Clostridium vincentii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0BI67|A0A2T0BI67_9CLOT Glucans biosynthesis protein C OS=Clostridium vincentii OX=52704 GN=mdoC PE=4 SV=1
MM1 pKa = 7.04YY2 pKa = 9.94FKK4 pKa = 11.11LINEE8 pKa = 4.04KK9 pKa = 9.67HH10 pKa = 5.86AKK12 pKa = 9.07IVACIAIVCALLFTVTILFIPKK34 pKa = 9.65NVSDD38 pKa = 4.55DD39 pKa = 3.87ASGTAAYY46 pKa = 10.75SDD48 pKa = 3.54TLFNEE53 pKa = 4.97DD54 pKa = 4.13EE55 pKa = 4.33VSDD58 pKa = 3.83IQISIDD64 pKa = 3.61DD65 pKa = 4.4EE66 pKa = 4.44SWASILEE73 pKa = 4.23SPLDD77 pKa = 3.57EE78 pKa = 4.82TYY80 pKa = 10.71YY81 pKa = 11.12SCDD84 pKa = 2.62ITINGEE90 pKa = 3.71TLYY93 pKa = 11.18NVGIRR98 pKa = 11.84PKK100 pKa = 10.79GNTSLTQVASSDD112 pKa = 3.58SEE114 pKa = 4.18RR115 pKa = 11.84YY116 pKa = 8.73SFKK119 pKa = 11.24VKK121 pKa = 10.18FDD123 pKa = 3.91EE124 pKa = 4.51YY125 pKa = 11.58VDD127 pKa = 3.64GQTYY131 pKa = 10.97NGLDD135 pKa = 3.52KK136 pKa = 11.55LNLNNTYY143 pKa = 11.1SDD145 pKa = 3.07ATYY148 pKa = 9.78LKK150 pKa = 10.22EE151 pKa = 4.16YY152 pKa = 10.29ISYY155 pKa = 11.22DD156 pKa = 3.25LFDD159 pKa = 4.54FMGVTTPEE167 pKa = 4.05TAFSNITINSEE178 pKa = 3.63NRR180 pKa = 11.84GVYY183 pKa = 10.02LAVEE187 pKa = 4.28GLEE190 pKa = 3.87EE191 pKa = 4.73SYY193 pKa = 11.21LSRR196 pKa = 11.84NYY198 pKa = 10.87GSDD201 pKa = 2.93SGNLYY206 pKa = 9.98KK207 pKa = 10.71AEE209 pKa = 4.11GTGTDD214 pKa = 4.23LVWNGDD220 pKa = 3.63TQSNYY225 pKa = 10.9SGLKK229 pKa = 9.98DD230 pKa = 3.73NSVKK234 pKa = 10.84DD235 pKa = 3.35ITDD238 pKa = 3.2EE239 pKa = 4.17AFQKK243 pKa = 10.47VIDD246 pKa = 4.6MISNLNEE253 pKa = 3.67GTNLEE258 pKa = 4.51DD259 pKa = 4.5YY260 pKa = 10.89IDD262 pKa = 3.95VEE264 pKa = 4.19ATLKK268 pKa = 10.67YY269 pKa = 10.05FAVSTALVNLDD280 pKa = 4.05SYY282 pKa = 11.51QCNLYY287 pKa = 9.85HH288 pKa = 6.96NYY290 pKa = 10.39YY291 pKa = 9.86IYY293 pKa = 10.88EE294 pKa = 4.11EE295 pKa = 5.31DD296 pKa = 4.18GVCTILPWDD305 pKa = 4.06LNLSFGAFSGGGGGNNARR323 pKa = 11.84NNNDD327 pKa = 3.08GDD329 pKa = 3.99NATTTTNTTRR339 pKa = 11.84GIINFPIDD347 pKa = 3.87EE348 pKa = 4.52PTSATLEE355 pKa = 4.18DD356 pKa = 4.35SPLLSKK362 pKa = 10.82LLEE365 pKa = 4.0VDD367 pKa = 3.87EE368 pKa = 4.64YY369 pKa = 11.58KK370 pKa = 11.2DD371 pKa = 3.46MYY373 pKa = 10.77HH374 pKa = 7.66DD375 pKa = 3.92YY376 pKa = 11.26LNTIVEE382 pKa = 4.72EE383 pKa = 4.44YY384 pKa = 10.17FDD386 pKa = 4.2SGTFEE391 pKa = 4.76SKK393 pKa = 11.19VNTTSTLIDD402 pKa = 3.51SYY404 pKa = 11.88VEE406 pKa = 3.96NNPTAFYY413 pKa = 10.25GYY415 pKa = 9.88DD416 pKa = 3.28QFSASIDD423 pKa = 3.6EE424 pKa = 4.65LSTFGTYY431 pKa = 8.82RR432 pKa = 11.84AKK434 pKa = 10.59SIAMQLSGEE443 pKa = 4.17IPSTKK448 pKa = 10.1AGQSEE453 pKa = 4.55VDD455 pKa = 3.2LTTYY459 pKa = 10.61FSEE462 pKa = 5.61DD463 pKa = 3.61SVDD466 pKa = 3.88MNILGSMGGGNKK478 pKa = 10.47GGDD481 pKa = 3.26QGNNTDD487 pKa = 3.56TGTVAEE493 pKa = 4.54GQMPAGFPSGDD504 pKa = 3.55MDD506 pKa = 4.0QGGANMTPSSDD517 pKa = 3.46VQQNGSPNTDD527 pKa = 3.01TTKK530 pKa = 10.58SEE532 pKa = 4.09RR533 pKa = 11.84DD534 pKa = 3.72DD535 pKa = 5.12NGMQAPDD542 pKa = 4.26GNNMTRR548 pKa = 11.84PDD550 pKa = 3.85GMSKK554 pKa = 11.12GDD556 pKa = 3.69TNTSISITSIIIFATSFISIIIALVFSIKK585 pKa = 10.35FKK587 pKa = 10.62RR588 pKa = 11.84KK589 pKa = 9.39KK590 pKa = 10.44FKK592 pKa = 10.85LL593 pKa = 3.56

Molecular weight: 65.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0BBW7|A0A2T0BBW7_9CLOT Putative membrane protein insertion efficiency factor OS=Clostridium vincentii OX=52704 GN=CLVI_25140 PE=3 SV=1
MM1 pKa = 7.56FMTFQPKK8 pKa = 8.99KK9 pKa = 7.62RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.25RR14 pKa = 11.84EE15 pKa = 3.66HH16 pKa = 6.22GFRR19 pKa = 11.84KK20 pKa = 10.06RR21 pKa = 11.84MATPAGRR28 pKa = 11.84NILRR32 pKa = 11.84MRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.57GRR39 pKa = 11.84KK40 pKa = 8.93KK41 pKa = 9.66LTAA44 pKa = 4.2

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3379

0

3379

1015978

29

2723

300.7

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.654 ± 0.036

1.185 ± 0.014

5.606 ± 0.03

7.532 ± 0.045

4.366 ± 0.033

6.576 ± 0.038

1.323 ± 0.016

10.175 ± 0.05

8.831 ± 0.033

9.118 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.02

6.036 ± 0.04

2.705 ± 0.024

2.383 ± 0.023

3.327 ± 0.026

6.39 ± 0.034

5.034 ± 0.031

6.437 ± 0.029

0.674 ± 0.014

3.987 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski