Luteitalea sp.
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5191 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A7L1M9|A0A6A7L1M9_9BACT Cell division protein FtsQ OS=Luteitalea sp. OX=2004800 GN=ftsQ PE=3 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 GEE4 pKa = 3.72 KK5 pKa = 9.83 RR6 pKa = 11.84 AAFAVTAGSIVALVSCLATDD26 pKa = 4.41 ASADD30 pKa = 3.83 TFNLGIPSGWTCAGNCGTAAAAGVVTLAPDD60 pKa = 3.89 GGTQYY65 pKa = 11.32 GWVSTALGQGDD76 pKa = 3.89 VALEE80 pKa = 4.14 GVGGIGNPTNGSVLRR95 pKa = 11.84 SSLFSADD102 pKa = 4.08 AGDD105 pKa = 4.77 ALDD108 pKa = 5.26 FYY110 pKa = 11.23 FNYY113 pKa = 8.45 VTSDD117 pKa = 3.04 GAEE120 pKa = 3.91 FTDD123 pKa = 4.32 YY124 pKa = 11.3 AWARR128 pKa = 11.84 LLDD131 pKa = 4.02 PALDD135 pKa = 3.78 EE136 pKa = 4.36 VALLFTARR144 pKa = 11.84 TAEE147 pKa = 4.12 SGSIVPGFAMPSPQATLSPASVPIISGGPAWSPLGADD184 pKa = 4.06 SGACYY189 pKa = 10.19 DD190 pKa = 4.25 AGCGYY195 pKa = 9.09 TGWVLSNFAIASAGNYY211 pKa = 8.82 ILEE214 pKa = 4.38 FGVTNWTDD222 pKa = 2.99 EE223 pKa = 4.65 FYY225 pKa = 11.06 DD226 pKa = 3.73 SGLAFDD232 pKa = 4.73 GVTVGGVPIDD242 pKa = 3.89 EE243 pKa = 5.11 PPPVQAPEE251 pKa = 4.09 PATLMLLGTGLVGLLGIRR269 pKa = 11.84 RR270 pKa = 11.84 RR271 pKa = 11.84 SQAA274 pKa = 2.96
Molecular weight: 27.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.884
Patrickios 0.528
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A6A7L0H1|A0A6A7L0H1_9BACT DUF1698 domain-containing protein OS=Luteitalea sp. OX=2004800 GN=GEU99_06815 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.03 QPSRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 LRR14 pKa = 11.84 THH16 pKa = 6.39 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.37 NGRR28 pKa = 11.84 LVVTRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.12 GRR39 pKa = 11.84 KK40 pKa = 8.24 RR41 pKa = 11.84 LTVSGTTRR49 pKa = 11.84 PGTVGPRR56 pKa = 11.84 RR57 pKa = 3.51
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.286
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.486
Grimsley 12.925
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.193
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5191
0
5191
1943161
26
2435
374.3
40.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.539 ± 0.047
0.778 ± 0.01
5.599 ± 0.023
5.675 ± 0.03
3.636 ± 0.022
8.412 ± 0.031
2.226 ± 0.016
4.14 ± 0.017
2.12 ± 0.02
10.341 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.887 ± 0.013
2.468 ± 0.031
5.573 ± 0.019
3.272 ± 0.017
8.781 ± 0.036
5.492 ± 0.024
5.796 ± 0.023
8.318 ± 0.026
1.526 ± 0.015
2.423 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here