Streptomyces sp. ERV7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A197SNY5|A0A197SNY5_9ACTN Epimerase OS=Streptomyces sp. ERV7 OX=1322334 GN=A8W25_19700 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.84EE3 pKa = 3.9ICVPLYY9 pKa = 11.11VEE11 pKa = 4.92LLHH14 pKa = 6.88EE15 pKa = 5.58GGDD18 pKa = 3.31AMTPVEE24 pKa = 5.29AVMTYY29 pKa = 11.06DD30 pKa = 3.95SDD32 pKa = 4.63DD33 pKa = 3.96PLAVSVDD40 pKa = 4.31FVCGNGTHH48 pKa = 5.81TTWTMGRR55 pKa = 11.84DD56 pKa = 3.51LVAEE60 pKa = 4.46GLTSRR65 pKa = 11.84RR66 pKa = 11.84PVGMGDD72 pKa = 3.21VRR74 pKa = 11.84VWYY77 pKa = 10.11CGEE80 pKa = 3.87GGLRR84 pKa = 11.84IRR86 pKa = 11.84LDD88 pKa = 3.54SDD90 pKa = 3.21EE91 pKa = 4.82GSADD95 pKa = 3.59LLAGLEE101 pKa = 4.24DD102 pKa = 3.46VAEE105 pKa = 4.27FLSRR109 pKa = 11.84TYY111 pKa = 11.01QLVPEE116 pKa = 4.59GSEE119 pKa = 3.82LDD121 pKa = 4.11GYY123 pKa = 11.33DD124 pKa = 3.05VDD126 pKa = 5.59AVLDD130 pKa = 4.08LLFAPTPLPAPACRR144 pKa = 11.84LCGRR148 pKa = 11.84EE149 pKa = 4.6CPCLDD154 pKa = 4.48HH155 pKa = 7.27GAQQ158 pKa = 3.43

Molecular weight: 17.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A197SLX7|A0A197SLX7_9ACTN Uncharacterized protein OS=Streptomyces sp. ERV7 OX=1322334 GN=A8W25_15375 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5791

0

5791

1884294

36

3635

325.4

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.587 ± 0.051

0.811 ± 0.008

5.914 ± 0.021

5.63 ± 0.033

2.8 ± 0.017

9.438 ± 0.034

2.33 ± 0.017

3.156 ± 0.02

2.376 ± 0.029

10.344 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.78 ± 0.012

1.838 ± 0.02

5.903 ± 0.028

2.82 ± 0.02

7.9 ± 0.041

5.116 ± 0.024

6.137 ± 0.035

8.403 ± 0.031

1.53 ± 0.015

2.186 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski