Ophiostoma mitovirus 3a
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O92614|O92614_9VIRU RNA-dependent RNA polymerase OS=Ophiostoma mitovirus 3a OX=198597 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.55 RR3 pKa = 11.84 LTLSQNKK10 pKa = 9.7 SNQLTNNDD18 pKa = 4.15 LSNVGYY24 pKa = 8.54 ITKK27 pKa = 10.08 QLFPHH32 pKa = 7.65 WIRR35 pKa = 11.84 LLVWSLQLSPAPYY48 pKa = 9.7 KK49 pKa = 10.84 KK50 pKa = 10.03 FGSRR54 pKa = 11.84 IAILWKK60 pKa = 10.51 ANGVSFTVQYY70 pKa = 10.92 LKK72 pKa = 10.4 EE73 pKa = 4.06 CTRR76 pKa = 11.84 IVQHH80 pKa = 6.14 FVSGHH85 pKa = 5.08 PVFVTDD91 pKa = 4.26 VMPIGLAGGLPTIIPGTLRR110 pKa = 11.84 TLLRR114 pKa = 11.84 SKK116 pKa = 10.73 DD117 pKa = 3.25 SSTIRR122 pKa = 11.84 GVLSTLAVYY131 pKa = 10.52 RR132 pKa = 11.84 IMKK135 pKa = 7.39 MPCVLKK141 pKa = 10.84 LEE143 pKa = 5.12 SITDD147 pKa = 3.81 PFKK150 pKa = 11.15 GISDD154 pKa = 4.18 TLPKK158 pKa = 10.33 SEE160 pKa = 5.29 IINGLASLGFEE171 pKa = 4.04 IPKK174 pKa = 10.28 GRR176 pKa = 11.84 SKK178 pKa = 11.0 HH179 pKa = 6.3 LLTLSNPIIYY189 pKa = 10.25 LLSAGPNHH197 pKa = 7.09 SISMMGIWKK206 pKa = 9.75 DD207 pKa = 2.94 IYY209 pKa = 10.61 AWYY212 pKa = 10.22 VSPLFPTLLSFIGRR226 pKa = 11.84 MNRR229 pKa = 11.84 GNVLIDD235 pKa = 3.54 LLRR238 pKa = 11.84 AEE240 pKa = 4.17 VSYY243 pKa = 10.82 WEE245 pKa = 4.09 ATGVKK250 pKa = 9.77 PSVSPLDD257 pKa = 3.73 LKK259 pKa = 10.98 LGKK262 pKa = 10.04 LAIKK266 pKa = 10.33 EE267 pKa = 3.98 EE268 pKa = 4.33 AAGKK272 pKa = 10.46 ARR274 pKa = 11.84 VFAMADD280 pKa = 3.54 SITQSVMAPLNSWVFSKK297 pKa = 11.15 LKK299 pKa = 10.03 DD300 pKa = 3.63 LPMDD304 pKa = 3.56 GTFNQQAPLNRR315 pKa = 11.84 LVQLYY320 pKa = 10.31 QDD322 pKa = 4.19 GLLHH326 pKa = 7.16 DD327 pKa = 4.3 VEE329 pKa = 5.37 FYY331 pKa = 11.06 SYY333 pKa = 10.88 DD334 pKa = 3.48 LSSATDD340 pKa = 3.86 RR341 pKa = 11.84 LPMAFQKK348 pKa = 10.73 QIISVLFGSKK358 pKa = 9.11 FAKK361 pKa = 10.24 DD362 pKa = 3.07 WATLLVGRR370 pKa = 11.84 DD371 pKa = 3.46 WYY373 pKa = 11.38 LKK375 pKa = 9.9 DD376 pKa = 2.87 IPYY379 pKa = 9.72 RR380 pKa = 11.84 YY381 pKa = 9.82 SVGQPMGALSSWAMLALSHH400 pKa = 6.46 HH401 pKa = 7.02 VIVQIAAMRR410 pKa = 11.84 VGKK413 pKa = 10.58 LPFTNYY419 pKa = 11.0 ALLGDD424 pKa = 5.37 DD425 pKa = 4.97 IVIADD430 pKa = 3.86 KK431 pKa = 11.12 AVATSYY437 pKa = 11.22 HH438 pKa = 6.3 MIMTQILGVEE448 pKa = 4.2 INLSKK453 pKa = 11.1 SLVSNNSFEE462 pKa = 4.16 FAKK465 pKa = 10.7 RR466 pKa = 11.84 LVTMDD471 pKa = 4.04 GEE473 pKa = 4.64 VSAVGAKK480 pKa = 10.0 NLLVALKK487 pKa = 9.84 SRR489 pKa = 11.84 WGISSVILDD498 pKa = 4.72 LYY500 pKa = 11.4 NKK502 pKa = 9.86 GLALSEE508 pKa = 3.65 QDD510 pKa = 2.93 LRR512 pKa = 11.84 QRR514 pKa = 11.84 FSSIPTVSKK523 pKa = 10.83 QFGVDD528 pKa = 3.04 KK529 pKa = 10.86 LLWLVLGPFGFIPSKK544 pKa = 10.89 DD545 pKa = 3.44 GLSAFMKK552 pKa = 10.44 LNRR555 pKa = 11.84 SLSLVDD561 pKa = 3.1 MHH563 pKa = 7.44 ILLSCVDD570 pKa = 3.73 EE571 pKa = 5.3 AKK573 pKa = 10.62 FDD575 pKa = 4.16 LDD577 pKa = 3.63 KK578 pKa = 10.87 KK579 pKa = 8.12 TWEE582 pKa = 4.02 ANIQEE587 pKa = 4.63 TVHH590 pKa = 5.49 TLLRR594 pKa = 11.84 FGMLSEE600 pKa = 4.0 PAGFEE605 pKa = 4.07 VFSDD609 pKa = 4.36 FTSSPLYY616 pKa = 10.77 SFIRR620 pKa = 11.84 GQFGNKK626 pKa = 9.32 LSALVQDD633 pKa = 3.8 KK634 pKa = 10.0 PVRR637 pKa = 11.84 RR638 pKa = 11.84 LIFDD642 pKa = 3.79 GPLLHH647 pKa = 7.11 FNFYY651 pKa = 10.21 TEE653 pKa = 4.87 GWCDD657 pKa = 3.24 GLMEE661 pKa = 5.21 HH662 pKa = 6.59 LTKK665 pKa = 10.65 KK666 pKa = 9.73 IQSDD670 pKa = 3.76 SQEE673 pKa = 4.28 TVSPSNPFKK682 pKa = 10.31 DD683 pKa = 3.82 DD684 pKa = 3.57 KK685 pKa = 11.51 VILPLRR691 pKa = 11.84 GNIKK695 pKa = 10.79 GIFFKK700 pKa = 10.93 HH701 pKa = 5.16 VLALMAEE708 pKa = 4.33 RR709 pKa = 11.84 DD710 pKa = 3.74 PATVMRR716 pKa = 11.84 WMM718 pKa = 4.56
Molecular weight: 80.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.824
IPC2_protein 8.785
IPC_protein 8.668
Toseland 9.502
ProMoST 9.209
Dawson 9.75
Bjellqvist 9.428
Wikipedia 9.897
Rodwell 10.116
Grimsley 9.809
Solomon 9.765
Lehninger 9.736
Nozaki 9.545
DTASelect 9.399
Thurlkill 9.589
EMBOSS 9.926
Sillero 9.677
Patrickios 5.245
IPC_peptide 9.765
IPC2_peptide 7.892
IPC2.peptide.svr19 7.903
Protein with the highest isoelectric point:
>tr|O92614|O92614_9VIRU RNA-dependent RNA polymerase OS=Ophiostoma mitovirus 3a OX=198597 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.55 RR3 pKa = 11.84 LTLSQNKK10 pKa = 9.7 SNQLTNNDD18 pKa = 4.15 LSNVGYY24 pKa = 8.54 ITKK27 pKa = 10.08 QLFPHH32 pKa = 7.65 WIRR35 pKa = 11.84 LLVWSLQLSPAPYY48 pKa = 9.7 KK49 pKa = 10.84 KK50 pKa = 10.03 FGSRR54 pKa = 11.84 IAILWKK60 pKa = 10.51 ANGVSFTVQYY70 pKa = 10.92 LKK72 pKa = 10.4 EE73 pKa = 4.06 CTRR76 pKa = 11.84 IVQHH80 pKa = 6.14 FVSGHH85 pKa = 5.08 PVFVTDD91 pKa = 4.26 VMPIGLAGGLPTIIPGTLRR110 pKa = 11.84 TLLRR114 pKa = 11.84 SKK116 pKa = 10.73 DD117 pKa = 3.25 SSTIRR122 pKa = 11.84 GVLSTLAVYY131 pKa = 10.52 RR132 pKa = 11.84 IMKK135 pKa = 7.39 MPCVLKK141 pKa = 10.84 LEE143 pKa = 5.12 SITDD147 pKa = 3.81 PFKK150 pKa = 11.15 GISDD154 pKa = 4.18 TLPKK158 pKa = 10.33 SEE160 pKa = 5.29 IINGLASLGFEE171 pKa = 4.04 IPKK174 pKa = 10.28 GRR176 pKa = 11.84 SKK178 pKa = 11.0 HH179 pKa = 6.3 LLTLSNPIIYY189 pKa = 10.25 LLSAGPNHH197 pKa = 7.09 SISMMGIWKK206 pKa = 9.75 DD207 pKa = 2.94 IYY209 pKa = 10.61 AWYY212 pKa = 10.22 VSPLFPTLLSFIGRR226 pKa = 11.84 MNRR229 pKa = 11.84 GNVLIDD235 pKa = 3.54 LLRR238 pKa = 11.84 AEE240 pKa = 4.17 VSYY243 pKa = 10.82 WEE245 pKa = 4.09 ATGVKK250 pKa = 9.77 PSVSPLDD257 pKa = 3.73 LKK259 pKa = 10.98 LGKK262 pKa = 10.04 LAIKK266 pKa = 10.33 EE267 pKa = 3.98 EE268 pKa = 4.33 AAGKK272 pKa = 10.46 ARR274 pKa = 11.84 VFAMADD280 pKa = 3.54 SITQSVMAPLNSWVFSKK297 pKa = 11.15 LKK299 pKa = 10.03 DD300 pKa = 3.63 LPMDD304 pKa = 3.56 GTFNQQAPLNRR315 pKa = 11.84 LVQLYY320 pKa = 10.31 QDD322 pKa = 4.19 GLLHH326 pKa = 7.16 DD327 pKa = 4.3 VEE329 pKa = 5.37 FYY331 pKa = 11.06 SYY333 pKa = 10.88 DD334 pKa = 3.48 LSSATDD340 pKa = 3.86 RR341 pKa = 11.84 LPMAFQKK348 pKa = 10.73 QIISVLFGSKK358 pKa = 9.11 FAKK361 pKa = 10.24 DD362 pKa = 3.07 WATLLVGRR370 pKa = 11.84 DD371 pKa = 3.46 WYY373 pKa = 11.38 LKK375 pKa = 9.9 DD376 pKa = 2.87 IPYY379 pKa = 9.72 RR380 pKa = 11.84 YY381 pKa = 9.82 SVGQPMGALSSWAMLALSHH400 pKa = 6.46 HH401 pKa = 7.02 VIVQIAAMRR410 pKa = 11.84 VGKK413 pKa = 10.58 LPFTNYY419 pKa = 11.0 ALLGDD424 pKa = 5.37 DD425 pKa = 4.97 IVIADD430 pKa = 3.86 KK431 pKa = 11.12 AVATSYY437 pKa = 11.22 HH438 pKa = 6.3 MIMTQILGVEE448 pKa = 4.2 INLSKK453 pKa = 11.1 SLVSNNSFEE462 pKa = 4.16 FAKK465 pKa = 10.7 RR466 pKa = 11.84 LVTMDD471 pKa = 4.04 GEE473 pKa = 4.64 VSAVGAKK480 pKa = 10.0 NLLVALKK487 pKa = 9.84 SRR489 pKa = 11.84 WGISSVILDD498 pKa = 4.72 LYY500 pKa = 11.4 NKK502 pKa = 9.86 GLALSEE508 pKa = 3.65 QDD510 pKa = 2.93 LRR512 pKa = 11.84 QRR514 pKa = 11.84 FSSIPTVSKK523 pKa = 10.83 QFGVDD528 pKa = 3.04 KK529 pKa = 10.86 LLWLVLGPFGFIPSKK544 pKa = 10.89 DD545 pKa = 3.44 GLSAFMKK552 pKa = 10.44 LNRR555 pKa = 11.84 SLSLVDD561 pKa = 3.1 MHH563 pKa = 7.44 ILLSCVDD570 pKa = 3.73 EE571 pKa = 5.3 AKK573 pKa = 10.62 FDD575 pKa = 4.16 LDD577 pKa = 3.63 KK578 pKa = 10.87 KK579 pKa = 8.12 TWEE582 pKa = 4.02 ANIQEE587 pKa = 4.63 TVHH590 pKa = 5.49 TLLRR594 pKa = 11.84 FGMLSEE600 pKa = 4.0 PAGFEE605 pKa = 4.07 VFSDD609 pKa = 4.36 FTSSPLYY616 pKa = 10.77 SFIRR620 pKa = 11.84 GQFGNKK626 pKa = 9.32 LSALVQDD633 pKa = 3.8 KK634 pKa = 10.0 PVRR637 pKa = 11.84 RR638 pKa = 11.84 LIFDD642 pKa = 3.79 GPLLHH647 pKa = 7.11 FNFYY651 pKa = 10.21 TEE653 pKa = 4.87 GWCDD657 pKa = 3.24 GLMEE661 pKa = 5.21 HH662 pKa = 6.59 LTKK665 pKa = 10.65 KK666 pKa = 9.73 IQSDD670 pKa = 3.76 SQEE673 pKa = 4.28 TVSPSNPFKK682 pKa = 10.31 DD683 pKa = 3.82 DD684 pKa = 3.57 KK685 pKa = 11.51 VILPLRR691 pKa = 11.84 GNIKK695 pKa = 10.79 GIFFKK700 pKa = 10.93 HH701 pKa = 5.16 VLALMAEE708 pKa = 4.33 RR709 pKa = 11.84 DD710 pKa = 3.74 PATVMRR716 pKa = 11.84 WMM718 pKa = 4.56
Molecular weight: 80.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.824
IPC2_protein 8.785
IPC_protein 8.668
Toseland 9.502
ProMoST 9.209
Dawson 9.75
Bjellqvist 9.428
Wikipedia 9.897
Rodwell 10.116
Grimsley 9.809
Solomon 9.765
Lehninger 9.736
Nozaki 9.545
DTASelect 9.399
Thurlkill 9.589
EMBOSS 9.926
Sillero 9.677
Patrickios 5.245
IPC_peptide 9.765
IPC2_peptide 7.892
IPC2.peptide.svr19 7.903
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
718
718
718
718.0
80.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.128 ± 0.0
0.557 ± 0.0
5.292 ± 0.0
3.064 ± 0.0
5.153 ± 0.0
6.407 ± 0.0
1.95 ± 0.0
6.407 ± 0.0
6.685 ± 0.0
13.37 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.343 ± 0.0
3.621 ± 0.0
4.875 ± 0.0
3.343 ± 0.0
4.178 ± 0.0
9.331 ± 0.0
4.596 ± 0.0
6.964 ± 0.0
2.089 ± 0.0
2.646 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here