Natronomonas sp. CBA1123

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Natronomonas; unclassified Natronomonas

Average proteome isoelectric point is 4.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A9SSB6|A0A6A9SSB6_9EURY Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein OS=Natronomonas sp. CBA1123 OX=2668070 GN=GJ631_15660 PE=3 SV=1
MM1 pKa = 7.52LSEE4 pKa = 4.42GTTAPDD10 pKa = 3.48FTLSGLGLPNDD21 pKa = 3.67ADD23 pKa = 3.74EE24 pKa = 5.06RR25 pKa = 11.84RR26 pKa = 11.84MTEE29 pKa = 3.58YY30 pKa = 10.51HH31 pKa = 6.38LEE33 pKa = 3.96EE34 pKa = 4.6FARR37 pKa = 11.84GTPTLLAFYY46 pKa = 9.44PGDD49 pKa = 3.92FSPVCTDD56 pKa = 3.49EE57 pKa = 4.73LCSLRR62 pKa = 11.84DD63 pKa = 3.5IALSAIDD70 pKa = 3.71TSRR73 pKa = 11.84DD74 pKa = 3.26VYY76 pKa = 11.08GISRR80 pKa = 11.84DD81 pKa = 3.49SLFTHH86 pKa = 6.49EE87 pKa = 4.9AFAYY91 pKa = 10.46DD92 pKa = 3.73HH93 pKa = 7.47DD94 pKa = 4.36IDD96 pKa = 5.24FPLLSDD102 pKa = 4.28VEE104 pKa = 4.45GDD106 pKa = 3.21VCAAYY111 pKa = 10.07DD112 pKa = 4.42AIHH115 pKa = 6.82EE116 pKa = 4.25EE117 pKa = 4.16DD118 pKa = 3.95AGDD121 pKa = 3.76GVEE124 pKa = 4.75AGLAKK129 pKa = 9.59RR130 pKa = 11.84TLYY133 pKa = 10.84VLAPDD138 pKa = 4.13LTIEE142 pKa = 4.34YY143 pKa = 9.53AWQSDD148 pKa = 4.26DD149 pKa = 3.51PWVDD153 pKa = 3.31PDD155 pKa = 3.36IEE157 pKa = 4.27AVVDD161 pKa = 3.75EE162 pKa = 4.98LVAAGAA168 pKa = 3.99

Molecular weight: 18.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A9SKX2|A0A6A9SKX2_9EURY UPF0215 protein GJ631_03140 OS=Natronomonas sp. CBA1123 OX=2668070 GN=GJ631_03140 PE=3 SV=1
MM1 pKa = 7.36VSVLGEE7 pKa = 3.93APGARR12 pKa = 11.84RR13 pKa = 11.84PHH15 pKa = 5.87TAGGPQHH22 pKa = 6.74RR23 pKa = 11.84RR24 pKa = 11.84GPRR27 pKa = 11.84GGSVQAPRR35 pKa = 11.84SGFEE39 pKa = 3.94SARR42 pKa = 11.84GQQARR47 pKa = 11.84EE48 pKa = 3.57EE49 pKa = 4.24WEE51 pKa = 3.74RR52 pKa = 11.84RR53 pKa = 11.84QRR55 pKa = 11.84QQRR58 pKa = 11.84QRR60 pKa = 11.84QQQKK64 pKa = 7.86RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84ADD70 pKa = 3.29VGPSPGLSRR79 pKa = 11.84AEE81 pKa = 3.88AYY83 pKa = 9.62RR84 pKa = 11.84RR85 pKa = 11.84LGLDD89 pKa = 2.94TDD91 pKa = 3.94ADD93 pKa = 3.6EE94 pKa = 5.72GEE96 pKa = 4.18IKK98 pKa = 10.14RR99 pKa = 11.84AYY101 pKa = 9.95RR102 pKa = 11.84EE103 pKa = 4.18RR104 pKa = 11.84IKK106 pKa = 10.43EE107 pKa = 3.93AHH109 pKa = 7.25PDD111 pKa = 3.34RR112 pKa = 11.84GGDD115 pKa = 3.17EE116 pKa = 5.25AEE118 pKa = 4.48FKK120 pKa = 10.72RR121 pKa = 11.84LTEE124 pKa = 4.29AYY126 pKa = 7.38EE127 pKa = 4.23TLTEE131 pKa = 4.05

Molecular weight: 14.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3455

0

3455

969895

23

1855

280.7

30.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.676 ± 0.049

0.745 ± 0.014

8.174 ± 0.058

8.903 ± 0.07

3.459 ± 0.032

8.609 ± 0.046

1.963 ± 0.018

4.115 ± 0.035

1.865 ± 0.027

8.966 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.017

2.423 ± 0.025

4.68 ± 0.028

2.417 ± 0.023

6.414 ± 0.042

5.497 ± 0.034

6.41 ± 0.037

9.063 ± 0.048

1.088 ± 0.016

2.769 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski