Amino acid dipepetide frequency for Quadrisphaera granulorum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	26.64AlaAla: 26.64 ± 0.259	1.04AlaCys: 1.04 ± 0.026	8.472AlaAsp: 8.472 ± 0.084	8.349AlaGlu: 8.349 ± 0.13	3.422AlaPhe: 3.422 ± 0.058	14.371AlaGly: 14.371 ± 0.122	2.84AlaHis: 2.84 ± 0.048	3.307AlaIle: 3.307 ± 0.062	2.001AlaLys: 2.001 ± 0.043	17.112AlaLeu: 17.112 ± 0.147	2.481AlaMet: 2.481 ± 0.039	1.62AlaAsn: 1.62 ± 0.039	8.012AlaPro: 8.012 ± 0.093	4.544AlaGln: 4.544 ± 0.056	11.051AlaArg: 11.051 ± 0.119	8.432AlaSer: 8.432 ± 0.081	8.246AlaThr: 8.246 ± 0.081	15.481AlaVal: 15.481 ± 0.158	2.323AlaTrp: 2.323 ± 0.048	2.06AlaTyr: 2.06 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.915CysAla: 0.915 ± 0.031	0.069CysCys: 0.069 ± 0.007	0.393CysAsp: 0.393 ± 0.016	0.3CysGlu: 0.3 ± 0.016	0.189CysPhe: 0.189 ± 0.011	0.762CysGly: 0.762 ± 0.025	0.133CysHis: 0.133 ± 0.009	0.147CysIle: 0.147 ± 0.011	0.066CysLys: 0.066 ± 0.007	0.627CysLeu: 0.627 ± 0.023	0.086CysMet: 0.086 ± 0.008	0.086CysAsn: 0.086 ± 0.008	0.407CysPro: 0.407 ± 0.018	0.141CysGln: 0.141 ± 0.01	0.494CysArg: 0.494 ± 0.02	0.433CysSer: 0.433 ± 0.018	0.443CysThr: 0.443 ± 0.017	0.55CysVal: 0.55 ± 0.02	0.115CysTrp: 0.115 ± 0.008	0.12CysTyr: 0.12 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.826AspAla: 8.826 ± 0.085	0.282AspCys: 0.282 ± 0.015	5.302AspAsp: 5.302 ± 0.071	3.542AspGlu: 3.542 ± 0.057	1.144AspPhe: 1.144 ± 0.029	6.604AspGly: 6.604 ± 0.081	1.354AspHis: 1.354 ± 0.034	1.238AspIle: 1.238 ± 0.035	0.669AspLys: 0.669 ± 0.025	6.795AspLeu: 6.795 ± 0.07	0.574AspMet: 0.574 ± 0.027	0.583AspAsn: 0.583 ± 0.021	4.482AspPro: 4.482 ± 0.06	1.433AspGln: 1.433 ± 0.032	4.122AspArg: 4.122 ± 0.059	2.207AspSer: 2.207 ± 0.038	2.407AspThr: 2.407 ± 0.041	6.617AspVal: 6.617 ± 0.074	0.794AspTrp: 0.794 ± 0.026	0.878AspTyr: 0.878 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.481GluAla: 7.481 ± 0.126	0.267GluCys: 0.267 ± 0.013	2.519GluAsp: 2.519 ± 0.043	2.882GluGlu: 2.882 ± 0.054	0.974GluPhe: 0.974 ± 0.028	3.973GluGly: 3.973 ± 0.059	1.782GluHis: 1.782 ± 0.038	1.335GluIle: 1.335 ± 0.038	0.854GluLys: 0.854 ± 0.028	6.422GluLeu: 6.422 ± 0.086	0.671GluMet: 0.671 ± 0.023	0.664GluAsn: 0.664 ± 0.027	3.067GluPro: 3.067 ± 0.048	2.531GluGln: 2.531 ± 0.049	5.474GluArg: 5.474 ± 0.074	2.018GluSer: 2.018 ± 0.041	2.197GluThr: 2.197 ± 0.039	5.649GluVal: 5.649 ± 0.07	0.621GluTrp: 0.621 ± 0.02	0.675GluTyr: 0.675 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.431PheAla: 3.431 ± 0.058	0.194PheCys: 0.194 ± 0.012	1.639PheAsp: 1.639 ± 0.033	1.123PheGlu: 1.123 ± 0.028	0.678PhePhe: 0.678 ± 0.026	2.516PheGly: 2.516 ± 0.042	0.439PheHis: 0.439 ± 0.018	0.638PheIle: 0.638 ± 0.023	0.325PheLys: 0.325 ± 0.015	2.11PheLeu: 2.11 ± 0.046	0.304PheMet: 0.304 ± 0.014	0.394PheAsn: 0.394 ± 0.017	1.104PhePro: 1.104 ± 0.03	0.541PheGln: 0.541 ± 0.02	1.36PheArg: 1.36 ± 0.035	1.423PheSer: 1.423 ± 0.032	1.884PheThr: 1.884 ± 0.04	2.206PheVal: 2.206 ± 0.043	0.354PheTrp: 0.354 ± 0.016	0.439PheTyr: 0.439 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	12.817GlyAla: 12.817 ± 0.111	0.742GlyCys: 0.742 ± 0.023	5.087GlyAsp: 5.087 ± 0.063	4.716GlyGlu: 4.716 ± 0.061	2.529GlyPhe: 2.529 ± 0.046	8.913GlyGly: 8.913 ± 0.113	1.99GlyHis: 1.99 ± 0.038	2.809GlyIle: 2.809 ± 0.048	1.587GlyLys: 1.587 ± 0.04	9.8GlyLeu: 9.8 ± 0.101	1.675GlyMet: 1.675 ± 0.037	1.261GlyAsn: 1.261 ± 0.033	5.022GlyPro: 5.022 ± 0.073	2.817GlyGln: 2.817 ± 0.04	7.468GlyArg: 7.468 ± 0.068	5.836GlySer: 5.836 ± 0.072	5.81GlyThr: 5.81 ± 0.079	8.862GlyVal: 8.862 ± 0.089	1.837GlyTrp: 1.837 ± 0.045	1.891GlyTyr: 1.891 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	2.693HisAla: 2.693 ± 0.045	0.138HisCys: 0.138 ± 0.009	1.384HisAsp: 1.384 ± 0.033	1.162HisGlu: 1.162 ± 0.028	0.443HisPhe: 0.443 ± 0.016	2.107HisGly: 2.107 ± 0.043	0.674HisHis: 0.674 ± 0.021	0.379HisIle: 0.379 ± 0.015	0.175HisLys: 0.175 ± 0.01	2.792HisLeu: 2.792 ± 0.043	0.218HisMet: 0.218 ± 0.012	0.245HisAsn: 0.245 ± 0.013	1.665HisPro: 1.665 ± 0.038	0.6HisGln: 0.6 ± 0.021	2.007HisArg: 2.007 ± 0.043	0.896HisSer: 0.896 ± 0.026	1.025HisThr: 1.025 ± 0.029	1.997HisVal: 1.997 ± 0.041	0.294HisTrp: 0.294 ± 0.016	0.35HisTyr: 0.35 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.295IleAla: 4.295 ± 0.066	0.207IleCys: 0.207 ± 0.013	1.941IleAsp: 1.941 ± 0.036	1.363IleGlu: 1.363 ± 0.035	0.574IlePhe: 0.574 ± 0.022	2.967IleGly: 2.967 ± 0.049	0.405IleHis: 0.405 ± 0.017	0.722IleIle: 0.722 ± 0.025	0.435IleLys: 0.435 ± 0.018	1.527IleLeu: 1.527 ± 0.043	0.301IleMet: 0.301 ± 0.015	0.511IleAsn: 0.511 ± 0.022	1.272IlePro: 1.272 ± 0.035	0.505IleGln: 0.505 ± 0.021	1.356IleArg: 1.356 ± 0.031	1.615IleSer: 1.615 ± 0.036	2.028IleThr: 2.028 ± 0.043	2.126IleVal: 2.126 ± 0.047	0.27IleTrp: 0.27 ± 0.015	0.407IleTyr: 0.407 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.039LysAla: 2.039 ± 0.049	0.059LysCys: 0.059 ± 0.007	0.839LysAsp: 0.839 ± 0.029	0.631LysGlu: 0.631 ± 0.021	0.271LysPhe: 0.271 ± 0.016	1.191LysGly: 1.191 ± 0.032	0.33LysHis: 0.33 ± 0.014	0.532LysIle: 0.532 ± 0.022	0.391LysLys: 0.391 ± 0.02	1.32LysLeu: 1.32 ± 0.032	0.197LysMet: 0.197 ± 0.011	0.29LysAsn: 0.29 ± 0.016	0.904LysPro: 0.904 ± 0.027	0.439LysGln: 0.439 ± 0.019	1.027LysArg: 1.027 ± 0.027	0.721LysSer: 0.721 ± 0.025	0.935LysThr: 0.935 ± 0.032	1.321LysVal: 1.321 ± 0.035	0.138LysTrp: 0.138 ± 0.01	0.248LysTyr: 0.248 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	17.696LeuAla: 17.696 ± 0.14	0.638LeuCys: 0.638 ± 0.024	7.164LeuAsp: 7.164 ± 0.082	5.392LeuGlu: 5.392 ± 0.078	2.25LeuPhe: 2.25 ± 0.049	9.724LeuGly: 9.724 ± 0.098	2.198LeuHis: 2.198 ± 0.041	2.217LeuIle: 2.217 ± 0.043	1.272LeuLys: 1.272 ± 0.036	12.826LeuLeu: 12.826 ± 0.141	1.282LeuMet: 1.282 ± 0.032	1.281LeuAsn: 1.281 ± 0.033	6.685LeuPro: 6.685 ± 0.07	2.703LeuGln: 2.703 ± 0.043	9.119LeuArg: 9.119 ± 0.093	5.619LeuSer: 5.619 ± 0.073	6.299LeuThr: 6.299 ± 0.073	11.881LeuVal: 11.881 ± 0.116	1.242LeuTrp: 1.242 ± 0.033	1.38LeuTyr: 1.38 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.138MetAla: 2.138 ± 0.042	0.084MetCys: 0.084 ± 0.008	0.709MetAsp: 0.709 ± 0.024	0.519MetGlu: 0.519 ± 0.023	0.301MetPhe: 0.301 ± 0.015	1.12MetGly: 1.12 ± 0.028	0.32MetHis: 0.32 ± 0.014	0.477MetIle: 0.477 ± 0.019	0.26MetLys: 0.26 ± 0.012	1.426MetLeu: 1.426 ± 0.03	0.193MetMet: 0.193 ± 0.012	0.29MetAsn: 0.29 ± 0.013	0.964MetPro: 0.964 ± 0.026	0.384MetGln: 0.384 ± 0.019	1.148MetArg: 1.148 ± 0.029	1.378MetSer: 1.378 ± 0.029	1.474MetThr: 1.474 ± 0.031	1.18MetVal: 1.18 ± 0.027	0.141MetTrp: 0.141 ± 0.011	0.2MetTyr: 0.2 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	1.933AsnAla: 1.933 ± 0.043	0.107AsnCys: 0.107 ± 0.008	0.806AsnAsp: 0.806 ± 0.025	0.524AsnGlu: 0.524 ± 0.02	0.345AsnPhe: 0.345 ± 0.017	1.367AsnGly: 1.367 ± 0.037	0.299AsnHis: 0.299 ± 0.014	0.41AsnIle: 0.41 ± 0.02	0.201AsnLys: 0.201 ± 0.012	1.386AsnLeu: 1.386 ± 0.031	0.152AsnMet: 0.152 ± 0.012	0.292AsnAsn: 0.292 ± 0.018	1.122AsnPro: 1.122 ± 0.029	0.374AsnGln: 0.374 ± 0.016	0.874AsnArg: 0.874 ± 0.027	0.629AsnSer: 0.629 ± 0.022	0.81AsnThr: 0.81 ± 0.026	1.076AsnVal: 1.076 ± 0.033	0.188AsnTrp: 0.188 ± 0.012	0.263AsnTyr: 0.263 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	9.038ProAla: 9.038 ± 0.097	0.262ProCys: 0.262 ± 0.015	4.027ProAsp: 4.027 ± 0.064	3.658ProGlu: 3.658 ± 0.052	1.419ProPhe: 1.419 ± 0.028	6.211ProGly: 6.211 ± 0.071	1.258ProHis: 1.258 ± 0.03	1.124ProIle: 1.124 ± 0.034	0.755ProLys: 0.755 ± 0.022	5.871ProLeu: 5.871 ± 0.075	0.883ProMet: 0.883 ± 0.025	0.647ProAsn: 0.647 ± 0.023	3.41ProPro: 3.41 ± 0.065	1.778ProGln: 1.778 ± 0.036	4.099ProArg: 4.099 ± 0.058	3.917ProSer: 3.917 ± 0.061	3.738ProThr: 3.738 ± 0.064	5.758ProVal: 5.758 ± 0.065	0.97ProTrp: 0.97 ± 0.028	0.871ProTyr: 0.871 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.886GlnAla: 3.886 ± 0.061	0.15GlnCys: 0.15 ± 0.01	1.261GlnAsp: 1.261 ± 0.032	1.246GlnGlu: 1.246 ± 0.03	0.565GlnPhe: 0.565 ± 0.023	2.153GlnGly: 2.153 ± 0.039	0.744GlnHis: 0.744 ± 0.024	0.806GlnIle: 0.806 ± 0.027	0.386GlnLys: 0.386 ± 0.015	3.852GlnLeu: 3.852 ± 0.051	0.463GlnMet: 0.463 ± 0.018	0.375GlnAsn: 0.375 ± 0.016	1.824GlnPro: 1.824 ± 0.041	1.376GlnGln: 1.376 ± 0.039	3.102GlnArg: 3.102 ± 0.057	1.144GlnSer: 1.144 ± 0.027	1.462GlnThr: 1.462 ± 0.037	3.139GlnVal: 3.139 ± 0.046	0.432GlnTrp: 0.432 ± 0.019	0.476GlnTyr: 0.476 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	11.26ArgAla: 11.26 ± 0.122	0.533ArgCys: 0.533 ± 0.023	4.215ArgAsp: 4.215 ± 0.063	4.52ArgGlu: 4.52 ± 0.078	1.924ArgPhe: 1.924 ± 0.042	6.26ArgGly: 6.26 ± 0.063	1.691ArgHis: 1.691 ± 0.038	2.274ArgIle: 2.274 ± 0.042	0.973ArgLys: 0.973 ± 0.027	8.491ArgLeu: 8.491 ± 0.087	1.52ArgMet: 1.52 ± 0.036	0.946ArgAsn: 0.946 ± 0.028	4.508ArgPro: 4.508 ± 0.055	2.226ArgGln: 2.226 ± 0.04	8.261ArgArg: 8.261 ± 0.102	4.807ArgSer: 4.807 ± 0.064	5.047ArgThr: 5.047 ± 0.067	6.685ArgVal: 6.685 ± 0.076	1.672ArgTrp: 1.672 ± 0.043	1.439ArgTyr: 1.439 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	8.629SerAla: 8.629 ± 0.088	0.335SerCys: 0.335 ± 0.013	2.832SerAsp: 2.832 ± 0.038	2.451SerGlu: 2.451 ± 0.041	1.439SerPhe: 1.439 ± 0.036	6.237SerGly: 6.237 ± 0.073	0.967SerHis: 0.967 ± 0.027	1.463SerIle: 1.463 ± 0.035	0.765SerLys: 0.765 ± 0.025	5.385SerLeu: 5.385 ± 0.074	1.079SerMet: 1.079 ± 0.027	0.788SerAsn: 0.788 ± 0.023	3.579SerPro: 3.579 ± 0.047	1.538SerGln: 1.538 ± 0.035	4.028SerArg: 4.028 ± 0.047	4.382SerSer: 4.382 ± 0.083	4.163SerThr: 4.163 ± 0.056	4.708SerVal: 4.708 ± 0.048	0.942SerTrp: 0.942 ± 0.03	0.941SerTyr: 0.941 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	9.759ThrAla: 9.759 ± 0.097	0.4ThrCys: 0.4 ± 0.015	3.396ThrAsp: 3.396 ± 0.05	2.634ThrGlu: 2.634 ± 0.043	1.503ThrPhe: 1.503 ± 0.034	6.375ThrGly: 6.375 ± 0.065	1.106ThrHis: 1.106 ± 0.028	1.507ThrIle: 1.507 ± 0.037	0.857ThrLys: 0.857 ± 0.03	5.047ThrLeu: 5.047 ± 0.065	0.784ThrMet: 0.784 ± 0.024	0.89ThrAsn: 0.89 ± 0.028	4.289ThrPro: 4.289 ± 0.073	1.444ThrGln: 1.444 ± 0.03	3.832ThrArg: 3.832 ± 0.053	4.291ThrSer: 4.291 ± 0.051	5.018ThrThr: 5.018 ± 0.08	5.527ThrVal: 5.527 ± 0.068	1.043ThrTrp: 1.043 ± 0.028	1.042ThrTyr: 1.042 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	14.232ValAla: 14.232 ± 0.118	0.701ValCys: 0.701 ± 0.025	6.017ValAsp: 6.017 ± 0.075	5.578ValGlu: 5.578 ± 0.069	2.23ValPhe: 2.23 ± 0.047	7.926ValGly: 7.926 ± 0.092	2.126ValHis: 2.126 ± 0.037	2.719ValIle: 2.719 ± 0.046	1.389ValLys: 1.389 ± 0.036	12.504ValLeu: 12.504 ± 0.107	1.42ValMet: 1.42 ± 0.032	1.431ValAsn: 1.431 ± 0.036	5.753ValPro: 5.753 ± 0.069	2.472ValGln: 2.472 ± 0.043	7.621ValArg: 7.621 ± 0.078	5.078ValSer: 5.078 ± 0.058	5.795ValThr: 5.795 ± 0.065	13.223ValVal: 13.223 ± 0.126	1.183ValTrp: 1.183 ± 0.027	1.284ValTyr: 1.284 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.89TrpAla: 1.89 ± 0.043	0.142TrpCys: 0.142 ± 0.011	0.808TrpAsp: 0.808 ± 0.027	0.628TrpGlu: 0.628 ± 0.018	0.441TrpPhe: 0.441 ± 0.017	1.114TrpGly: 1.114 ± 0.028	0.355TrpHis: 0.355 ± 0.015	0.422TrpIle: 0.422 ± 0.017	0.252TrpLys: 0.252 ± 0.014	1.781TrpLeu: 1.781 ± 0.047	0.27TrpMet: 0.27 ± 0.012	0.292TrpAsn: 0.292 ± 0.016	0.803TrpPro: 0.803 ± 0.03	0.562TrpGln: 0.562 ± 0.023	1.406TrpArg: 1.406 ± 0.034	1.017TrpSer: 1.017 ± 0.03	1.086TrpThr: 1.086 ± 0.03	1.299TrpVal: 1.299 ± 0.03	0.4TrpTrp: 0.4 ± 0.02	0.244TrpTyr: 0.244 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.208TyrAla: 2.208 ± 0.043	0.124TyrCys: 0.124 ± 0.009	1.039TyrAsp: 1.039 ± 0.028	0.839TyrGlu: 0.839 ± 0.028	0.461TyrPhe: 0.461 ± 0.02	1.58TyrGly: 1.58 ± 0.039	0.235TyrHis: 0.235 ± 0.012	0.301TyrIle: 0.301 ± 0.015	0.211TyrLys: 0.211 ± 0.011	1.796TyrLeu: 1.796 ± 0.035	0.152TyrMet: 0.152 ± 0.01	0.269TyrAsn: 0.269 ± 0.014	0.826TyrPro: 0.826 ± 0.025	0.435TyrGln: 0.435 ± 0.018	1.403TyrArg: 1.403 ± 0.032	0.808TyrSer: 0.808 ± 0.027	0.879TyrThr: 0.879 ± 0.029	1.394TyrVal: 1.394 ± 0.033	0.25TyrTrp: 0.25 ± 0.015	0.322TyrTyr: 0.322 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4581 proteins (1470649 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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