0.532
3.171ProVal: 3.171 ± 0.457
0.834ProTrp: 0.834 ± 0.213
1.669ProTyr: 1.669 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
3.338GlnAla: 3.338 ± 0.352
0.167GlnCys: 0.167 ± 0.099
1.78GlnAsp: 1.78 ± 0.351
2.726GlnGlu: 2.726 ± 0.474
1.28GlnPhe: 1.28 ± 0.322
2.503GlnGly: 2.503 ± 0.292
1.057GlnHis: 1.057 ± 0.234
2.058GlnIle: 2.058 ± 0.358
2.003GlnLys: 2.003 ± 0.273
2.67GlnLeu: 2.67 ± 0.337
1.224GlnMet: 1.224 ± 0.237
1.168GlnAsn: 1.168 ± 0.328
2.114GlnPro: 2.114 ± 0.438
1.168GlnGln: 1.168 ± 0.336
2.67GlnArg: 2.67 ± 0.365
2.058GlnSer: 2.058 ± 0.367
2.281GlnThr: 2.281 ± 0.345
1.836GlnVal: 1.836 ± 0.261
0.779GlnTrp: 0.779 ± 0.235
0.723GlnTyr: 0.723 ± 0.219
0.0GlnXaa: 0.0 ± 0.0
Arg
5.897ArgAla: 5.897 ± 0.596
0.612ArgCys: 0.612 ± 0.219
4.228ArgAsp: 4.228 ± 0.469
3.95ArgGlu: 3.95 ± 0.525
2.003ArgPhe: 2.003 ± 0.335
3.727ArgGly: 3.727 ± 0.467
1.057ArgHis: 1.057 ± 0.231
2.837ArgIle: 2.837 ± 0.36
3.004ArgLys: 3.004 ± 0.448
4.172ArgLeu: 4.172 ± 0.52
2.003ArgMet: 2.003 ± 0.382
1.558ArgAsn: 1.558 ± 0.276
2.949ArgPro: 2.949 ± 0.462
3.06ArgGln: 3.06 ± 0.516
5.341ArgArg: 5.341 ± 0.678
3.115ArgSer: 3.115 ± 0.416
4.339ArgThr: 4.339 ± 0.704
4.339ArgVal: 4.339 ± 0.536
1.391ArgTrp: 1.391 ± 0.325
2.392ArgTyr: 2.392 ± 0.42
0.0ArgXaa: 0.0 ± 0.0
Ser
6.453SerAla: 6.453 ± 0.632
0.501SerCys: 0.501 ± 0.23
3.115SerAsp: 3.115 ± 0.503
4.172SerGlu: 4.172 ± 0.478
1.947SerPhe: 1.947 ± 0.366
6.12SerGly: 6.12 ± 0.482
1.001SerHis: 1.001 ± 0.258
2.003SerIle: 2.003 ± 0.406
2.949SerLys: 2.949 ± 0.456
3.505SerLeu: 3.505 ± 0.377
1.28SerMet: 1.28 ± 0.309
1.613SerAsn: 1.613 ± 0.313
2.67SerPro: 2.67 ± 0.411
1.947SerGln: 1.947 ± 0.32
2.726SerArg: 2.726 ± 0.378
2.837SerSer: 2.837 ± 0.382
2.503SerThr: 2.503 ± 0.432
3.394SerVal: 3.394 ± 0.404
1.168SerTrp: 1.168 ± 0.257
1.669SerTyr: 1.669 ± 0.361
0.0SerXaa: 0.0 ± 0.0
Thr
5.897ThrAla: 5.897 ± 0.782
0.501ThrCys: 0.501 ± 0.221
4.506ThrAsp: 4.506 ± 0.439
4.618ThrGlu: 4.618 ± 0.505
1.725ThrPhe: 1.725 ± 0.313
5.452ThrGly: 5.452 ± 0.719
1.224ThrHis: 1.224 ± 0.362
3.06ThrIle: 3.06 ± 0.488
2.949ThrLys: 2.949 ± 0.471
3.616ThrLeu: 3.616 ± 0.413
1.001ThrMet: 1.001 ± 0.237
2.337ThrAsn: 2.337 ± 0.422
4.117ThrPro: 4.117 ± 0.823
2.003ThrGln: 2.003 ± 0.253
4.451ThrArg: 4.451 ± 0.526
3.115ThrSer: 3.115 ± 0.437
4.228ThrThr: 4.228 ± 0.659
5.118ThrVal: 5.118 ± 0.375
1.446ThrTrp: 1.446 ± 0.231
2.281ThrTyr: 2.281 ± 0.346
0.0ThrXaa: 0.0 ± 0.0
Val
7.01ValAla: 7.01 ± 0.616
0.612ValCys: 0.612 ± 0.196
4.117ValAsp: 4.117 ± 0.437
4.784ValGlu: 4.784 ± 0.504
1.335ValPhe: 1.335 ± 0.306
5.675ValGly: 5.675 ± 0.742
0.612ValHis: 0.612 ± 0.2
3.004ValIle: 3.004 ± 0.365
3.004ValLys: 3.004 ± 0.357
5.118ValLeu: 5.118 ± 0.603
1.057ValMet: 1.057 ± 0.256
2.67ValAsn: 2.67 ± 0.458
3.616ValPro: 3.616 ± 0.81
2.225ValGln: 2.225 ± 0.378
3.783ValArg: 3.783 ± 0.512
4.784ValSer: 4.784 ± 0.613
4.506ValThr: 4.506 ± 0.557
4.896ValVal: 4.896 ± 0.698
1.502ValTrp: 1.502 ± 0.308
2.225ValTyr: 2.225 ± 0.349
0.0ValXaa: 0.0 ± 0.0
Trp
1.725TrpAla: 1.725 ± 0.342
0.278TrpCys: 0.278 ± 0.111
1.335TrpAsp: 1.335 ± 0.23
2.225TrpGlu: 2.225 ± 0.341
1.113TrpPhe: 1.113 ± 0.28
1.28TrpGly: 1.28 ± 0.3
0.668TrpHis: 0.668 ± 0.181
0.834TrpIle: 0.834 ± 0.191
0.668TrpLys: 0.668 ± 0.185
1.725TrpLeu: 1.725 ± 0.277
0.278TrpMet: 0.278 ± 0.121
0.834TrpAsn: 0.834 ± 0.364
1.001TrpPro: 1.001 ± 0.282
0.556TrpGln: 0.556 ± 0.173
1.168TrpArg: 1.168 ± 0.335
1.001TrpSer: 1.001 ± 0.22
1.001TrpThr: 1.001 ± 0.284
1.391TrpVal: 1.391 ± 0.294
0.334TrpTrp: 0.334 ± 0.133
0.668TrpTyr: 0.668 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.559TyrAla: 2.559 ± 0.419
0.389TyrCys: 0.389 ± 0.153
2.949TyrAsp: 2.949 ± 0.456
1.502TyrGlu: 1.502 ± 0.307
0.834TyrPhe: 0.834 ± 0.202
2.559TyrGly: 2.559 ± 0.364
0.612TyrHis: 0.612 ± 0.176
1.001TyrIle: 1.001 ± 0.185
1.28TyrLys: 1.28 ± 0.273
1.502TyrLeu: 1.502 ± 0.273
0.723TyrMet: 0.723 ± 0.215
1.391TyrAsn: 1.391 ± 0.331
1.168TyrPro: 1.168 ± 0.269
1.001TyrGln: 1.001 ± 0.271
2.058TyrArg: 2.058 ± 0.369
1.669TyrSer: 1.669 ± 0.317
1.613TyrThr: 1.613 ± 0.255
3.004TyrVal: 3.004 ± 0.457
0.501TyrTrp: 0.501 ± 0.162
0.668TyrTyr: 0.668 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (17976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski