Aleutian mink disease parvovirus (strain G) (ADV)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P24029-2|CAPSD-2_ADVG Isoform of P24029 Isoform VP2 of Capsid protein VP1 OS=Aleutian mink disease parvovirus (strain G) OX=10783 PE=4 SV=3
MM1 pKa = 7.61 DD2 pKa = 4.39 TEE4 pKa = 4.06 QATNQTAEE12 pKa = 4.04 AGGGGGGGGGGGGGGGGVGNSTGGFNNTTEE42 pKa = 4.13 FKK44 pKa = 10.82 VINNEE49 pKa = 4.17 VYY51 pKa = 8.79 ITCHH55 pKa = 5.26 ATRR58 pKa = 11.84 MVHH61 pKa = 6.78 INQADD66 pKa = 3.58 TDD68 pKa = 3.77 EE69 pKa = 4.15 YY70 pKa = 11.37 LIFNAGRR77 pKa = 11.84 TTDD80 pKa = 3.58 TKK82 pKa = 10.25 THH84 pKa = 4.52 QQKK87 pKa = 10.94 LNLEE91 pKa = 4.21 FFVYY95 pKa = 10.6 DD96 pKa = 4.2 DD97 pKa = 3.63 FHH99 pKa = 7.41 QQVMTPWYY107 pKa = 9.87 IVDD110 pKa = 3.74 SNAWGVWMSPKK121 pKa = 10.4 DD122 pKa = 3.74 FQQMKK127 pKa = 8.04 TLCSEE132 pKa = 4.05 ISLVTLEE139 pKa = 4.18 QEE141 pKa = 3.92 IDD143 pKa = 3.25 NVTIKK148 pKa = 9.97 TVTEE152 pKa = 4.31 TNQGNASTKK161 pKa = 10.09 QFNNDD166 pKa = 2.95 LTASLQVALDD176 pKa = 3.76 TNNILPYY183 pKa = 10.23 TPAAPLGEE191 pKa = 4.19 TLGFVPWRR199 pKa = 11.84 ATKK202 pKa = 8.31 PTQYY206 pKa = 10.37 RR207 pKa = 11.84 YY208 pKa = 9.54 YY209 pKa = 9.47 HH210 pKa = 6.5 PCYY213 pKa = 10.07 IYY215 pKa = 10.9 NRR217 pKa = 11.84 YY218 pKa = 9.47 PNIQKK223 pKa = 10.0 VATEE227 pKa = 4.13 TLTWDD232 pKa = 3.81 AVQDD236 pKa = 4.13 DD237 pKa = 4.76 YY238 pKa = 12.2 LSVDD242 pKa = 3.22 EE243 pKa = 5.11 QYY245 pKa = 11.6 FNFITIEE252 pKa = 3.84 NNIPINILRR261 pKa = 11.84 TGDD264 pKa = 3.51 NFHH267 pKa = 6.66 TGLYY271 pKa = 9.22 EE272 pKa = 4.78 FNSKK276 pKa = 9.35 PCKK279 pKa = 9.38 LTLSYY284 pKa = 11.09 QSTRR288 pKa = 11.84 CLGLPPLCKK297 pKa = 9.94 PKK299 pKa = 10.35 TDD301 pKa = 3.56 TTHH304 pKa = 7.33 KK305 pKa = 9.74 VTSKK309 pKa = 11.17 EE310 pKa = 3.79 NGADD314 pKa = 4.09 LIYY317 pKa = 10.59 IQGQDD322 pKa = 3.31 NTRR325 pKa = 11.84 LGHH328 pKa = 5.84 FWGEE332 pKa = 3.7 EE333 pKa = 3.69 RR334 pKa = 11.84 GKK336 pKa = 11.06 KK337 pKa = 7.95 NAEE340 pKa = 3.93 MNRR343 pKa = 11.84 IRR345 pKa = 11.84 PYY347 pKa = 10.72 NIGYY351 pKa = 9.48 QYY353 pKa = 10.45 PEE355 pKa = 4.2 WIIPAGLQGSYY366 pKa = 10.22 FAGGPRR372 pKa = 11.84 QWSDD376 pKa = 3.02 TTKK379 pKa = 10.85 GAGTHH384 pKa = 5.4 SQHH387 pKa = 5.97 LQQNFSTRR395 pKa = 11.84 YY396 pKa = 9.3 IYY398 pKa = 10.51 DD399 pKa = 3.62 RR400 pKa = 11.84 NHH402 pKa = 6.48 GGDD405 pKa = 3.75 NEE407 pKa = 4.03 VDD409 pKa = 3.96 LLDD412 pKa = 5.26 GIPIHH417 pKa = 6.38 EE418 pKa = 4.43 RR419 pKa = 11.84 SNYY422 pKa = 9.77 YY423 pKa = 10.19 SDD425 pKa = 5.18 NEE427 pKa = 4.33 IEE429 pKa = 4.14 QHH431 pKa = 5.1 TAKK434 pKa = 10.19 QPKK437 pKa = 9.71 LRR439 pKa = 11.84 TPPIHH444 pKa = 6.73 HH445 pKa = 6.74 SKK447 pKa = 9.66 IDD449 pKa = 3.27 SWEE452 pKa = 4.09 EE453 pKa = 3.79 EE454 pKa = 4.21 GWPAASGTHH463 pKa = 6.32 FEE465 pKa = 5.02 DD466 pKa = 3.61 EE467 pKa = 4.8 VIYY470 pKa = 10.85 LDD472 pKa = 3.58 YY473 pKa = 11.23 FNFSGEE479 pKa = 4.03 QEE481 pKa = 4.33 LNFPHH486 pKa = 6.93 EE487 pKa = 4.47 VLDD490 pKa = 5.59 DD491 pKa = 3.87 AAQMKK496 pKa = 10.23 KK497 pKa = 10.43 LLNSYY502 pKa = 10.11 QPTVAQDD509 pKa = 3.57 NVGPVYY515 pKa = 10.22 PWGQIWDD522 pKa = 4.16 KK523 pKa = 10.56 KK524 pKa = 9.64 PHH526 pKa = 6.31 MDD528 pKa = 4.78 HH529 pKa = 6.78 KK530 pKa = 11.06 PSMNNNAPFVCKK542 pKa = 10.44 NNPPGQLFVKK552 pKa = 9.57 LTEE555 pKa = 4.26 NLTDD559 pKa = 3.34 TFNYY563 pKa = 10.31 DD564 pKa = 3.53 EE565 pKa = 4.8 NPDD568 pKa = 3.81 RR569 pKa = 11.84 IKK571 pKa = 10.22 TYY573 pKa = 10.59 GYY575 pKa = 7.33 FTWRR579 pKa = 11.84 GKK581 pKa = 10.53 LVLKK585 pKa = 10.79 GKK587 pKa = 10.28 LSQVTCWNPVKK598 pKa = 10.52 RR599 pKa = 11.84 EE600 pKa = 4.15 LIGEE604 pKa = 4.24 PGVFTKK610 pKa = 10.59 DD611 pKa = 3.42 KK612 pKa = 9.94 YY613 pKa = 10.84 HH614 pKa = 6.71 KK615 pKa = 10.18 QIPNNKK621 pKa = 8.58 GNFEE625 pKa = 4.05 IGLQYY630 pKa = 10.86 GRR632 pKa = 11.84 STIKK636 pKa = 10.69 YY637 pKa = 8.68 IYY639 pKa = 10.37
Molecular weight: 72.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.412
IPC2_protein 5.423
IPC_protein 5.461
Toseland 5.817
ProMoST 5.753
Dawson 5.652
Bjellqvist 5.69
Wikipedia 5.639
Rodwell 5.626
Grimsley 5.957
Solomon 5.652
Lehninger 5.639
Nozaki 5.893
DTASelect 6.071
Thurlkill 6.033
EMBOSS 5.995
Sillero 5.982
Patrickios 3.63
IPC_peptide 5.664
IPC2_peptide 5.995
IPC2.peptide.svr19 5.931
Protein with the highest isoelectric point:
>sp|P24029-2|CAPSD-2_ADVG Isoform of P24029 Isoform VP2 of Capsid protein VP1 OS=Aleutian mink disease parvovirus (strain G) OX=10783 PE=4 SV=3
MM1 pKa = 7.37 AQAQIDD7 pKa = 4.1 EE8 pKa = 4.16 QRR10 pKa = 11.84 RR11 pKa = 11.84 LQDD14 pKa = 4.46 LYY16 pKa = 11.42 VQLKK20 pKa = 10.4 KK21 pKa = 10.66 EE22 pKa = 4.2 INDD25 pKa = 4.01 GEE27 pKa = 4.53 GVAWLFQQKK36 pKa = 8.97 TYY38 pKa = 9.6 TDD40 pKa = 4.0 KK41 pKa = 11.47 DD42 pKa = 3.7 NKK44 pKa = 7.85 PTKK47 pKa = 9.25 ATPPLRR53 pKa = 11.84 TTSSDD58 pKa = 5.26 LRR60 pKa = 11.84 LCDD63 pKa = 4.07 CNKK66 pKa = 9.53 QHH68 pKa = 6.49 QHH70 pKa = 5.05 NQSNCWMCGNKK81 pKa = 9.57 RR82 pKa = 11.84 SRR84 pKa = 11.84 RR85 pKa = 11.84 ATT87 pKa = 3.29
Molecular weight: 10.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.62
IPC2_protein 8.361
IPC_protein 8.361
Toseland 8.829
ProMoST 8.814
Dawson 9.165
Bjellqvist 9.238
Wikipedia 9.194
Rodwell 9.326
Grimsley 8.668
Solomon 9.399
Lehninger 9.399
Nozaki 9.545
DTASelect 9.033
Thurlkill 9.18
EMBOSS 9.37
Sillero 9.414
Patrickios 5.194
IPC_peptide 9.385
IPC2_peptide 8.17
IPC2.peptide.svr19 7.846
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
1
5
2120
87
690
424.0
48.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.528 ± 0.204
2.075 ± 0.52
6.321 ± 0.408
5.755 ± 0.364
3.443 ± 0.316
7.264 ± 0.763
2.83 ± 0.321
5.566 ± 0.428
7.972 ± 1.14
6.934 ± 0.509
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.745 ± 0.216
7.217 ± 0.378
5.0 ± 0.426
6.085 ± 0.447
3.396 ± 0.328
4.151 ± 0.127
8.679 ± 0.087
4.245 ± 0.223
2.217 ± 0.078
4.575 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here