Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Pyrenophora; Pyrenophora tritici-repentis

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2W907|B2W907_PYRTR TPR_REGION domain-containing protein OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) OX=426418 GN=PTRG_06465 PE=4 SV=1
MM1 pKa = 7.68HH2 pKa = 7.68DD3 pKa = 3.56AALVSHH9 pKa = 7.36DD10 pKa = 4.81PSPGPPVRR18 pKa = 11.84TFLYY22 pKa = 10.99NNDD25 pKa = 3.35APFYY29 pKa = 7.95PAPHH33 pKa = 7.29DD34 pKa = 4.27SLLDD38 pKa = 4.19DD39 pKa = 5.53DD40 pKa = 5.5PPPSYY45 pKa = 10.88EE46 pKa = 4.39DD47 pKa = 3.47TVGMIGAPPGYY58 pKa = 8.99GTFRR62 pKa = 11.84PYY64 pKa = 10.59TEE66 pKa = 4.52ASSIASSEE74 pKa = 4.27VEE76 pKa = 4.05SSDD79 pKa = 3.16RR80 pKa = 11.84ALPEE84 pKa = 3.78WVGQGLVVFIFTSIIYY100 pKa = 8.46GFWRR104 pKa = 11.84FINAPDD110 pKa = 4.39DD111 pKa = 4.0LSPIDD116 pKa = 4.41GWPGPPPPPP125 pKa = 4.36

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2WA31|B2WA31_PYRTR Uncharacterized protein OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) OX=426418 GN=PTRG_06839 PE=4 SV=1
MM1 pKa = 7.45WLKK4 pKa = 10.51PNRR7 pKa = 11.84IKK9 pKa = 10.93LINQIEE15 pKa = 4.25PRR17 pKa = 11.84HH18 pKa = 5.43SRR20 pKa = 11.84QNRR23 pKa = 11.84QNRR26 pKa = 11.84QNRR29 pKa = 11.84QNRR32 pKa = 11.84QNRR35 pKa = 11.84QNRR38 pKa = 11.84QNRR41 pKa = 11.84QNRR44 pKa = 11.84HH45 pKa = 4.01NRR47 pKa = 11.84QNRR50 pKa = 11.84QNRR53 pKa = 11.84QNRR56 pKa = 11.84QHH58 pKa = 6.36

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12062

0

12062

5414714

39

9693

448.9

49.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.753 ± 0.019

1.193 ± 0.009

5.727 ± 0.015

6.322 ± 0.025

3.615 ± 0.015

6.715 ± 0.025

2.427 ± 0.01

4.827 ± 0.017

5.235 ± 0.022

8.501 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.008

3.779 ± 0.013

6.104 ± 0.027

4.166 ± 0.017

5.97 ± 0.022

7.872 ± 0.029

6.198 ± 0.018

6.032 ± 0.016

1.432 ± 0.009

2.831 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski