Leptospira phage LE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Saintgironsvirus; Leptospira virus LE1

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6NE33|Q6NE33_9CAUD Uncharacterized protein OS=Leptospira phage LE1 OX=137511 GN=LE1-0010 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.49NKK4 pKa = 10.21NEE6 pKa = 4.24TKK8 pKa = 10.25EE9 pKa = 3.91QVAVEE14 pKa = 4.12EE15 pKa = 4.38TPEE18 pKa = 4.14TFTEE22 pKa = 4.43EE23 pKa = 3.92VMEE26 pKa = 4.92DD27 pKa = 3.6SEE29 pKa = 4.88SVQTAVVEE37 pKa = 4.57GVDD40 pKa = 3.76TVSGDD45 pKa = 3.54GTGEE49 pKa = 3.92PLPEE53 pKa = 4.42FDD55 pKa = 5.35QEE57 pKa = 4.31NPSDD61 pKa = 4.02LKK63 pKa = 11.17DD64 pKa = 3.74GNVLNPPPEE73 pKa = 4.26IEE75 pKa = 4.13EE76 pKa = 4.25EE77 pKa = 4.18NLGEE81 pKa = 3.97EE82 pKa = 5.04ASAEE86 pKa = 3.88DD87 pKa = 4.15LGIGFIQGEE96 pKa = 4.18IDD98 pKa = 3.42HH99 pKa = 6.99LSEE102 pKa = 4.65QEE104 pKa = 4.08KK105 pKa = 9.9TEE107 pKa = 4.08LGYY110 pKa = 9.44TISDD114 pKa = 3.37ILATPNQSQSIIVSMVINAILDD136 pKa = 3.61KK137 pKa = 11.08VGSVNPNAIEE147 pKa = 4.23ALGQLSTQIVEE158 pKa = 4.21RR159 pKa = 11.84AATQEE164 pKa = 3.83YY165 pKa = 7.88LTLVSEE171 pKa = 4.63FEE173 pKa = 4.55GVVSSGLPNATKK185 pKa = 10.67LSLYY189 pKa = 10.06SQQLDD194 pKa = 3.42QFAKK198 pKa = 10.7RR199 pKa = 11.84LGG201 pKa = 3.53

Molecular weight: 21.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6NE27|Q6NE27_9CAUD Uncharacterized protein OS=Leptospira phage LE1 OX=137511 GN=LE1-0016 PE=4 SV=1
MM1 pKa = 7.08FQGTTFGTRR10 pKa = 11.84LAEE13 pKa = 3.86IFKK16 pKa = 9.33TRR18 pKa = 11.84TRR20 pKa = 11.84IVSNKK25 pKa = 8.61FQQKK29 pKa = 9.11RR30 pKa = 11.84AKK32 pKa = 9.78QVIGKK37 pKa = 8.88EE38 pKa = 4.0RR39 pKa = 11.84VEE41 pKa = 4.35LLTFTHH47 pKa = 7.14RR48 pKa = 11.84FYY50 pKa = 11.28LHH52 pKa = 6.12YY53 pKa = 9.78MNPIHH58 pKa = 6.81RR59 pKa = 11.84WIVLALFKK67 pKa = 11.06LSVKK71 pKa = 10.67ANLRR75 pKa = 11.84KK76 pKa = 9.78KK77 pKa = 10.02PRR79 pKa = 11.84LSEE82 pKa = 3.81FLQFLSQFIQAFAGYY97 pKa = 9.86SVTHH101 pKa = 6.1VSNVPPKK108 pKa = 10.04IDD110 pKa = 3.41IYY112 pKa = 11.7SMGIRR117 pKa = 11.84IGLANFFWKK126 pKa = 10.26RR127 pKa = 11.84RR128 pKa = 11.84WLLMCKK134 pKa = 9.89IFLLGKK140 pKa = 10.28DD141 pKa = 3.56PDD143 pKa = 5.37FIDD146 pKa = 3.23SMKK149 pKa = 10.73RR150 pKa = 11.84LIVSNYY156 pKa = 9.93RR157 pKa = 11.84EE158 pKa = 4.27GLNLPEE164 pKa = 4.01PTANEE169 pKa = 4.1VNRR172 pKa = 11.84GSARR176 pKa = 11.84QVLPLKK182 pKa = 10.48QVV184 pKa = 2.88

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

22680

18

1971

280.0

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.098 ± 0.223

0.763 ± 0.102

5.692 ± 0.15

7.694 ± 0.262

4.815 ± 0.185

6.658 ± 0.339

1.42 ± 0.106

7.381 ± 0.23

8.884 ± 0.476

8.633 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.106

5.203 ± 0.165

4.043 ± 0.138

3.677 ± 0.153

4.709 ± 0.199

7.429 ± 0.236

5.088 ± 0.207

5.384 ± 0.209

1.235 ± 0.095

3.28 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski