Cellulomonas shaoxiangyii
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3337 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1CME5|A0A4V1CME5_9CELL GNAT family N-acetyltransferase OS=Cellulomonas shaoxiangyii OX=2566013 GN=E5225_02975 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.29 RR3 pKa = 11.84 IQRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 GLTLAASVAGLGLVLTACSGGDD30 pKa = 3.44 SGDD33 pKa = 3.82 GGDD36 pKa = 4.22 GGTTGGAGEE45 pKa = 4.24 AAVDD49 pKa = 3.49 CSEE52 pKa = 3.95 FEE54 pKa = 4.15 EE55 pKa = 4.76 YY56 pKa = 11.18 GDD58 pKa = 4.79 LEE60 pKa = 4.56 GTTVTVYY67 pKa = 10.41 TSIVDD72 pKa = 3.77 PEE74 pKa = 4.38 AEE76 pKa = 4.14 QQRR79 pKa = 11.84 DD80 pKa = 3.32 SYY82 pKa = 12.02 APFEE86 pKa = 4.36 QCTGATIEE94 pKa = 4.29 YY95 pKa = 8.59 EE96 pKa = 4.15 GSRR99 pKa = 11.84 EE100 pKa = 3.93 FEE102 pKa = 3.84 AQLPVRR108 pKa = 11.84 LEE110 pKa = 4.11 AGNPPDD116 pKa = 3.65 IAYY119 pKa = 9.42 IPQPGFLASLVAEE132 pKa = 4.45 YY133 pKa = 10.42 PDD135 pKa = 3.66 VVQAAPEE142 pKa = 4.02 AVVDD146 pKa = 4.03 NANEE150 pKa = 4.26 YY151 pKa = 10.9 YY152 pKa = 10.32 SEE154 pKa = 3.96 EE155 pKa = 3.94 WVNYY159 pKa = 7.04 GTVDD163 pKa = 3.13 GTLYY167 pKa = 9.35 ATPLGANVKK176 pKa = 10.15 SFVWYY181 pKa = 10.36 SPALFEE187 pKa = 4.65 EE188 pKa = 4.38 NGYY191 pKa = 9.86 EE192 pKa = 5.91 IPATWQDD199 pKa = 4.2 LLDD202 pKa = 4.06 LTDD205 pKa = 5.55 QIVEE209 pKa = 4.61 DD210 pKa = 4.48 NPDD213 pKa = 3.55 AKK215 pKa = 9.93 PWCAGIEE222 pKa = 4.21 SGDD225 pKa = 3.58 ATGWPATDD233 pKa = 3.11 WLEE236 pKa = 4.41 DD237 pKa = 3.59 VVLRR241 pKa = 11.84 TAGPDD246 pKa = 4.24 VYY248 pKa = 10.79 DD249 pKa = 2.82 QWVNHH254 pKa = 7.0 EE255 pKa = 4.78 IPFDD259 pKa = 4.16 DD260 pKa = 4.3 PQIVAALEE268 pKa = 3.92 EE269 pKa = 4.33 AGGILKK275 pKa = 10.62 NEE277 pKa = 4.49 DD278 pKa = 3.73 YY279 pKa = 11.83 VNGGLGNVEE288 pKa = 5.09 SIATAPWTEE297 pKa = 3.78 AGLPVIDD304 pKa = 5.06 GSCFLHH310 pKa = 6.93 RR311 pKa = 11.84 AANFYY316 pKa = 10.49 AANLEE321 pKa = 4.3 GYY323 pKa = 9.09 EE324 pKa = 4.05 VAEE327 pKa = 4.95 DD328 pKa = 4.1 GEE330 pKa = 4.45 IFAFYY335 pKa = 10.71 LPGPTEE341 pKa = 4.08 DD342 pKa = 4.21 EE343 pKa = 4.35 KK344 pKa = 11.35 PLLGGGEE351 pKa = 4.35 FVTAFSDD358 pKa = 3.76 RR359 pKa = 11.84 PEE361 pKa = 3.99 VQAFHH366 pKa = 7.45 AYY368 pKa = 10.19 LSSPQWANAKK378 pKa = 10.37 AEE380 pKa = 4.23 STPGGGWLSANSGLDD395 pKa = 3.26 RR396 pKa = 11.84 DD397 pKa = 4.84 LLTSPIDD404 pKa = 3.45 QLSFDD409 pKa = 5.11 LLTDD413 pKa = 3.55 DD414 pKa = 5.54 AYY416 pKa = 9.96 TFRR419 pKa = 11.84 FDD421 pKa = 5.99 GSDD424 pKa = 3.49 QMPGAVGAGAFWRR437 pKa = 11.84 EE438 pKa = 3.62 MTAWISPGQDD448 pKa = 2.75 AQTTLTNIEE457 pKa = 4.31 AAWPTSS463 pKa = 3.27
Molecular weight: 49.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.834
Patrickios 0.757
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A4P7SLV9|A0A4P7SLV9_9CELL ArsR family transcriptional regulator OS=Cellulomonas shaoxiangyii OX=2566013 GN=E5225_10215 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3337
0
3337
1147658
29
2544
343.9
36.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.224 ± 0.068
0.553 ± 0.01
6.6 ± 0.033
4.992 ± 0.042
2.341 ± 0.026
9.67 ± 0.039
2.163 ± 0.019
2.303 ± 0.033
1.15 ± 0.024
10.244 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.445 ± 0.016
1.375 ± 0.021
6.318 ± 0.036
2.534 ± 0.022
8.279 ± 0.043
4.407 ± 0.026
6.391 ± 0.042
10.632 ± 0.045
1.611 ± 0.019
1.769 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here