Avian metapneumovirus (isolate Canada goose/Minnesota/15a/2001) (AMPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Metapneumovirus; Avian metapneumovirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q2Y2M6|NCAP_AMPV1 Nucleoprotein OS=Avian metapneumovirus (isolate Canada goose/Minnesota/15a/2001) OX=652954 GN=N PE=3 SV=1
MM1 pKa = 7.55SFPEE5 pKa = 4.62GKK7 pKa = 10.16DD8 pKa = 2.89ILLMGNEE15 pKa = 3.87AAKK18 pKa = 10.35AAEE21 pKa = 4.13AFQRR25 pKa = 11.84SLKK28 pKa = 10.57KK29 pKa = 9.59IGHH32 pKa = 6.76RR33 pKa = 11.84RR34 pKa = 11.84TQSIVGDD41 pKa = 3.83KK42 pKa = 10.32IITVSEE48 pKa = 4.34TVEE51 pKa = 4.09KK52 pKa = 9.04PTISKK57 pKa = 8.09STKK60 pKa = 8.33VTTPPEE66 pKa = 3.77RR67 pKa = 11.84RR68 pKa = 11.84NAWGEE73 pKa = 4.06KK74 pKa = 9.81PDD76 pKa = 3.46TTRR79 pKa = 11.84NQTEE83 pKa = 3.99EE84 pKa = 4.05ARR86 pKa = 11.84NEE88 pKa = 3.78ATLEE92 pKa = 4.11DD93 pKa = 3.77TSRR96 pKa = 11.84LYY98 pKa = 11.16EE99 pKa = 4.07EE100 pKa = 5.18VFAPTSDD107 pKa = 2.77GKK109 pKa = 9.05TPAEE113 pKa = 4.2EE114 pKa = 4.68GMEE117 pKa = 4.18TPEE120 pKa = 4.11KK121 pKa = 9.79PKK123 pKa = 10.94KK124 pKa = 10.23KK125 pKa = 9.2VTFKK129 pKa = 10.6NDD131 pKa = 2.56EE132 pKa = 4.08SGRR135 pKa = 11.84YY136 pKa = 6.94TKK138 pKa = 11.07LEE140 pKa = 3.96MEE142 pKa = 4.41ALEE145 pKa = 4.47LLSDD149 pKa = 4.56NEE151 pKa = 4.45DD152 pKa = 3.76DD153 pKa = 4.41DD154 pKa = 6.33AEE156 pKa = 4.67SSVLTFEE163 pKa = 5.05EE164 pKa = 4.99KK165 pKa = 9.88DD166 pKa = 3.38TSALSLEE173 pKa = 4.39ARR175 pKa = 11.84LEE177 pKa = 4.44SIDD180 pKa = 4.38EE181 pKa = 4.26KK182 pKa = 11.33LSMILGLLRR191 pKa = 11.84TLNVATAGPTAARR204 pKa = 11.84DD205 pKa = 4.19GIRR208 pKa = 11.84DD209 pKa = 3.41AMVGLRR215 pKa = 11.84EE216 pKa = 4.09EE217 pKa = 5.15LIADD221 pKa = 4.31IIKK224 pKa = 9.71EE225 pKa = 4.32AKK227 pKa = 9.86GKK229 pKa = 9.31AAEE232 pKa = 4.11MMKK235 pKa = 10.69EE236 pKa = 3.78EE237 pKa = 4.8AKK239 pKa = 10.48QKK241 pKa = 10.9SKK243 pKa = 10.6IGNGSVGLTEE253 pKa = 4.85KK254 pKa = 10.84AKK256 pKa = 9.9EE257 pKa = 3.87LNKK260 pKa = 10.09IVEE263 pKa = 4.67DD264 pKa = 4.06EE265 pKa = 4.41STSGEE270 pKa = 4.17SEE272 pKa = 4.1EE273 pKa = 4.4EE274 pKa = 3.98EE275 pKa = 4.11EE276 pKa = 5.02EE277 pKa = 4.08EE278 pKa = 5.79DD279 pKa = 4.42EE280 pKa = 4.99EE281 pKa = 5.09EE282 pKa = 4.98SNPDD286 pKa = 3.34DD287 pKa = 4.88DD288 pKa = 6.29LYY290 pKa = 11.69SLTMM294 pKa = 4.41

Molecular weight: 32.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q2Y2M0|SH_AMPV1 Small hydrophobic protein OS=Avian metapneumovirus (isolate Canada goose/Minnesota/15a/2001) OX=652954 GN=SH PE=3 SV=1
MM1 pKa = 7.11EE2 pKa = 4.47VKK4 pKa = 10.42VEE6 pKa = 4.08NVGKK10 pKa = 9.88SQEE13 pKa = 4.18LKK15 pKa = 10.47VKK17 pKa = 9.99VKK19 pKa = 10.96NFIKK23 pKa = 10.62RR24 pKa = 11.84SDD26 pKa = 3.73CKK28 pKa = 10.75KK29 pKa = 10.78KK30 pKa = 10.74LFALILGLVSFEE42 pKa = 3.78LTMNIMLSVMYY53 pKa = 10.62VEE55 pKa = 5.09SNEE58 pKa = 3.96ALSLCRR64 pKa = 11.84IQGTPAPRR72 pKa = 11.84DD73 pKa = 3.29NKK75 pKa = 10.64TNTEE79 pKa = 4.0NTKK82 pKa = 10.62KK83 pKa = 9.2EE84 pKa = 4.5TTFHH88 pKa = 5.8TTTTTRR94 pKa = 11.84DD95 pKa = 3.34PEE97 pKa = 4.07VRR99 pKa = 11.84EE100 pKa = 4.23TKK102 pKa = 6.87TTKK105 pKa = 10.36PKK107 pKa = 9.29TNEE110 pKa = 3.85GATSPSRR117 pKa = 11.84NLTTKK122 pKa = 10.91GDD124 pKa = 3.14IHH126 pKa = 5.48QTTRR130 pKa = 11.84ATTEE134 pKa = 3.89AEE136 pKa = 4.05LEE138 pKa = 4.3KK139 pKa = 10.64QSKK142 pKa = 7.06QTIEE146 pKa = 4.46PDD148 pKa = 3.16TSTKK152 pKa = 10.32KK153 pKa = 8.82HH154 pKa = 5.28TPTRR158 pKa = 11.84PSSEE162 pKa = 4.3SPTTTQATAQLTTPTAPKK180 pKa = 10.6ASIAPKK186 pKa = 10.05NRR188 pKa = 11.84QATTKK193 pKa = 9.16KK194 pKa = 8.79TEE196 pKa = 4.02TGTTTTSRR204 pKa = 11.84AKK206 pKa = 9.28KK207 pKa = 8.3TNNPTEE213 pKa = 4.23TATTTLKK220 pKa = 10.3ATTEE224 pKa = 4.02TGKK227 pKa = 10.92GKK229 pKa = 10.31EE230 pKa = 4.45GPTQHH235 pKa = 6.76TIKK238 pKa = 10.2EE239 pKa = 4.32QPEE242 pKa = 4.17TTAGEE247 pKa = 4.39TTTPQSRR254 pKa = 11.84RR255 pKa = 11.84TTSRR259 pKa = 11.84PAPTTKK265 pKa = 10.09TEE267 pKa = 4.14EE268 pKa = 4.02EE269 pKa = 4.35AEE271 pKa = 4.35TTKK274 pKa = 10.42TRR276 pKa = 11.84TTKK279 pKa = 9.28STQTSTGPPGPTRR292 pKa = 11.84STPSKK297 pKa = 9.71TATEE301 pKa = 4.07NNKK304 pKa = 8.77RR305 pKa = 11.84TTTIKK310 pKa = 10.59RR311 pKa = 11.84PNTANTDD318 pKa = 2.94SRR320 pKa = 11.84QQTRR324 pKa = 11.84TTAEE328 pKa = 3.77QDD330 pKa = 3.16RR331 pKa = 11.84QIQTKK336 pKa = 10.31AKK338 pKa = 7.08PTTNGAHH345 pKa = 6.33AQTTTTPEE353 pKa = 4.22HH354 pKa = 5.87NTDD357 pKa = 3.23TTNSTKK363 pKa = 10.44EE364 pKa = 3.74SSKK367 pKa = 10.64EE368 pKa = 3.86DD369 pKa = 3.16KK370 pKa = 8.18TTRR373 pKa = 11.84DD374 pKa = 3.8PSSKK378 pKa = 9.72TPTDD382 pKa = 3.26QEE384 pKa = 4.34DD385 pKa = 3.6ASKK388 pKa = 9.6GTTAANPRR396 pKa = 11.84KK397 pKa = 8.46NTEE400 pKa = 4.0ANTRR404 pKa = 11.84TPPTTTPTRR413 pKa = 11.84HH414 pKa = 4.38TTEE417 pKa = 4.32SATSTTGDD425 pKa = 3.04KK426 pKa = 10.44TKK428 pKa = 11.14AKK430 pKa = 4.72TTRR433 pKa = 11.84WKK435 pKa = 9.48STADD439 pKa = 3.48RR440 pKa = 11.84QPIRR444 pKa = 11.84NSTTAEE450 pKa = 3.97TKK452 pKa = 9.3TAQSKK457 pKa = 10.08QPTPKK462 pKa = 10.03QLSNNTTPEE471 pKa = 3.71NTTPPNNKK479 pKa = 9.6SSSQTDD485 pKa = 3.3AAPTEE490 pKa = 4.38EE491 pKa = 3.95IEE493 pKa = 4.47IRR495 pKa = 11.84SSLWRR500 pKa = 11.84RR501 pKa = 11.84RR502 pKa = 11.84YY503 pKa = 9.84VYY505 pKa = 10.21GPCRR509 pKa = 11.84EE510 pKa = 4.13NVLEE514 pKa = 4.98HH515 pKa = 6.76PMNPCFKK522 pKa = 11.06DD523 pKa = 3.15NTTWIYY529 pKa = 11.09SDD531 pKa = 3.31NGRR534 pKa = 11.84NLPAGYY540 pKa = 10.08YY541 pKa = 10.0DD542 pKa = 3.74SKK544 pKa = 9.64TDD546 pKa = 4.51KK547 pKa = 10.33IICYY551 pKa = 10.15GIYY554 pKa = 9.98RR555 pKa = 11.84GNSYY559 pKa = 10.82CYY561 pKa = 10.74GRR563 pKa = 11.84IEE565 pKa = 4.38CTCKK569 pKa = 10.71NGTGLLSYY577 pKa = 10.6CCNSYY582 pKa = 10.32NWSS585 pKa = 3.4

Molecular weight: 64.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4499

71

2005

499.9

55.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.024 ± 1.034

1.845 ± 0.281

4.445 ± 0.395

7.002 ± 0.967

3.134 ± 0.725

5.446 ± 0.436

1.467 ± 0.252

5.735 ± 0.521

7.691 ± 0.424

9.535 ± 1.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.334

5.335 ± 0.363

4.09 ± 0.556

3.401 ± 0.261

5.134 ± 0.434

8.18 ± 0.377

8.402 ± 2.618

6.668 ± 1.061

1.134 ± 0.251

2.845 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski