Amino acid dipepetide frequency for Rugamonas rubra

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.637AlaAla: 22.637 ± 0.215	1.253AlaCys: 1.253 ± 0.026	7.276AlaAsp: 7.276 ± 0.07	7.257AlaGlu: 7.257 ± 0.074	3.792AlaPhe: 3.792 ± 0.047	12.463AlaGly: 12.463 ± 0.147	2.431AlaHis: 2.431 ± 0.037	5.417AlaIle: 5.417 ± 0.057	4.445AlaLys: 4.445 ± 0.073	16.268AlaLeu: 16.268 ± 0.174	3.418AlaMet: 3.418 ± 0.043	3.464AlaAsn: 3.464 ± 0.05	7.276AlaPro: 7.276 ± 0.094	5.682AlaGln: 5.682 ± 0.082	8.711AlaArg: 8.711 ± 0.085	7.155AlaSer: 7.155 ± 0.075	6.042AlaThr: 6.042 ± 0.066	8.863AlaVal: 8.863 ± 0.075	1.656AlaTrp: 1.656 ± 0.031	2.787AlaTyr: 2.787 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.308CysAla: 1.308 ± 0.026	0.136CysCys: 0.136 ± 0.01	0.527CysAsp: 0.527 ± 0.017	0.427CysGlu: 0.427 ± 0.015	0.302CysPhe: 0.302 ± 0.012	0.927CysGly: 0.927 ± 0.024	0.245CysHis: 0.245 ± 0.012	0.342CysIle: 0.342 ± 0.014	0.242CysLys: 0.242 ± 0.012	0.88CysLeu: 0.88 ± 0.022	0.195CysMet: 0.195 ± 0.011	0.25CysAsn: 0.25 ± 0.012	0.41CysPro: 0.41 ± 0.016	0.308CysGln: 0.308 ± 0.013	0.566CysArg: 0.566 ± 0.019	0.473CysSer: 0.473 ± 0.017	0.377CysThr: 0.377 ± 0.014	0.655CysVal: 0.655 ± 0.018	0.137CysTrp: 0.137 ± 0.009	0.234CysTyr: 0.234 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.88AspAla: 6.88 ± 0.07	0.525AspCys: 0.525 ± 0.017	2.789AspAsp: 2.789 ± 0.042	2.978AspGlu: 2.978 ± 0.045	2.09AspPhe: 2.09 ± 0.036	5.391AspGly: 5.391 ± 0.062	0.997AspHis: 0.997 ± 0.024	2.538AspIle: 2.538 ± 0.033	2.022AspLys: 2.022 ± 0.036	5.055AspLeu: 5.055 ± 0.063	1.264AspMet: 1.264 ± 0.027	1.583AspAsn: 1.583 ± 0.037	2.597AspPro: 2.597 ± 0.039	1.968AspGln: 1.968 ± 0.058	2.637AspArg: 2.637 ± 0.038	2.723AspSer: 2.723 ± 0.038	2.487AspThr: 2.487 ± 0.037	3.467AspVal: 3.467 ± 0.046	0.95AspTrp: 0.95 ± 0.017	1.602AspTyr: 1.602 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.299GluAla: 6.299 ± 0.066	0.338GluCys: 0.338 ± 0.012	1.84GluAsp: 1.84 ± 0.034	2.337GluGlu: 2.337 ± 0.045	1.786GluPhe: 1.786 ± 0.029	3.122GluGly: 3.122 ± 0.051	1.41GluHis: 1.41 ± 0.03	2.566GluIle: 2.566 ± 0.044	1.771GluLys: 1.771 ± 0.037	6.504GluLeu: 6.504 ± 0.073	1.252GluMet: 1.252 ± 0.025	1.272GluAsn: 1.272 ± 0.028	2.174GluPro: 2.174 ± 0.035	3.394GluGln: 3.394 ± 0.055	4.428GluArg: 4.428 ± 0.053	2.206GluSer: 2.206 ± 0.033	2.247GluThr: 2.247 ± 0.037	3.584GluVal: 3.584 ± 0.051	0.666GluTrp: 0.666 ± 0.017	1.079GluTyr: 1.079 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.285PheAla: 4.285 ± 0.046	0.386PheCys: 0.386 ± 0.016	2.452PheAsp: 2.452 ± 0.042	1.823PheGlu: 1.823 ± 0.03	1.347PhePhe: 1.347 ± 0.03	3.316PheGly: 3.316 ± 0.046	0.753PheHis: 0.753 ± 0.02	1.527PheIle: 1.527 ± 0.03	1.299PheLys: 1.299 ± 0.026	3.003PheLeu: 3.003 ± 0.045	0.771PheMet: 0.771 ± 0.019	1.258PheAsn: 1.258 ± 0.023	1.41PhePro: 1.41 ± 0.025	1.152PheGln: 1.152 ± 0.026	1.781PheArg: 1.781 ± 0.034	2.357PheSer: 2.357 ± 0.037	1.74PheThr: 1.74 ± 0.032	2.501PheVal: 2.501 ± 0.035	0.436PheTrp: 0.436 ± 0.015	0.965PheTyr: 0.965 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	11.323GlyAla: 11.323 ± 0.133	0.773GlyCys: 0.773 ± 0.022	3.986GlyAsp: 3.986 ± 0.058	4.188GlyGlu: 4.188 ± 0.049	3.091GlyPhe: 3.091 ± 0.04	7.577GlyGly: 7.577 ± 0.109	1.893GlyHis: 1.893 ± 0.032	3.658GlyIle: 3.658 ± 0.043	3.759GlyLys: 3.759 ± 0.054	8.642GlyLeu: 8.642 ± 0.082	2.331GlyMet: 2.331 ± 0.036	2.457GlyAsn: 2.457 ± 0.054	2.723GlyPro: 2.723 ± 0.043	3.483GlyGln: 3.483 ± 0.044	5.478GlyArg: 5.478 ± 0.057	4.607GlySer: 4.607 ± 0.072	3.845GlyThr: 3.845 ± 0.059	6.299GlyVal: 6.299 ± 0.062	1.282GlyTrp: 1.282 ± 0.026	2.439GlyTyr: 2.439 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	2.66HisAla: 2.66 ± 0.041	0.269HisCys: 0.269 ± 0.011	1.148HisAsp: 1.148 ± 0.027	1.016HisGlu: 1.016 ± 0.025	0.907HisPhe: 0.907 ± 0.025	2.131HisGly: 2.131 ± 0.04	0.609HisHis: 0.609 ± 0.021	0.976HisIle: 0.976 ± 0.022	0.633HisLys: 0.633 ± 0.021	2.146HisLeu: 2.146 ± 0.034	0.482HisMet: 0.482 ± 0.017	0.616HisAsn: 0.616 ± 0.018	1.266HisPro: 1.266 ± 0.027	0.817HisGln: 0.817 ± 0.022	1.236HisArg: 1.236 ± 0.027	1.16HisSer: 1.16 ± 0.024	1.029HisThr: 1.029 ± 0.023	1.335HisVal: 1.335 ± 0.024	0.354HisTrp: 0.354 ± 0.014	0.675HisTyr: 0.675 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.157IleAla: 6.157 ± 0.067	0.416IleCys: 0.416 ± 0.014	3.155IleAsp: 3.155 ± 0.038	2.939IleGlu: 2.939 ± 0.044	1.282IlePhe: 1.282 ± 0.023	4.169IleGly: 4.169 ± 0.051	0.854IleHis: 0.854 ± 0.021	1.703IleIle: 1.703 ± 0.035	1.688IleLys: 1.688 ± 0.031	3.485IleLeu: 3.485 ± 0.05	0.817IleMet: 0.817 ± 0.023	1.574IleAsn: 1.574 ± 0.03	1.818IlePro: 1.818 ± 0.029	1.162IleGln: 1.162 ± 0.027	2.341IleArg: 2.341 ± 0.033	2.589IleSer: 2.589 ± 0.04	2.169IleThr: 2.169 ± 0.038	3.563IleVal: 3.563 ± 0.047	0.429IleTrp: 0.429 ± 0.017	1.007IleTyr: 1.007 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.089LysAla: 4.089 ± 0.059	0.183LysCys: 0.183 ± 0.009	1.67LysAsp: 1.67 ± 0.035	1.626LysGlu: 1.626 ± 0.033	1.076LysPhe: 1.076 ± 0.025	2.343LysGly: 2.343 ± 0.038	0.726LysHis: 0.726 ± 0.021	1.743LysIle: 1.743 ± 0.033	1.514LysLys: 1.514 ± 0.04	4.118LysLeu: 4.118 ± 0.054	0.962LysMet: 0.962 ± 0.023	1.207LysAsn: 1.207 ± 0.03	2.07LysPro: 2.07 ± 0.044	1.515LysGln: 1.515 ± 0.029	2.218LysArg: 2.218 ± 0.038	1.908LysSer: 1.908 ± 0.032	1.901LysThr: 1.901 ± 0.037	2.53LysVal: 2.53 ± 0.041	0.386LysTrp: 0.386 ± 0.013	0.78LysTyr: 0.78 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	17.148LeuAla: 17.148 ± 0.139	1.073LeuCys: 1.073 ± 0.024	6.096LeuAsp: 6.096 ± 0.058	5.192LeuGlu: 5.192 ± 0.059	3.627LeuPhe: 3.627 ± 0.057	8.655LeuGly: 8.655 ± 0.07	2.295LeuHis: 2.295 ± 0.032	4.227LeuIle: 4.227 ± 0.05	3.645LeuLys: 3.645 ± 0.056	12.731LeuLeu: 12.731 ± 0.115	2.309LeuMet: 2.309 ± 0.037	3.192LeuAsn: 3.192 ± 0.048	6.122LeuPro: 6.122 ± 0.074	4.087LeuGln: 4.087 ± 0.044	8.136LeuArg: 8.136 ± 0.087	6.881LeuSer: 6.881 ± 0.091	5.184LeuThr: 5.184 ± 0.064	7.249LeuVal: 7.249 ± 0.07	1.179LeuTrp: 1.179 ± 0.031	2.265LeuTyr: 2.265 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	3.003MetAla: 3.003 ± 0.044	0.162MetCys: 0.162 ± 0.007	1.119MetAsp: 1.119 ± 0.029	1.028MetGlu: 1.028 ± 0.024	0.722MetPhe: 0.722 ± 0.021	1.66MetGly: 1.66 ± 0.032	0.509MetHis: 0.509 ± 0.016	0.96MetIle: 0.96 ± 0.019	0.96MetLys: 0.96 ± 0.023	2.823MetLeu: 2.823 ± 0.044	0.647MetMet: 0.647 ± 0.018	0.904MetAsn: 0.904 ± 0.021	1.345MetPro: 1.345 ± 0.03	1.045MetGln: 1.045 ± 0.027	1.683MetArg: 1.683 ± 0.032	1.603MetSer: 1.603 ± 0.029	1.372MetThr: 1.372 ± 0.027	1.643MetVal: 1.643 ± 0.032	0.211MetTrp: 0.211 ± 0.011	0.431MetTyr: 0.431 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.523AsnAla: 3.523 ± 0.056	0.275AsnCys: 0.275 ± 0.011	1.517AsnAsp: 1.517 ± 0.029	1.294AsnGlu: 1.294 ± 0.028	1.089AsnPhe: 1.089 ± 0.023	2.684AsnGly: 2.684 ± 0.046	0.611AsnHis: 0.611 ± 0.019	1.481AsnIle: 1.481 ± 0.028	1.013AsnLys: 1.013 ± 0.023	3.014AsnLeu: 3.014 ± 0.039	0.68AsnMet: 0.68 ± 0.017	1.058AsnAsn: 1.058 ± 0.033	1.77AsnPro: 1.77 ± 0.03	1.122AsnGln: 1.122 ± 0.027	1.681AsnArg: 1.681 ± 0.029	1.632AsnSer: 1.632 ± 0.039	1.567AsnThr: 1.567 ± 0.033	2.056AsnVal: 2.056 ± 0.038	0.449AsnTrp: 0.449 ± 0.016	0.892AsnTyr: 0.892 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	8.197ProAla: 8.197 ± 0.096	0.352ProCys: 0.352 ± 0.013	2.821ProAsp: 2.821 ± 0.045	2.737ProGlu: 2.737 ± 0.037	1.693ProPhe: 1.693 ± 0.03	4.16ProGly: 4.16 ± 0.046	0.981ProHis: 0.981 ± 0.022	1.712ProIle: 1.712 ± 0.034	1.409ProLys: 1.409 ± 0.033	5.347ProLeu: 5.347 ± 0.061	1.088ProMet: 1.088 ± 0.027	1.319ProAsn: 1.319 ± 0.033	2.714ProPro: 2.714 ± 0.052	2.106ProGln: 2.106 ± 0.037	2.761ProArg: 2.761 ± 0.043	2.591ProSer: 2.591 ± 0.034	2.267ProThr: 2.267 ± 0.04	3.521ProVal: 3.521 ± 0.049	0.606ProTrp: 0.606 ± 0.019	1.108ProTyr: 1.108 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	5.739GlnAla: 5.739 ± 0.064	0.301GlnCys: 0.301 ± 0.013	1.656GlnAsp: 1.656 ± 0.032	1.69GlnGlu: 1.69 ± 0.038	1.471GlnPhe: 1.471 ± 0.031	2.795GlnGly: 2.795 ± 0.041	1.04GlnHis: 1.04 ± 0.024	2.093GlnIle: 2.093 ± 0.069	1.149GlnLys: 1.149 ± 0.026	5.125GlnLeu: 5.125 ± 0.071	1.061GlnMet: 1.061 ± 0.024	1.017GlnAsn: 1.017 ± 0.023	2.059GlnPro: 2.059 ± 0.031	2.415GlnGln: 2.415 ± 0.043	3.377GlnArg: 3.377 ± 0.048	2.099GlnSer: 2.099 ± 0.033	1.947GlnThr: 1.947 ± 0.032	2.979GlnVal: 2.979 ± 0.042	0.562GlnTrp: 0.562 ± 0.019	0.903GlnTyr: 0.903 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.131ArgAla: 8.131 ± 0.085	0.559ArgCys: 0.559 ± 0.019	3.517ArgAsp: 3.517 ± 0.043	3.512ArgGlu: 3.512 ± 0.052	2.502ArgPhe: 2.502 ± 0.037	4.519ArgGly: 4.519 ± 0.051	1.855ArgHis: 1.855 ± 0.035	3.092ArgIle: 3.092 ± 0.044	2.018ArgLys: 2.018 ± 0.034	7.76ArgLeu: 7.76 ± 0.079	1.67ArgMet: 1.67 ± 0.026	1.828ArgAsn: 1.828 ± 0.032	3.062ArgPro: 3.062 ± 0.048	3.256ArgGln: 3.256 ± 0.049	5.025ArgArg: 5.025 ± 0.064	3.291ArgSer: 3.291 ± 0.045	2.577ArgThr: 2.577 ± 0.033	4.448ArgVal: 4.448 ± 0.052	0.939ArgTrp: 0.939 ± 0.025	1.915ArgTyr: 1.915 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	7.329SerAla: 7.329 ± 0.079	0.476SerCys: 0.476 ± 0.017	2.81SerAsp: 2.81 ± 0.046	2.463SerGlu: 2.463 ± 0.04	2.189SerPhe: 2.189 ± 0.032	5.549SerGly: 5.549 ± 0.082	1.2SerHis: 1.2 ± 0.028	2.549SerIle: 2.549 ± 0.041	1.932SerLys: 1.932 ± 0.032	5.735SerLeu: 5.735 ± 0.062	1.334SerMet: 1.334 ± 0.027	1.761SerAsn: 1.761 ± 0.039	2.435SerPro: 2.435 ± 0.037	1.93SerGln: 1.93 ± 0.036	3.209SerArg: 3.209 ± 0.043	3.436SerSer: 3.436 ± 0.061	2.95SerThr: 2.95 ± 0.062	4.003SerVal: 4.003 ± 0.051	0.758SerTrp: 0.758 ± 0.019	1.565SerTyr: 1.565 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.012ThrAla: 6.012 ± 0.085	0.333ThrCys: 0.333 ± 0.013	2.187ThrAsp: 2.187 ± 0.045	2.168ThrGlu: 2.168 ± 0.039	1.596ThrPhe: 1.596 ± 0.03	3.97ThrGly: 3.97 ± 0.055	0.863ThrHis: 0.863 ± 0.022	2.311ThrIle: 2.311 ± 0.036	1.293ThrLys: 1.293 ± 0.032	6.206ThrLeu: 6.206 ± 0.079	1.13ThrMet: 1.13 ± 0.025	1.247ThrAsn: 1.247 ± 0.03	3.199ThrPro: 3.199 ± 0.045	1.618ThrGln: 1.618 ± 0.032	2.737ThrArg: 2.737 ± 0.04	2.57ThrSer: 2.57 ± 0.047	2.68ThrThr: 2.68 ± 0.053	3.933ThrVal: 3.933 ± 0.05	0.531ThrTrp: 0.531 ± 0.018	1.047ThrTyr: 1.047 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	9.648ValAla: 9.648 ± 0.087	0.712ValCys: 0.712 ± 0.021	4.022ValAsp: 4.022 ± 0.052	4.001ValGlu: 4.001 ± 0.061	2.482ValPhe: 2.482 ± 0.041	5.55ValGly: 5.55 ± 0.058	1.321ValHis: 1.321 ± 0.025	2.91ValIle: 2.91 ± 0.041	2.554ValLys: 2.554 ± 0.046	7.84ValLeu: 7.84 ± 0.067	1.605ValMet: 1.605 ± 0.025	2.219ValAsn: 2.219 ± 0.035	3.277ValPro: 3.277 ± 0.037	2.451ValGln: 2.451 ± 0.035	4.453ValArg: 4.453 ± 0.049	4.05ValSer: 4.05 ± 0.044	3.424ValThr: 3.424 ± 0.051	5.649ValVal: 5.649 ± 0.061	0.835ValTrp: 0.835 ± 0.022	1.653ValTyr: 1.653 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.073TrpAla: 1.073 ± 0.024	0.141TrpCys: 0.141 ± 0.009	0.606TrpAsp: 0.606 ± 0.019	0.484TrpGlu: 0.484 ± 0.015	0.49TrpPhe: 0.49 ± 0.017	0.819TrpGly: 0.819 ± 0.021	0.332TrpHis: 0.332 ± 0.012	0.615TrpIle: 0.615 ± 0.018	0.437TrpLys: 0.437 ± 0.015	1.887TrpLeu: 1.887 ± 0.035	0.351TrpMet: 0.351 ± 0.014	0.448TrpAsn: 0.448 ± 0.016	0.588TrpPro: 0.588 ± 0.017	0.792TrpGln: 0.792 ± 0.021	1.208TrpArg: 1.208 ± 0.025	0.833TrpSer: 0.833 ± 0.022	0.58TrpThr: 0.58 ± 0.019	0.763TrpVal: 0.763 ± 0.02	0.219TrpTrp: 0.219 ± 0.011	0.292TrpTyr: 0.292 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.852TyrAla: 2.852 ± 0.042	0.276TyrCys: 0.276 ± 0.011	1.348TyrAsp: 1.348 ± 0.032	1.114TyrGlu: 1.114 ± 0.024	1.027TyrPhe: 1.027 ± 0.024	2.036TyrGly: 2.036 ± 0.041	0.54TyrHis: 0.54 ± 0.017	0.9TyrIle: 0.9 ± 0.022	0.783TyrLys: 0.783 ± 0.022	2.722TyrLeu: 2.722 ± 0.044	0.465TyrMet: 0.465 ± 0.016	0.734TyrAsn: 0.734 ± 0.02	1.175TyrPro: 1.175 ± 0.026	1.172TyrGln: 1.172 ± 0.026	1.813TyrArg: 1.813 ± 0.033	1.449TyrSer: 1.449 ± 0.03	1.247TyrThr: 1.247 ± 0.025	1.615TyrVal: 1.615 ± 0.026	0.371TyrTrp: 0.371 ± 0.014	0.73TyrTyr: 0.73 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5817 proteins (2007833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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