Termite associated circular virus 1
Average proteome isoelectric point is 7.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1E327|A0A2P1E327_9VIRU Putative capsid protein OS=Termite associated circular virus 1 OX=2108549 PE=4 SV=1
MM1 pKa = 7.4 SFRR4 pKa = 11.84 WQGRR8 pKa = 11.84 YY9 pKa = 9.71 LFLTYY14 pKa = 10.59 AQAGNDD20 pKa = 3.57 FTPNEE25 pKa = 4.85 LITFIRR31 pKa = 11.84 EE32 pKa = 4.06 SGRR35 pKa = 11.84 KK36 pKa = 6.95 QPGYY40 pKa = 10.32 ILIGKK45 pKa = 7.84 EE46 pKa = 3.66 RR47 pKa = 11.84 HH48 pKa = 5.11 QDD50 pKa = 3.17 GGLHH54 pKa = 4.6 YY55 pKa = 10.43 HH56 pKa = 7.07 CFVDD60 pKa = 3.52 WVKK63 pKa = 10.85 RR64 pKa = 11.84 FSFTNAARR72 pKa = 11.84 FDD74 pKa = 4.26 FGGHH78 pKa = 6.44 HH79 pKa = 6.9 PNISPVNSPKK89 pKa = 10.81 ACLDD93 pKa = 3.6 YY94 pKa = 10.92 CSKK97 pKa = 11.29 DD98 pKa = 2.92 GDD100 pKa = 4.05 TTSWGTPPDD109 pKa = 3.73 LDD111 pKa = 4.07 SNHH114 pKa = 6.47 RR115 pKa = 11.84 EE116 pKa = 4.0 SRR118 pKa = 11.84 NEE120 pKa = 3.38 LWSRR124 pKa = 11.84 LLDD127 pKa = 3.67 TATNPPEE134 pKa = 3.81 FLQLVRR140 pKa = 11.84 EE141 pKa = 4.11 HH142 pKa = 6.84 APYY145 pKa = 11.07 DD146 pKa = 3.2 FATRR150 pKa = 11.84 YY151 pKa = 6.46 TQLDD155 pKa = 3.43 TMARR159 pKa = 11.84 AVYY162 pKa = 10.03 RR163 pKa = 11.84 PRR165 pKa = 11.84 TNFTSEE171 pKa = 4.23 FEE173 pKa = 4.75 HH174 pKa = 7.41 EE175 pKa = 5.29 DD176 pKa = 3.19 FTLPEE181 pKa = 5.12 GIEE184 pKa = 4.17 GWISSLFTKK193 pKa = 9.87 TRR195 pKa = 11.84 TAPYY199 pKa = 9.66 DD200 pKa = 3.73 PSHH203 pKa = 6.9 SSSSATLVPEE213 pKa = 4.18 RR214 pKa = 11.84 PVGRR218 pKa = 11.84 GRR220 pKa = 11.84 LGTTYY225 pKa = 10.46 TSRR228 pKa = 11.84 DD229 pKa = 3.31 LSTSTNSMPRR239 pKa = 11.84 QTTSFSTTAKK249 pKa = 9.62 SSSYY253 pKa = 8.22 QTTSPGWGAGASSKK267 pKa = 10.2 QQTSTEE273 pKa = 4.31 EE274 pKa = 4.02 KK275 pKa = 9.54 EE276 pKa = 4.27 HH277 pKa = 6.34
Molecular weight: 31.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.442
IPC2_protein 6.338
IPC_protein 6.532
Toseland 6.825
ProMoST 6.693
Dawson 6.898
Bjellqvist 6.795
Wikipedia 6.912
Rodwell 6.898
Grimsley 6.985
Solomon 6.912
Lehninger 6.912
Nozaki 7.117
DTASelect 7.307
Thurlkill 7.337
EMBOSS 7.337
Sillero 7.278
Patrickios 4.253
IPC_peptide 6.927
IPC2_peptide 7.088
IPC2.peptide.svr19 6.979
Protein with the highest isoelectric point:
>tr|A0A2P1E327|A0A2P1E327_9VIRU Putative capsid protein OS=Termite associated circular virus 1 OX=2108549 PE=4 SV=1
MM1 pKa = 7.86 PYY3 pKa = 10.3 SKK5 pKa = 10.16 RR6 pKa = 11.84 RR7 pKa = 11.84 YY8 pKa = 6.51 TRR10 pKa = 11.84 KK11 pKa = 9.11 PYY13 pKa = 10.11 RR14 pKa = 11.84 RR15 pKa = 11.84 STRR18 pKa = 11.84 KK19 pKa = 5.8 TTTRR23 pKa = 11.84 RR24 pKa = 11.84 TNRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 SGLTKK34 pKa = 9.26 TVARR38 pKa = 11.84 ISRR41 pKa = 11.84 SVQLRR46 pKa = 11.84 QVEE49 pKa = 4.62 TKK51 pKa = 10.73 RR52 pKa = 11.84 NITINLVAAPTPSTNANWQYY72 pKa = 10.2 YY73 pKa = 7.34 YY74 pKa = 11.23 RR75 pKa = 11.84 NVFVDD80 pKa = 4.02 LANGVDD86 pKa = 3.66 SDD88 pKa = 5.33 DD89 pKa = 4.27 IIGNEE94 pKa = 4.04 FVDD97 pKa = 4.6 PYY99 pKa = 11.09 VTFKK103 pKa = 10.87 WNVQFDD109 pKa = 4.14 INNLRR114 pKa = 11.84 SLNTSNTGVQTVKK127 pKa = 8.15 WWMMLVACNDD137 pKa = 3.67 YY138 pKa = 11.26 LPATVSGFNSIVPGTSTDD156 pKa = 3.41 PGGWWVQPDD165 pKa = 3.9 PTKK168 pKa = 9.19 MTFNGNNVKK177 pKa = 9.84 ILKK180 pKa = 10.4 KK181 pKa = 7.79 MTRR184 pKa = 11.84 QWHH187 pKa = 5.27 LTSIPSVVGTATVSGTEE204 pKa = 3.93 QFSGKK209 pKa = 8.66 MKK211 pKa = 9.93 YY212 pKa = 8.43 RR213 pKa = 11.84 WRR215 pKa = 11.84 RR216 pKa = 11.84 GKK218 pKa = 8.81 KK219 pKa = 7.56 TFEE222 pKa = 4.1 DD223 pKa = 4.0 RR224 pKa = 11.84 NTIEE228 pKa = 3.73 EE229 pKa = 4.23 DD230 pKa = 2.99 KK231 pKa = 11.16 TRR233 pKa = 11.84 VSQLRR238 pKa = 11.84 GMNYY242 pKa = 10.38 YY243 pKa = 9.76 IVTGWWVPTALSGTPLAAPIFYY265 pKa = 10.76 SVDD268 pKa = 2.82 QYY270 pKa = 11.89 LYY272 pKa = 10.86 FKK274 pKa = 10.98 DD275 pKa = 3.63 PP276 pKa = 3.56
Molecular weight: 31.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.794
IPC_protein 10.321
Toseland 10.365
ProMoST 10.072
Dawson 10.54
Bjellqvist 10.233
Wikipedia 10.745
Rodwell 10.877
Grimsley 10.613
Solomon 10.584
Lehninger 10.54
Nozaki 10.335
DTASelect 10.233
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.496
IPC_peptide 10.584
IPC2_peptide 9.004
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
553
276
277
276.5
31.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.702 ± 0.532
0.723 ± 0.268
5.063 ± 0.262
3.978 ± 1.34
4.702 ± 0.801
6.329 ± 0.395
1.989 ± 1.208
3.436 ± 0.677
4.702 ± 1.083
5.787 ± 0.53
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.808 ± 0.541
5.425 ± 1.353
5.967 ± 0.396
3.617 ± 0.005
8.137 ± 0.415
9.042 ± 1.602
11.573 ± 0.016
5.606 ± 2.295
2.893 ± 0.542
4.521 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here