Mytilus coruscus (Sea mussel)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64248 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J8B670|A0A6J8B670_MYTCO PLIN2 OS=Mytilus coruscus OX=42192 GN=MCOR_15481 PE=3 SV=1
MM1 pKa = 7.26 VCDD4 pKa = 4.11 LQVTTTLIRR13 pKa = 11.84 DD14 pKa = 3.85 FCDD17 pKa = 2.97 TDD19 pKa = 3.23 IDD21 pKa = 4.5 GDD23 pKa = 4.2 GVLNDD28 pKa = 3.92 EE29 pKa = 5.0 DD30 pKa = 3.91 NCVFISNPSQTDD42 pKa = 2.51 SDD44 pKa = 4.18 GNHH47 pKa = 6.72 RR48 pKa = 11.84 GDD50 pKa = 3.54 VCEE53 pKa = 4.77 SDD55 pKa = 3.48 YY56 pKa = 11.7 DD57 pKa = 3.66 QDD59 pKa = 3.92 GTIDD63 pKa = 3.76 TLDD66 pKa = 3.55 NCPNNKK72 pKa = 9.84 FINEE76 pKa = 4.23 SDD78 pKa = 3.15 FGTYY82 pKa = 9.14 TGLDD86 pKa = 3.93 LNPEE90 pKa = 4.26 LTLQPAPSWMILHH103 pKa = 6.89 GGKK106 pKa = 10.21 EE107 pKa = 3.8 IRR109 pKa = 11.84 QVSVTTKK116 pKa = 10.02 PVASVGNHH124 pKa = 6.16 RR125 pKa = 11.84 GDD127 pKa = 3.49 VCEE130 pKa = 4.77 SDD132 pKa = 3.48 YY133 pKa = 11.7 DD134 pKa = 3.66 QDD136 pKa = 3.92 GTIDD140 pKa = 3.76 TLDD143 pKa = 3.55 NCPNNKK149 pKa = 9.84 FINEE153 pKa = 3.93 SDD155 pKa = 3.25 FGYY158 pKa = 10.53 LYY160 pKa = 10.35 WFRR163 pKa = 11.84 PKK165 pKa = 10.6 SS166 pKa = 3.43
Molecular weight: 18.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.795
Rodwell 3.63
Grimsley 3.478
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.935
Patrickios 1.138
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A6J8EZ31|A0A6J8EZ31_MYTCO G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Mytilus coruscus OX=42192 GN=MCOR_57689 PE=3 SV=1
MM1 pKa = 6.96 YY2 pKa = 10.27 LAYY5 pKa = 10.19 NSNGIRR11 pKa = 11.84 TPVGGRR17 pKa = 11.84 VINKK21 pKa = 5.57 QTKK24 pKa = 9.2 HH25 pKa = 5.37 SKK27 pKa = 10.23 QSITHH32 pKa = 6.88 LSSQQSITHH41 pKa = 6.89 LSRR44 pKa = 11.84 VINNQTKK51 pKa = 9.85 HH52 pKa = 5.22 RR53 pKa = 11.84 QQSITHH59 pKa = 6.93 LSRR62 pKa = 11.84 VINKK66 pKa = 6.69 QTKK69 pKa = 9.13 HH70 pKa = 5.14 RR71 pKa = 11.84 QQSITHH77 pKa = 6.93 LSRR80 pKa = 11.84 VINKK84 pKa = 6.69 QTKK87 pKa = 9.13 HH88 pKa = 5.14 RR89 pKa = 11.84 QQSITHH95 pKa = 6.93 LSRR98 pKa = 11.84 VINKK102 pKa = 6.69 QTKK105 pKa = 9.13 HH106 pKa = 5.14 RR107 pKa = 11.84 QQSITHH113 pKa = 6.93 LSRR116 pKa = 11.84 VINKK120 pKa = 6.88 QTKK123 pKa = 8.56 HH124 pKa = 5.29 HH125 pKa = 5.48 QQSITHH131 pKa = 7.05 LSRR134 pKa = 11.84 VINKK138 pKa = 6.69 QTKK141 pKa = 9.13 HH142 pKa = 5.14 RR143 pKa = 11.84 QQSITHH149 pKa = 6.92 LSRR152 pKa = 11.84 VINNQTKK159 pKa = 10.15 HH160 pKa = 4.98 SQQSITHH167 pKa = 6.88 LSRR170 pKa = 11.84 VINKK174 pKa = 6.86 QTKK177 pKa = 9.52 HH178 pKa = 4.93 SQQSITHH185 pKa = 6.88 LSRR188 pKa = 11.84 VINKK192 pKa = 6.69 QTKK195 pKa = 9.13 HH196 pKa = 5.14 RR197 pKa = 11.84 QQSITHH203 pKa = 6.93 LSRR206 pKa = 11.84 VINKK210 pKa = 6.67 QTKK213 pKa = 9.2 HH214 pKa = 5.37 SKK216 pKa = 10.23 QSITHH221 pKa = 6.91 LSRR224 pKa = 11.84 IINKK228 pKa = 6.6 QTKK231 pKa = 9.12 HH232 pKa = 5.15 RR233 pKa = 11.84 QQSITHH239 pKa = 6.93 LSRR242 pKa = 11.84 VINKK246 pKa = 6.67 QTKK249 pKa = 9.2 HH250 pKa = 5.37 SKK252 pKa = 10.23 QSITHH257 pKa = 6.79 LSRR260 pKa = 11.84 VINKK264 pKa = 6.69 QTKK267 pKa = 9.13 HH268 pKa = 5.14 RR269 pKa = 11.84 QQSITHH275 pKa = 6.93 LSRR278 pKa = 11.84 VINKK282 pKa = 6.69 QTKK285 pKa = 9.13 HH286 pKa = 5.14 RR287 pKa = 11.84 QQSITHH293 pKa = 6.93 LSRR296 pKa = 11.84 VINKK300 pKa = 6.77 QTKK303 pKa = 8.19 HH304 pKa = 6.31 HH305 pKa = 6.24 II306 pKa = 3.92
Molecular weight: 36.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.503
IPC2_protein 11.389
IPC_protein 12.735
Toseland 12.91
ProMoST 13.393
Dawson 12.91
Bjellqvist 12.896
Wikipedia 13.378
Rodwell 12.778
Grimsley 12.939
Solomon 13.393
Lehninger 13.29
Nozaki 12.896
DTASelect 12.896
Thurlkill 12.896
EMBOSS 13.393
Sillero 12.896
Patrickios 12.486
IPC_peptide 13.393
IPC2_peptide 12.384
IPC2.peptide.svr19 9.108
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58248
6000
64248
29520201
66
9566
459.5
52.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.69 ± 0.008
2.365 ± 0.009
5.994 ± 0.007
6.508 ± 0.012
3.921 ± 0.006
5.099 ± 0.012
2.524 ± 0.005
6.532 ± 0.009
7.543 ± 0.013
8.393 ± 0.011
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.005
5.861 ± 0.009
4.184 ± 0.009
4.333 ± 0.008
4.757 ± 0.008
8.157 ± 0.012
6.38 ± 0.014
5.976 ± 0.008
1.044 ± 0.003
3.398 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here