Shahe tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGH4|A0A1L3KGH4_9VIRU RNA-directed RNA polymerase OS=Shahe tombus-like virus 1 OX=1923455 PE=4 SV=1
MM1 pKa = 8.04RR2 pKa = 11.84YY3 pKa = 9.28LAGFGTGVRR12 pKa = 11.84YY13 pKa = 9.22GVHH16 pKa = 6.16CFNYY20 pKa = 9.13TNLARR25 pKa = 11.84GIVEE29 pKa = 3.66RR30 pKa = 11.84VFYY33 pKa = 10.51VRR35 pKa = 11.84RR36 pKa = 11.84GEE38 pKa = 4.3GLAPAPQPSVGVFSRR53 pKa = 11.84LNAVRR58 pKa = 11.84QRR60 pKa = 11.84LLRR63 pKa = 11.84AVRR66 pKa = 11.84PTPVVARR73 pKa = 11.84DD74 pKa = 4.12EE75 pKa = 4.45YY76 pKa = 10.37PGLYY80 pKa = 9.16TGRR83 pKa = 11.84KK84 pKa = 6.95RR85 pKa = 11.84GVYY88 pKa = 9.29EE89 pKa = 4.09RR90 pKa = 11.84ALEE93 pKa = 4.1SLKK96 pKa = 10.48VRR98 pKa = 11.84AINFRR103 pKa = 11.84DD104 pKa = 2.98AWVNTFVKK112 pKa = 10.53AEE114 pKa = 4.02KK115 pKa = 10.82VNLDD119 pKa = 3.82SKK121 pKa = 11.25GDD123 pKa = 3.66PAPRR127 pKa = 11.84VIQPRR132 pKa = 11.84SPRR135 pKa = 11.84YY136 pKa = 7.87NLEE139 pKa = 3.41VGRR142 pKa = 11.84YY143 pKa = 8.32LKK145 pKa = 10.84LFEE148 pKa = 5.39KK149 pKa = 9.88EE150 pKa = 3.56LCAGFEE156 pKa = 4.39RR157 pKa = 11.84VWGYY161 pKa = 9.91PVVLKK166 pKa = 10.78GKK168 pKa = 9.18NAQDD172 pKa = 3.14VGAAMADD179 pKa = 3.18HH180 pKa = 7.15WQQFAQPVAVGLDD193 pKa = 3.16ASRR196 pKa = 11.84FDD198 pKa = 3.43QHH200 pKa = 6.33VSKK203 pKa = 11.0DD204 pKa = 3.37ALRR207 pKa = 11.84WEE209 pKa = 4.13HH210 pKa = 6.33SVYY213 pKa = 10.99NSVFNSPEE221 pKa = 3.92LRR223 pKa = 11.84RR224 pKa = 11.84LLQWQLRR231 pKa = 11.84NHH233 pKa = 7.01GIARR237 pKa = 11.84VEE239 pKa = 4.02DD240 pKa = 4.69LIVEE244 pKa = 4.0YY245 pKa = 10.41DD246 pKa = 3.32IEE248 pKa = 4.54GCRR251 pKa = 11.84MSGDD255 pKa = 3.38INTGLGNCLIMSSIVIAYY273 pKa = 9.24CEE275 pKa = 3.5QAGIYY280 pKa = 9.75FRR282 pKa = 11.84LANNGDD288 pKa = 3.76DD289 pKa = 4.07CVLFVDD295 pKa = 5.11KK296 pKa = 11.27ADD298 pKa = 4.1LGALAGIDD306 pKa = 3.32QWFLDD311 pKa = 3.99FGFTLTRR318 pKa = 11.84EE319 pKa = 4.02EE320 pKa = 3.98PVYY323 pKa = 10.98VLEE326 pKa = 4.87QVVFCQAQPVLTSTGWRR343 pKa = 11.84MVRR346 pKa = 11.84DD347 pKa = 3.68PRR349 pKa = 11.84TAMSKK354 pKa = 10.71DD355 pKa = 3.63CVSLLGWDD363 pKa = 3.53NAQSFKK369 pKa = 10.83LWASAIGACGLSLTRR384 pKa = 11.84GVPVWEE390 pKa = 3.92AWYY393 pKa = 10.75SRR395 pKa = 11.84LVHH398 pKa = 6.59LGAGAVSQGVADD410 pKa = 4.42HH411 pKa = 6.43VWDD414 pKa = 3.87SGLGYY419 pKa = 9.67MSRR422 pKa = 11.84GVLGGEE428 pKa = 3.78VDD430 pKa = 3.31AEE432 pKa = 4.2ARR434 pKa = 11.84VSFYY438 pKa = 10.7RR439 pKa = 11.84AFGILPDD446 pKa = 3.75LQEE449 pKa = 4.3ALEE452 pKa = 4.48AEE454 pKa = 5.06YY455 pKa = 10.71ACPMPIGSPTPMMHH469 pKa = 6.79PSIVAIDD476 pKa = 3.82TTCNPLATWLAAKK489 pKa = 10.11

Molecular weight: 54.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGH4|A0A1L3KGH4_9VIRU RNA-directed RNA polymerase OS=Shahe tombus-like virus 1 OX=1923455 PE=4 SV=1
MM1 pKa = 7.65ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 10.0VMPSPVQGGVRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84NKK21 pKa = 9.79QPNVTQTSGTSSVIKK36 pKa = 10.28YY37 pKa = 9.36SALGSTVTSDD47 pKa = 3.5VNGEE51 pKa = 3.59AWYY54 pKa = 9.86HH55 pKa = 5.53RR56 pKa = 11.84MYY58 pKa = 10.77IPGSFGGLTQTVGTAIVGYY77 pKa = 10.04YY78 pKa = 8.85STAKK82 pKa = 9.82FQAGTTLRR90 pKa = 11.84WEE92 pKa = 4.26PSVSFSTSGRR102 pKa = 11.84VYY104 pKa = 11.03VGFTDD109 pKa = 3.53NPEE112 pKa = 4.1MIEE115 pKa = 3.91AMRR118 pKa = 11.84VLTGGNAVNAVKK130 pKa = 10.77GLGDD134 pKa = 3.86VISFPVWQEE143 pKa = 3.06TDD145 pKa = 3.01IPFSPRR151 pKa = 11.84MRR153 pKa = 11.84RR154 pKa = 11.84KK155 pKa = 9.4MFDD158 pKa = 3.15TNQAAITGVDD168 pKa = 3.53VLDD171 pKa = 4.3RR172 pKa = 11.84TCQTAMFLAIDD183 pKa = 4.33GMPASTRR190 pKa = 11.84AGSVWFHH197 pKa = 7.27DD198 pKa = 3.9NVAVEE203 pKa = 4.42GMSSIEE209 pKa = 4.03TT210 pKa = 3.67

Molecular weight: 22.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

699

210

489

349.5

38.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.156 ± 0.855

1.717 ± 0.69

5.007 ± 0.401

4.578 ± 0.692

4.149 ± 0.076

9.156 ± 0.469

1.431 ± 0.266

3.72 ± 0.315

2.861 ± 0.002

7.582 ± 2.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 1.057

3.577 ± 0.13

4.864 ± 0.057

3.72 ± 0.05

7.725 ± 0.324

6.438 ± 1.451

5.293 ± 2.618

10.157 ± 0.177

2.432 ± 0.293

3.577 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski