Spirochaetia bacterium

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; unclassified Spirochaetia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2X9L9|A0A4Q2X9L9_9SPIR GMC_OxRdtase_N domain-containing protein (Fragment) OS=Spirochaetia bacterium OX=2053615 GN=EOP69_01735 PE=3 SV=1
MM1 pKa = 7.47TKK3 pKa = 10.45FLGLGAAAIAGLALAGCSVTGGDD26 pKa = 5.05LIRR29 pKa = 11.84PGEE32 pKa = 4.05PTATLIIDD40 pKa = 3.92NATGGYY46 pKa = 9.71LDD48 pKa = 4.49AVVISDD54 pKa = 4.82CDD56 pKa = 3.31NFTYY60 pKa = 10.53GFNRR64 pKa = 11.84LSDD67 pKa = 3.96GDD69 pKa = 4.56SIPPGGQRR77 pKa = 11.84QFTLSAGCWDD87 pKa = 3.5VGGGEE92 pKa = 4.19FAGGEE97 pKa = 4.01AYY99 pKa = 9.97EE100 pKa = 4.58RR101 pKa = 11.84MVLTPGMVMTYY112 pKa = 10.37SITDD116 pKa = 3.2

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2XCF2|A0A4Q2XCF2_9SPIR S9 family peptidase (Fragment) OS=Spirochaetia bacterium OX=2053615 GN=EOP69_01235 PE=4 SV=1
MM1 pKa = 6.8VRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84LSQTFTPSATMKK17 pKa = 9.7IQRR20 pKa = 11.84LAMCVASVLMSGALGLTGCATRR42 pKa = 11.84TTADD46 pKa = 2.97TWPTRR51 pKa = 11.84VVAADD56 pKa = 3.21QLRR59 pKa = 11.84PMEE62 pKa = 4.38RR63 pKa = 11.84LRR65 pKa = 11.84LSMPRR70 pKa = 11.84RR71 pKa = 11.84NLSDD75 pKa = 3.35PMPNGTVVLRR85 pKa = 11.84VHH87 pKa = 6.61VDD89 pKa = 2.88EE90 pKa = 5.67HH91 pKa = 6.83GVVRR95 pKa = 11.84KK96 pKa = 7.65TALVDD101 pKa = 3.52SSGNAVLDD109 pKa = 3.96AAAQRR114 pKa = 11.84ALVNAKK120 pKa = 9.06FVPYY124 pKa = 10.06RR125 pKa = 11.84EE126 pKa = 4.39AGEE129 pKa = 4.39TVAVTTLMPVSVKK142 pKa = 10.64ASSQCQGLRR151 pKa = 11.84PLDD154 pKa = 3.76CC155 pKa = 5.3

Molecular weight: 16.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

325

0

325

69931

26

1229

215.2

23.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.195 ± 0.175

0.881 ± 0.042

5.643 ± 0.122

5.075 ± 0.152

3.709 ± 0.083

8.028 ± 0.138

2.018 ± 0.071

5.666 ± 0.118

3.472 ± 0.126

10.141 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.083

2.964 ± 0.097

4.952 ± 0.104

3.246 ± 0.071

6.701 ± 0.173

6.391 ± 0.118

5.859 ± 0.112

7.59 ± 0.121

1.403 ± 0.068

2.452 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski