Vibrio phage VAP7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Vapseptimavirus; Vibrio virus VAP7

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TV63|A0A4Y5TV63_9CAUD Dihydrofolate reductase OS=Vibrio phage VAP7 OX=2584487 PE=4 SV=1
MM1 pKa = 7.64AATNFEE7 pKa = 4.58DD8 pKa = 4.43GKK10 pKa = 10.46HH11 pKa = 6.14LILMDD16 pKa = 3.78GTSLRR21 pKa = 11.84ISGQYY26 pKa = 10.56FDD28 pKa = 4.85IDD30 pKa = 3.85EE31 pKa = 5.06PNDD34 pKa = 3.56AVEE37 pKa = 4.09NAITEE42 pKa = 4.28FNEE45 pKa = 3.92ANGEE49 pKa = 4.27KK50 pKa = 10.43VLYY53 pKa = 10.45AYY55 pKa = 10.16DD56 pKa = 4.15AEE58 pKa = 4.04FDD60 pKa = 4.28YY61 pKa = 11.77YY62 pKa = 11.2FMAVSADD69 pKa = 3.91DD70 pKa = 4.31YY71 pKa = 11.61EE72 pKa = 4.6VATPEE77 pKa = 4.23FDD79 pKa = 3.6NLHH82 pKa = 6.09ARR84 pKa = 11.84LGAFLTLNHH93 pKa = 7.12IIFEE97 pKa = 4.35

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TXN5|A0A4Y5TXN5_9CAUD Baseplate wedge protein OS=Vibrio phage VAP7 OX=2584487 PE=4 SV=1
MM1 pKa = 7.77AKK3 pKa = 10.16GKK5 pKa = 8.42HH6 pKa = 4.36TNKK9 pKa = 9.75KK10 pKa = 10.1RR11 pKa = 11.84FVNEE15 pKa = 2.61NRR17 pKa = 11.84MAVRR21 pKa = 11.84WAGAQKK27 pKa = 10.32ILNFLSRR34 pKa = 11.84GTKK37 pKa = 9.31GRR39 pKa = 11.84VVAGDD44 pKa = 3.72PSAPQPLFRR53 pKa = 11.84LCEE56 pKa = 4.01MYY58 pKa = 11.04NLL60 pKa = 4.19

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

192

0

192

45000

29

1622

234.4

26.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.3 ± 0.179

1.131 ± 0.077

6.422 ± 0.126

7.002 ± 0.193

4.171 ± 0.105

6.48 ± 0.148

2.34 ± 0.103

6.464 ± 0.124

7.247 ± 0.164

7.824 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.153 ± 0.085

5.531 ± 0.159

3.651 ± 0.099

3.811 ± 0.108

4.749 ± 0.123

5.656 ± 0.134

5.953 ± 0.205

6.851 ± 0.138

1.407 ± 0.067

3.856 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski