Canine coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Tegacovirus; Alphacoronavirus 1

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9TEX6|H9TEX6_9ALPC Membrane protein OS=Canine coronavirus OX=11153 GN=M PE=3 SV=1
MM1 pKa = 7.4IVLVTCILLLCSYY14 pKa = 9.04HH15 pKa = 5.95TVLSTANNDD24 pKa = 3.13CRR26 pKa = 11.84QVNVTQLPGNEE37 pKa = 3.63YY38 pKa = 10.62LIRR41 pKa = 11.84DD42 pKa = 3.96FLFQSFKK49 pKa = 11.11EE50 pKa = 4.16EE51 pKa = 4.09GSVVVGGYY59 pKa = 10.2YY60 pKa = 8.53PTEE63 pKa = 3.31VWYY66 pKa = 10.62NCSRR70 pKa = 11.84TATTTAYY77 pKa = 10.43QYY79 pKa = 11.1FNNIHH84 pKa = 6.68AFYY87 pKa = 10.77FDD89 pKa = 3.66MEE91 pKa = 4.25AMEE94 pKa = 4.6NSTGNARR101 pKa = 11.84GKK103 pKa = 9.27PLLFHH108 pKa = 5.86VHH110 pKa = 6.68GDD112 pKa = 3.65PVSVIIYY119 pKa = 9.4ISAYY123 pKa = 9.94RR124 pKa = 11.84DD125 pKa = 3.89DD126 pKa = 4.15VQHH129 pKa = 7.2RR130 pKa = 11.84PLLKK134 pKa = 10.4HH135 pKa = 6.16GLVCITKK142 pKa = 10.19SRR144 pKa = 11.84NIDD147 pKa = 3.41YY148 pKa = 7.88NTFTSSQWNSICTGNDD164 pKa = 2.5RR165 pKa = 11.84KK166 pKa = 10.45IPFSVIPTDD175 pKa = 3.44NGTKK179 pKa = 9.62IYY181 pKa = 10.47GLEE184 pKa = 4.03WNDD187 pKa = 3.62EE188 pKa = 4.1FVTAYY193 pKa = 9.89ISGHH197 pKa = 5.06SYY199 pKa = 10.18NWNINNNWFNNVTLLYY215 pKa = 10.18SRR217 pKa = 11.84SSTATWQHH225 pKa = 4.71SAAYY229 pKa = 9.59VYY231 pKa = 10.56QGVSNFTYY239 pKa = 10.84YY240 pKa = 11.24KK241 pKa = 10.71LNNTNGLKK249 pKa = 9.77TYY251 pKa = 7.25EE252 pKa = 3.93FCEE255 pKa = 4.49DD256 pKa = 3.71YY257 pKa = 10.67EE258 pKa = 4.64YY259 pKa = 10.83CTATATNVFAPTVGGYY275 pKa = 9.69IPDD278 pKa = 3.64GFSFNNWFLLTNSSTFVSGRR298 pKa = 11.84FVTNQPLLVNCLWPVPSFGVAAQEE322 pKa = 4.3FCFEE326 pKa = 4.3GAQFSQCNGMSLNNTVDD343 pKa = 3.78VIRR346 pKa = 11.84FNLNFTADD354 pKa = 3.63VQSGMGATVFSLNTTGGVILEE375 pKa = 4.07ISCYY379 pKa = 9.9KK380 pKa = 10.17DD381 pKa = 3.22TVSEE385 pKa = 4.31SSFYY389 pKa = 10.93SYY391 pKa = 11.79GEE393 pKa = 3.88IPFGITDD400 pKa = 4.33GPRR403 pKa = 11.84YY404 pKa = 10.15CYY406 pKa = 10.84VLYY409 pKa = 10.96NGTALKK415 pKa = 10.82YY416 pKa = 10.58LGTLPPSVKK425 pKa = 10.2EE426 pKa = 3.64IAISKK431 pKa = 8.76WGHH434 pKa = 5.86FYY436 pKa = 10.78INGYY440 pKa = 9.41NFFSTFPIDD449 pKa = 4.28CISFNLTTGASGAFWTIAYY468 pKa = 7.76TSYY471 pKa = 11.12TEE473 pKa = 5.09ALVQVEE479 pKa = 4.01NTAIKK484 pKa = 10.3KK485 pKa = 7.68VTYY488 pKa = 10.28CNSHH492 pKa = 6.35INNIKK497 pKa = 10.33CSQLTANLNNGFYY510 pKa = 10.08PVASSEE516 pKa = 4.07VGLVNKK522 pKa = 10.31SVVLLPTFFAHH533 pKa = 5.84TAFNITIDD541 pKa = 3.66LGMKK545 pKa = 10.04RR546 pKa = 11.84SGYY549 pKa = 8.22GQPIASTLSNITLPMQDD566 pKa = 3.7NNTDD570 pKa = 3.69VYY572 pKa = 10.5CVRR575 pKa = 11.84SNQFSVYY582 pKa = 8.11VHH584 pKa = 5.76STCKK588 pKa = 10.33SSSWDD593 pKa = 3.38NIFNQDD599 pKa = 3.41CTDD602 pKa = 3.44VLEE605 pKa = 4.39ATAVIKK611 pKa = 9.94TGTCPFSFDD620 pKa = 3.91KK621 pKa = 11.18LNNYY625 pKa = 7.76LTFNKK630 pKa = 9.63FCLSLSPVGANCKK643 pKa = 9.97FDD645 pKa = 3.74VAAHH649 pKa = 5.81TRR651 pKa = 11.84TNEE654 pKa = 3.69QVVRR658 pKa = 11.84SLYY661 pKa = 10.45VIYY664 pKa = 10.55EE665 pKa = 4.03EE666 pKa = 4.75GDD668 pKa = 3.7NIVGVPSDD676 pKa = 3.48NSGLHH681 pKa = 6.34DD682 pKa = 5.3LSVLHH687 pKa = 7.02LDD689 pKa = 3.23SCTDD693 pKa = 3.23YY694 pKa = 11.33NIYY697 pKa = 10.59GRR699 pKa = 11.84TGVGIIRR706 pKa = 11.84QTNSTLLSGLYY717 pKa = 8.14YY718 pKa = 10.47TSLSGDD724 pKa = 3.35LLGFKK729 pKa = 10.1NVSDD733 pKa = 3.82GVIYY737 pKa = 10.67SVTPCDD743 pKa = 3.31VSAQVAVIDD752 pKa = 4.2GAIVGAMTSINSEE765 pKa = 4.32LLGLTHH771 pKa = 7.13WTTTPNFYY779 pKa = 10.7YY780 pKa = 10.66YY781 pKa = 10.66SIYY784 pKa = 10.78NYY786 pKa = 9.49TNEE789 pKa = 4.22RR790 pKa = 11.84TRR792 pKa = 11.84GTAIDD797 pKa = 4.46SNDD800 pKa = 3.38VDD802 pKa = 4.54CEE804 pKa = 4.34PIITYY809 pKa = 11.09ANIGVCKK816 pKa = 10.5NGALVFINVTHH827 pKa = 7.03SDD829 pKa = 3.31GDD831 pKa = 3.76VQPISTGNVTIPTNFTISVQVEE853 pKa = 4.08YY854 pKa = 10.31IQVYY858 pKa = 5.57TTPVSIDD865 pKa = 3.21CSRR868 pKa = 11.84YY869 pKa = 8.47VCNGNPRR876 pKa = 11.84CNKK879 pKa = 10.11LLTQYY884 pKa = 10.84VSACQTIEE892 pKa = 3.6QALAMGARR900 pKa = 11.84LEE902 pKa = 4.0NMEE905 pKa = 4.38VDD907 pKa = 3.28SMLFVSEE914 pKa = 4.35NALKK918 pKa = 10.36LASVEE923 pKa = 4.2AFNSTEE929 pKa = 3.91NLDD932 pKa = 4.31PIYY935 pKa = 10.67KK936 pKa = 9.41EE937 pKa = 3.84WPNIGGSWLGGLKK950 pKa = 10.5DD951 pKa = 4.17ILPSHH956 pKa = 5.68NSKK959 pKa = 10.42RR960 pKa = 11.84KK961 pKa = 8.03YY962 pKa = 10.0RR963 pKa = 11.84SAIEE967 pKa = 4.79DD968 pKa = 3.77LLFDD972 pKa = 3.96KK973 pKa = 11.1VVTSGLGTVDD983 pKa = 3.79EE984 pKa = 4.94DD985 pKa = 4.2YY986 pKa = 11.18KK987 pKa = 11.08RR988 pKa = 11.84CTGGYY993 pKa = 10.17DD994 pKa = 3.45IADD997 pKa = 4.27LVCAQYY1003 pKa = 11.58YY1004 pKa = 8.99NGIMVLPGVANDD1016 pKa = 5.23DD1017 pKa = 3.64KK1018 pKa = 10.09MTMYY1022 pKa = 9.25TASLAGGITLGALGGGAVAIPFAVAVQARR1051 pKa = 11.84LNYY1054 pKa = 9.72VALQTDD1060 pKa = 4.12VLNKK1064 pKa = 9.83NQQILANAFNQAIGNITQAFGKK1086 pKa = 10.65VNDD1089 pKa = 5.6AIHH1092 pKa = 5.36QTSQGLATVAKK1103 pKa = 10.13ALAKK1107 pKa = 10.36VQDD1110 pKa = 3.83VVNTQGQALSHH1121 pKa = 6.03LTVQLQNNFQAISSSISDD1139 pKa = 3.33IYY1141 pKa = 11.39NRR1143 pKa = 11.84LDD1145 pKa = 3.61EE1146 pKa = 4.93LSADD1150 pKa = 3.68AQVDD1154 pKa = 3.66RR1155 pKa = 11.84LITGRR1160 pKa = 11.84LTALNAFVSQTLTRR1174 pKa = 11.84QAEE1177 pKa = 4.39VRR1179 pKa = 11.84ASRR1182 pKa = 11.84QLAKK1186 pKa = 10.86DD1187 pKa = 3.4KK1188 pKa = 11.38VNEE1191 pKa = 4.27CVRR1194 pKa = 11.84SQSQRR1199 pKa = 11.84FGFCGNGTHH1208 pKa = 7.49LFSLANAAPNGMIFFHH1224 pKa = 6.14TVLLPTAYY1232 pKa = 8.01EE1233 pKa = 4.33TVTAWSGICASDD1245 pKa = 3.56GDD1247 pKa = 4.1RR1248 pKa = 11.84TFGLVVKK1255 pKa = 10.13DD1256 pKa = 3.57VQLTLFRR1263 pKa = 11.84NLDD1266 pKa = 3.49GKK1268 pKa = 11.0FYY1270 pKa = 9.4LTPRR1274 pKa = 11.84TMYY1277 pKa = 9.68QPRR1280 pKa = 11.84VVTSSDD1286 pKa = 3.09FVQIEE1291 pKa = 4.46GCDD1294 pKa = 3.54VLFVNATVIDD1304 pKa = 4.59LPSIIPDD1311 pKa = 3.84YY1312 pKa = 11.09IDD1314 pKa = 3.75INQTVQDD1321 pKa = 3.37ILEE1324 pKa = 4.31NYY1326 pKa = 8.5RR1327 pKa = 11.84PNWTVPEE1334 pKa = 4.11LTLDD1338 pKa = 3.76IFNATYY1344 pKa = 11.19LNLTGEE1350 pKa = 4.13IDD1352 pKa = 3.65DD1353 pKa = 5.06LEE1355 pKa = 4.33FRR1357 pKa = 11.84SEE1359 pKa = 4.1KK1360 pKa = 10.53LHH1362 pKa = 5.34NTTVEE1367 pKa = 3.84LAILIDD1373 pKa = 4.43NINNTLVNLEE1383 pKa = 3.92WLNRR1387 pKa = 11.84IEE1389 pKa = 5.6TYY1391 pKa = 10.75VKK1393 pKa = 9.1WPWYY1397 pKa = 8.39VWLLIGLVVIFCIPLLLFCCCSTGCCGCIGCLGSCCHH1434 pKa = 6.85SICSRR1439 pKa = 11.84RR1440 pKa = 11.84QFEE1443 pKa = 4.2NYY1445 pKa = 9.36EE1446 pKa = 4.38PIEE1449 pKa = 4.26KK1450 pKa = 10.17VHH1452 pKa = 5.03VHH1454 pKa = 6.07

Molecular weight: 161.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9TEX3|H9TEX3_9ALPC 3C OS=Canine coronavirus OX=11153 PE=4 SV=1
MM1 pKa = 7.51LSLVSPLLKK10 pKa = 10.35KK11 pKa = 10.86SIVIQLFNITVYY23 pKa = 10.74KK24 pKa = 10.02FQAKK28 pKa = 8.76FWYY31 pKa = 9.49KK32 pKa = 10.81LPFEE36 pKa = 4.4TRR38 pKa = 11.84LCIIKK43 pKa = 8.63HH44 pKa = 5.46TKK46 pKa = 9.52PKK48 pKa = 10.45ALSATKK54 pKa = 9.69QVKK57 pKa = 9.41RR58 pKa = 11.84DD59 pKa = 3.56YY60 pKa = 10.98RR61 pKa = 11.84KK62 pKa = 9.79AAILNSMRR70 pKa = 11.84KK71 pKa = 9.08

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

13608

71

6685

1237.1

138.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.481 ± 0.177

3.094 ± 0.282

5.409 ± 0.252

5.049 ± 0.398

5.489 ± 0.18

6.32 ± 0.145

1.808 ± 0.173

5.989 ± 0.268

6.43 ± 0.808

8.392 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.304

5.938 ± 0.442

3.241 ± 0.1

3.057 ± 0.377

3.123 ± 0.47

6.922 ± 0.267

5.938 ± 0.552

9.053 ± 0.388

1.161 ± 0.113

4.769 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski