Microcoleus sp. PCC 7113

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Microcoleus; unclassified Microcoleus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6397 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9WLA7|K9WLA7_9CYAN PHB domain-containing protein OS=Microcoleus sp. PCC 7113 OX=1173027 GN=Mic7113_5329 PE=4 SV=1
MM1 pKa = 7.15FLEE4 pKa = 4.79TSSEE8 pKa = 4.39TLGSLSSDD16 pKa = 3.47TLKK19 pKa = 10.94HH20 pKa = 6.49LNTAYY25 pKa = 10.79LGLSGSDD32 pKa = 3.38LSSLEE37 pKa = 5.61LSDD40 pKa = 4.36LWGGNIVQGSSLDD53 pKa = 3.92PLSVAASEE61 pKa = 4.54RR62 pKa = 11.84SLEE65 pKa = 3.98TTYY68 pKa = 10.65TIEE71 pKa = 4.1QFNDD75 pKa = 3.29SLIDD79 pKa = 3.65SQTSISGIQGTKK91 pKa = 9.77WNDD94 pKa = 3.2LNGNGVQDD102 pKa = 3.41TGEE105 pKa = 4.31AGLAGWTIYY114 pKa = 10.89LDD116 pKa = 3.56QNNNGTLDD124 pKa = 3.37TGEE127 pKa = 4.34TSTTTDD133 pKa = 2.79ANGNYY138 pKa = 10.12SFTGLAAGTYY148 pKa = 8.13TVAEE152 pKa = 4.48VMQSGWQQTSPGAVTNGSFEE172 pKa = 4.38TGSLTGWNTIGSTEE186 pKa = 4.17VQTASYY192 pKa = 10.57GVTPTQGTYY201 pKa = 9.28QAVLTNGSGSVTDD214 pKa = 3.8SALEE218 pKa = 4.19SFFGWSAGTLDD229 pKa = 4.87GMGNGNATEE238 pKa = 4.38GSAMKK243 pKa = 10.63LSTITVAAGSTLTFDD258 pKa = 3.64WNFLTNEE265 pKa = 4.33STPSSYY271 pKa = 11.49NDD273 pKa = 3.31FAFVSINGLTEE284 pKa = 4.1LADD287 pKa = 3.96TNSTFALSPTVFTEE301 pKa = 3.55QTGYY305 pKa = 11.28KK306 pKa = 9.39KK307 pKa = 10.8FSYY310 pKa = 9.27TFRR313 pKa = 11.84AAGTYY318 pKa = 9.41TIGLGVVDD326 pKa = 5.52AGDD329 pKa = 3.66KK330 pKa = 10.32TVDD333 pKa = 3.23SALVVDD339 pKa = 4.91NFSLTPNGVYY349 pKa = 9.48QVNLASGQTVNNINFGSKK367 pKa = 9.84GVSVLAEE374 pKa = 4.49PNDD377 pKa = 4.47TIPSAIDD384 pKa = 3.28SGLSSSNPGTFKK396 pKa = 11.15DD397 pKa = 3.8KK398 pKa = 11.25GAIGDD403 pKa = 4.05NPNVASDD410 pKa = 4.13LDD412 pKa = 3.67VDD414 pKa = 4.68FVKK417 pKa = 10.68FDD419 pKa = 4.15LKK421 pKa = 11.19AGDD424 pKa = 4.24KK425 pKa = 9.93ATIDD429 pKa = 3.36IDD431 pKa = 4.03TNPLVSSLDD440 pKa = 3.44SFVQVFDD447 pKa = 3.67SAGNLVAFNDD457 pKa = 3.38NGAASGEE464 pKa = 4.3TSSLDD469 pKa = 3.27SFLEE473 pKa = 4.53FTATTTGTYY482 pKa = 10.28YY483 pKa = 10.95LAVSGKK489 pKa = 10.48GNSSYY494 pKa = 11.26NPNVQGSGASGSTGGYY510 pKa = 8.16NLEE513 pKa = 3.99VSVIPKK519 pKa = 9.82FSSNFGYY526 pKa = 11.07GLVDD530 pKa = 3.66AAAAVASATGQDD542 pKa = 3.25SAFADD547 pKa = 3.45VAYY550 pKa = 10.59FGGQNDD556 pKa = 3.42WGVNAVKK563 pKa = 10.48APEE566 pKa = 3.64AWAQGFTGEE575 pKa = 4.56GIVVAVIDD583 pKa = 3.47TGVDD587 pKa = 3.48YY588 pKa = 11.14KK589 pKa = 11.33HH590 pKa = 7.29PDD592 pKa = 3.15LDD594 pKa = 3.9ANIWVNEE601 pKa = 3.92GEE603 pKa = 4.2IAGNGVDD610 pKa = 5.25DD611 pKa = 5.65DD612 pKa = 5.02GNGYY616 pKa = 9.63IDD618 pKa = 6.14DD619 pKa = 3.94VNGWDD624 pKa = 5.35FVAGDD629 pKa = 4.02NDD631 pKa = 3.52AMDD634 pKa = 3.93VYY636 pKa = 11.74GHH638 pKa = 5.85GTHH641 pKa = 6.28VAGTIAAEE649 pKa = 4.18KK650 pKa = 10.64NDD652 pKa = 3.57FGVTGIAYY660 pKa = 7.92NAKK663 pKa = 9.4IMPVKK668 pKa = 10.78VLDD671 pKa = 4.51DD672 pKa = 4.13NGSGSNVDD680 pKa = 3.18VAAGIKK686 pKa = 9.56YY687 pKa = 10.35AADD690 pKa = 3.36NGADD694 pKa = 3.92VINLSLGGGYY704 pKa = 10.11SSEE707 pKa = 3.78IKK709 pKa = 10.66DD710 pKa = 3.47AVEE713 pKa = 3.59YY714 pKa = 8.91ATAKK718 pKa = 9.7GAVVVMAAGNEE729 pKa = 4.43SGSQPGFPASFANQWGVAVGAVDD752 pKa = 4.97SNNKK756 pKa = 8.5MADD759 pKa = 3.64FSNKK763 pKa = 9.93AGSTEE768 pKa = 3.82LDD770 pKa = 3.55YY771 pKa = 11.63VVAPGVDD778 pKa = 3.84VYY780 pKa = 11.22STTPGNTYY788 pKa = 10.5QSFNGTSMATPHH800 pKa = 5.64VAGVAALILSANPNLTPAEE819 pKa = 4.22VEE821 pKa = 4.17SLLTKK826 pKa = 9.15TANPTAITVV835 pKa = 3.4

Molecular weight: 85.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9WEJ7|K9WEJ7_9CYAN NB-ARC domain-containing protein OS=Microcoleus sp. PCC 7113 OX=1173027 GN=Mic7113_2380 PE=4 SV=1
MM1 pKa = 7.54GLPTRR6 pKa = 11.84GEE8 pKa = 4.21ARR10 pKa = 11.84NLAYY14 pKa = 9.53MKK16 pKa = 10.95LMMIGWLGIQGTASALPVKK35 pKa = 10.02PLALPPSPPLLTAQSPASPPSSKK58 pKa = 10.33CPRR61 pKa = 11.84DD62 pKa = 3.65VEE64 pKa = 4.59TLTSLMLKK72 pKa = 10.17DD73 pKa = 3.88LPSYY77 pKa = 10.77ANRR80 pKa = 11.84VMQRR84 pKa = 11.84SRR86 pKa = 11.84RR87 pKa = 11.84SVRR90 pKa = 11.84TPSSSSSVLVAGRR103 pKa = 11.84PEE105 pKa = 4.22FEE107 pKa = 4.91PLTLGPGSYY116 pKa = 9.34TPPASAIDD124 pKa = 4.2LEE126 pKa = 4.76PPQQVFFTTLEE137 pKa = 3.87RR138 pKa = 11.84QYY140 pKa = 11.47LGGKK144 pKa = 8.99ASYY147 pKa = 8.27MQLYY151 pKa = 9.09HH152 pKa = 7.0WLFLTQTPQGWRR164 pKa = 11.84VATMFSRR171 pKa = 11.84IGSSSGRR178 pKa = 11.84PPTPPQEE185 pKa = 4.15SSKK188 pKa = 10.96GVVGQAVNIWLRR200 pKa = 11.84DD201 pKa = 3.6CRR203 pKa = 11.84AGAIRR208 pKa = 11.84QSRR211 pKa = 11.84PRR213 pKa = 11.84NQKK216 pKa = 9.68

Molecular weight: 23.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6397

0

6397

2155269

29

2656

336.9

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.262 ± 0.032

0.984 ± 0.011

4.817 ± 0.023

6.43 ± 0.039

3.776 ± 0.02

6.773 ± 0.041

1.857 ± 0.018

6.129 ± 0.026

4.476 ± 0.032

11.161 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.875 ± 0.014

4.016 ± 0.029

4.997 ± 0.03

5.642 ± 0.031

5.456 ± 0.028

6.611 ± 0.029

5.73 ± 0.03

6.666 ± 0.024

1.461 ± 0.015

2.88 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski