Aedes albopictus densovirus (isolate Boublik/1994) (AalDNV)
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q90186|NS2_AADNV Non-structural protein NS2 OS=Aedes albopictus densovirus (isolate Boublik/1994) OX=648330 GN=NS PE=4 SV=1
SS1 pKa = 5.82 TEE3 pKa = 3.97 YY4 pKa = 10.3 IRR6 pKa = 11.84 GINEE10 pKa = 3.57 GRR12 pKa = 11.84 VSSMEE17 pKa = 4.32 SVCSEE22 pKa = 4.44 HH23 pKa = 7.28 SPCEE27 pKa = 3.89 HH28 pKa = 6.74 GNIEE32 pKa = 4.54 CEE34 pKa = 5.65 CIFCWEE40 pKa = 4.7 HH41 pKa = 7.42 DD42 pKa = 4.04 AQCKK46 pKa = 7.7 SRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 EE51 pKa = 3.75 LDD53 pKa = 2.87 LGEE56 pKa = 4.2 PTGSEE61 pKa = 2.89 RR62 pKa = 11.84 GMANNYY68 pKa = 6.48 EE69 pKa = 4.04 QSRR72 pKa = 11.84 NEE74 pKa = 4.2 DD75 pKa = 3.81 LYY77 pKa = 10.86 CTEE80 pKa = 4.7 TVPSSAALQANTITEE95 pKa = 3.79 RR96 pKa = 11.84 DD97 pKa = 3.27 IRR99 pKa = 11.84 EE100 pKa = 4.43 DD101 pKa = 3.51 FTDD104 pKa = 3.38 QTVDD108 pKa = 3.18 NIYY111 pKa = 9.83 PQLHH115 pKa = 5.76 SGSRR119 pKa = 11.84 ASEE122 pKa = 3.74 QLEE125 pKa = 4.25 FAFPTIGTRR134 pKa = 11.84 SWEE137 pKa = 3.56 ILIRR141 pKa = 11.84 QSYY144 pKa = 8.5 EE145 pKa = 3.71 HH146 pKa = 7.02 LKK148 pKa = 10.32 PDD150 pKa = 3.51 YY151 pKa = 10.6 KK152 pKa = 11.15 EE153 pKa = 4.87 EE154 pKa = 4.37 DD155 pKa = 3.77 FQSHH159 pKa = 4.78 IRR161 pKa = 11.84 RR162 pKa = 11.84 VRR164 pKa = 11.84 RR165 pKa = 11.84 QLFPEE170 pKa = 3.73 KK171 pKa = 10.38 TMDD174 pKa = 3.73 NNGSQASTTQMLRR187 pKa = 11.84 DD188 pKa = 4.48 DD189 pKa = 4.38 IEE191 pKa = 4.9 RR192 pKa = 11.84 CGIEE196 pKa = 4.53 SIADD200 pKa = 3.53 SASEE204 pKa = 4.14 DD205 pKa = 3.47 NGDD208 pKa = 4.27 GVDD211 pKa = 4.02 GTCISTVDD219 pKa = 3.26 IQGNCIVNAHH229 pKa = 6.2 GNEE232 pKa = 3.78 QATGSKK238 pKa = 7.63 TRR240 pKa = 11.84 KK241 pKa = 9.02 IRR243 pKa = 11.84 ATTTPEE249 pKa = 3.51 SSEE252 pKa = 4.31 SKK254 pKa = 9.74 KK255 pKa = 10.36 HH256 pKa = 6.07 KK257 pKa = 10.47 GSNNQQNIQEE267 pKa = 4.26 QSSTTAIADD276 pKa = 3.24 GHH278 pKa = 6.84 DD279 pKa = 3.61 IVDD282 pKa = 3.93 GEE284 pKa = 4.32 LDD286 pKa = 3.52 GRR288 pKa = 11.84 SEE290 pKa = 4.57 SNRR293 pKa = 11.84 EE294 pKa = 3.24 TAYY297 pKa = 9.69 YY298 pKa = 10.31 TFVLHH303 pKa = 7.26 KK304 pKa = 10.43 NNCKK308 pKa = 10.21 EE309 pKa = 3.66 DD310 pKa = 2.98 WRR312 pKa = 11.84 YY313 pKa = 9.85 IATTRR318 pKa = 11.84 AKK320 pKa = 9.79 QAPSFITFDD329 pKa = 3.72 HH330 pKa = 7.1 GDD332 pKa = 3.85 HH333 pKa = 5.75 IHH335 pKa = 6.83 ILFSSSNTGGNSTRR349 pKa = 11.84 VRR351 pKa = 11.84 TRR353 pKa = 11.84 ITKK356 pKa = 9.82 FLSATSAGNAEE367 pKa = 3.98 ATITFSKK374 pKa = 11.0 VKK376 pKa = 10.19 FLRR379 pKa = 11.84 NYY381 pKa = 9.03 ILYY384 pKa = 9.52 CIRR387 pKa = 11.84 YY388 pKa = 9.33 GIEE391 pKa = 3.76 TVNIYY396 pKa = 10.54 GNKK399 pKa = 9.09 IQQQLTEE406 pKa = 4.25 AMDD409 pKa = 3.61 TFKK412 pKa = 11.29 VLFEE416 pKa = 4.42 NRR418 pKa = 11.84 DD419 pKa = 3.73 PNDD422 pKa = 3.43 VILDD426 pKa = 4.19 AGCKK430 pKa = 9.61 LYY432 pKa = 10.86 HH433 pKa = 6.09 EE434 pKa = 4.93 EE435 pKa = 4.4 KK436 pKa = 10.6 KK437 pKa = 10.6 EE438 pKa = 3.89 YY439 pKa = 7.85 QQKK442 pKa = 10.17 RR443 pKa = 11.84 CGQRR447 pKa = 11.84 KK448 pKa = 6.52 QQNLTDD454 pKa = 4.96 IILDD458 pKa = 3.79 KK459 pKa = 10.92 IKK461 pKa = 10.44 EE462 pKa = 4.23 KK463 pKa = 10.78 KK464 pKa = 8.38 ITTAQQWEE472 pKa = 4.47 NIIEE476 pKa = 4.16 PEE478 pKa = 4.67 FKK480 pKa = 10.12 IQLMKK485 pKa = 10.94 EE486 pKa = 3.78 FGLNVDD492 pKa = 4.19 SYY494 pKa = 8.03 VQRR497 pKa = 11.84 IVRR500 pKa = 11.84 IEE502 pKa = 3.78 RR503 pKa = 11.84 TRR505 pKa = 11.84 IQQIIKK511 pKa = 10.43 SKK513 pKa = 8.69 TLTEE517 pKa = 3.93 IMVEE521 pKa = 3.97 ILNEE525 pKa = 3.96 EE526 pKa = 4.45 YY527 pKa = 10.31 IKK529 pKa = 11.16 NFTPGEE535 pKa = 4.33 DD536 pKa = 3.23 NSKK539 pKa = 10.05 LQNIIQWIEE548 pKa = 3.64 YY549 pKa = 8.05 MFKK552 pKa = 10.64 EE553 pKa = 3.86 NNINIIHH560 pKa = 6.27 FLAWNEE566 pKa = 3.68 IIKK569 pKa = 9.87 PKK571 pKa = 10.06 RR572 pKa = 11.84 YY573 pKa = 9.83 KK574 pKa = 10.38 KK575 pKa = 9.96 INGMVLEE582 pKa = 5.46 GITNAGKK589 pKa = 10.41 SLILDD594 pKa = 3.63 NLLAMVKK601 pKa = 10.05 PEE603 pKa = 4.45 EE604 pKa = 4.29 IPRR607 pKa = 11.84 EE608 pKa = 3.78 RR609 pKa = 11.84 DD610 pKa = 2.95 NSGFHH615 pKa = 7.45 LDD617 pKa = 3.66 QLPGAGSVLFEE628 pKa = 4.58 EE629 pKa = 5.22 PMITPVNVGTWKK641 pKa = 10.82 LLLEE645 pKa = 4.64 GKK647 pKa = 8.26 TIKK650 pKa = 10.01 TDD652 pKa = 3.32 VKK654 pKa = 11.12 NKK656 pKa = 10.07 DD657 pKa = 3.23 KK658 pKa = 11.17 EE659 pKa = 4.56 PIEE662 pKa = 4.25 RR663 pKa = 11.84 TPTWITTATPITNNVHH679 pKa = 5.68 MNEE682 pKa = 4.07 TSQILQRR689 pKa = 11.84 IKK691 pKa = 10.83 LYY693 pKa = 10.29 IFKK696 pKa = 10.62 KK697 pKa = 9.96 SIQHH701 pKa = 6.66 RR702 pKa = 11.84 EE703 pKa = 3.65 DD704 pKa = 3.54 KK705 pKa = 10.28 YY706 pKa = 9.68 TINAQIQNKK715 pKa = 9.68 LISRR719 pKa = 11.84 PPGLVEE725 pKa = 5.08 PIHH728 pKa = 5.41 MAIVFVKK735 pKa = 10.26 NFKK738 pKa = 10.27 EE739 pKa = 3.88 IYY741 pKa = 9.99 KK742 pKa = 10.57 LIEE745 pKa = 4.35 EE746 pKa = 4.69 EE747 pKa = 4.95 DD748 pKa = 3.58 NAHH751 pKa = 5.59 TVNEE755 pKa = 4.01 KK756 pKa = 10.55 AIRR759 pKa = 11.84 LSEE762 pKa = 4.04 EE763 pKa = 3.87 AKK765 pKa = 10.52 QEE767 pKa = 3.98 AEE769 pKa = 3.92 EE770 pKa = 4.24 WQTALQWNTMEE781 pKa = 4.73 EE782 pKa = 4.1 EE783 pKa = 4.06 QKK785 pKa = 11.02 EE786 pKa = 4.24 NEE788 pKa = 4.49 KK789 pKa = 8.88 QTEE792 pKa = 4.19 DD793 pKa = 3.2 QDD795 pKa = 3.99 KK796 pKa = 10.63 EE797 pKa = 4.53 SEE799 pKa = 4.53 KK800 pKa = 10.25 EE801 pKa = 3.94 TATQQ805 pKa = 3.17
Molecular weight: 92.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.327
IPC2_protein 5.347
IPC_protein 5.334
Toseland 5.41
ProMoST 5.512
Dawson 5.385
Bjellqvist 5.474
Wikipedia 5.27
Rodwell 5.334
Grimsley 5.397
Solomon 5.385
Lehninger 5.347
Nozaki 5.537
DTASelect 5.664
Thurlkill 5.461
EMBOSS 5.397
Sillero 5.626
Patrickios 4.253
IPC_peptide 5.397
IPC2_peptide 5.626
IPC2.peptide.svr19 5.588
Protein with the highest isoelectric point:
>sp|Q90187|CAPSD_AADNV Capsid protein VP1/VP2 OS=Aedes albopictus densovirus (isolate Boublik/1994) OX=648330 GN=VP PE=3 SV=2
SS1 pKa = 6.17 VSAFFVGSMTHH12 pKa = 5.5 SVNPGGEE19 pKa = 3.63 NWIWEE24 pKa = 4.36 SQLEE28 pKa = 4.31 VRR30 pKa = 11.84 EE31 pKa = 3.97 EE32 pKa = 3.99 WPTITNNQGTKK43 pKa = 9.63 IYY45 pKa = 9.99 IAPRR49 pKa = 11.84 QYY51 pKa = 11.42 LLQLRR56 pKa = 11.84 CKK58 pKa = 7.69 QTPSRR63 pKa = 11.84 SEE65 pKa = 3.46 ISEE68 pKa = 4.45 KK69 pKa = 10.28 ISQIKK74 pKa = 9.24 PLITSTHH81 pKa = 6.52 NYY83 pKa = 6.57 TAAPEE88 pKa = 4.11 PLNSLNSHH96 pKa = 6.1 FQLLEE101 pKa = 3.96 QEE103 pKa = 4.17 AGKK106 pKa = 10.39 YY107 pKa = 10.5 LYY109 pKa = 11.33 VNLTNISSQIIRR121 pKa = 11.84 KK122 pKa = 9.75 KK123 pKa = 10.2 IFNHH127 pKa = 5.81 ILDD130 pKa = 4.04 VFEE133 pKa = 6.28 DD134 pKa = 3.95 SYY136 pKa = 11.86 SPKK139 pKa = 10.25 KK140 pKa = 10.11 LWIITGHH147 pKa = 6.09 RR148 pKa = 11.84 QAQPKK153 pKa = 10.18 CYY155 pKa = 7.62 EE156 pKa = 4.3 TTSKK160 pKa = 11.15 DD161 pKa = 3.62 AVLKK165 pKa = 10.97 ALLTAQAKK173 pKa = 6.97 ITEE176 pKa = 4.21 MEE178 pKa = 4.15 LMGRR182 pKa = 11.84 ASPPWIYY189 pKa = 10.74 KK190 pKa = 8.82 ATVLLTHH197 pKa = 6.7 MAMNRR202 pKa = 11.84 QQAVRR207 pKa = 11.84 PEE209 pKa = 4.31 RR210 pKa = 11.84 YY211 pKa = 8.67 EE212 pKa = 3.62 QQPRR216 pKa = 11.84 QNQVNQKK223 pKa = 9.35 NIKK226 pKa = 9.51 AAIINKK232 pKa = 9.35 IYY234 pKa = 10.28 KK235 pKa = 9.57 NKK237 pKa = 10.09 AVPPPSPTDD246 pKa = 3.53 TISSTEE252 pKa = 3.49 SWMEE256 pKa = 3.58 EE257 pKa = 3.71 VNQIEE262 pKa = 4.56 KK263 pKa = 10.01 QHH265 pKa = 5.32 TTHH268 pKa = 6.99 SSSTKK273 pKa = 8.58 ITAKK277 pKa = 9.22 RR278 pKa = 11.84 TGDD281 pKa = 3.15 ISPQQGPSKK290 pKa = 10.34 RR291 pKa = 11.84 RR292 pKa = 11.84 ALSPLITEE300 pKa = 4.48 TTSTSSSHH308 pKa = 6.37 HH309 pKa = 4.39 QTQEE313 pKa = 3.36 EE314 pKa = 4.54 TAQEE318 pKa = 4.31 SEE320 pKa = 4.37 PEE322 pKa = 4.25 SPSFLVQQAQEE333 pKa = 4.0 MQKK336 pKa = 10.42 RR337 pKa = 11.84 LSLFPKK343 pKa = 10.22 LNFSGTTFSIASVTVSKK360 pKa = 10.83 QSISMEE366 pKa = 3.53 IKK368 pKa = 10.69 SNNNN372 pKa = 2.93
Molecular weight: 42.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.775
IPC2_protein 8.682
IPC_protein 8.58
Toseland 9.414
ProMoST 9.121
Dawson 9.663
Bjellqvist 9.341
Wikipedia 9.809
Rodwell 10.014
Grimsley 9.721
Solomon 9.692
Lehninger 9.663
Nozaki 9.458
DTASelect 9.311
Thurlkill 9.502
EMBOSS 9.838
Sillero 9.589
Patrickios 5.219
IPC_peptide 9.692
IPC2_peptide 7.819
IPC2.peptide.svr19 7.808
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1532
355
805
510.7
58.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.157 ± 0.186
1.11 ± 0.436
3.851 ± 0.698
8.812 ± 1.403
3.198 ± 0.391
4.634 ± 0.636
2.48 ± 0.087
8.094 ± 1.312
6.527 ± 1.022
5.94 ± 0.189
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.35 ± 0.652
6.984 ± 0.772
4.047 ± 0.599
6.658 ± 0.461
5.157 ± 0.277
7.115 ± 1.273
9.138 ± 1.346
3.982 ± 0.228
1.371 ± 0.072
3.394 ± 0.881
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here