Clostridium phage CPD4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5H2TK35|A0A5H2TK35_9CAUD Uncharacterized protein OS=Clostridium phage CPD4 OX=2483607 GN=CPD4_20 PE=4 SV=1
MM1 pKa = 7.34NNKK4 pKa = 9.03KK5 pKa = 9.95FQEE8 pKa = 4.07TLEE11 pKa = 3.99QLKK14 pKa = 10.68EE15 pKa = 3.82EE16 pKa = 4.68LEE18 pKa = 4.21IQGVEE23 pKa = 4.23FNDD26 pKa = 3.68EE27 pKa = 3.98NDD29 pKa = 4.07LIAAVEE35 pKa = 3.94YY36 pKa = 10.18IIEE39 pKa = 4.1YY40 pKa = 9.86DD41 pKa = 3.7YY42 pKa = 11.52HH43 pKa = 6.81PEE45 pKa = 3.92QYY47 pKa = 10.97VEE49 pKa = 4.74DD50 pKa = 4.14TLCNYY55 pKa = 10.46PEE57 pKa = 4.12MFKK60 pKa = 11.08DD61 pKa = 3.66LL62 pKa = 4.02

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5H2TK45|A0A5H2TK45_9CAUD Uncharacterized protein OS=Clostridium phage CPD4 OX=2483607 GN=CPD4_60 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 10.32DD3 pKa = 3.1NNLIPLRR10 pKa = 11.84DD11 pKa = 3.33IAKK14 pKa = 9.58EE15 pKa = 3.71VGINEE20 pKa = 3.95RR21 pKa = 11.84KK22 pKa = 9.47ARR24 pKa = 11.84KK25 pKa = 9.07LLRR28 pKa = 11.84SHH30 pKa = 6.45EE31 pKa = 4.16NKK33 pKa = 10.35ANHH36 pKa = 6.34NYY38 pKa = 10.04YY39 pKa = 10.39WMFTPRR45 pKa = 11.84EE46 pKa = 3.88RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 5.37IVSVLKK55 pKa = 10.64RR56 pKa = 11.84LQQ58 pKa = 3.19

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16272

26

1061

206.0

23.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.279 ± 0.326

1.291 ± 0.16

6.477 ± 0.278

8.112 ± 0.433

3.577 ± 0.186

6.428 ± 0.324

1.653 ± 0.129

7.602 ± 0.279

9.71 ± 0.452

8.585 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.138

5.771 ± 0.205

3.441 ± 0.145

3.362 ± 0.156

3.945 ± 0.211

5.623 ± 0.205

5.248 ± 0.306

5.992 ± 0.257

1.26 ± 0.115

4.314 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski