Sparus aurata papillomavirus 1
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2RWA6|A0A1B2RWA6_9PAPI SORF3 OS=Sparus aurata papillomavirus 1 OX=1885928 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 FKK4 pKa = 10.84 QDD6 pKa = 3.24 LSVLVPSFDD15 pKa = 5.87 DD16 pKa = 6.49 DD17 pKa = 4.96 DD18 pKa = 6.6 DD19 pKa = 5.01 DD20 pKa = 4.86 WLLCDD25 pKa = 4.93 EE26 pKa = 5.14 EE27 pKa = 5.57 FDD29 pKa = 5.47 DD30 pKa = 6.23 VLDD33 pKa = 3.51 MWIEE37 pKa = 4.01 EE38 pKa = 4.06 EE39 pKa = 4.2 FEE41 pKa = 3.98 LVIEE45 pKa = 4.26 IVHH48 pKa = 5.33 YY49 pKa = 10.75 VYY51 pKa = 10.62 II52 pKa = 4.57
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.958
IPC2_protein 3.427
IPC_protein 3.376
Toseland 3.172
ProMoST 3.579
Dawson 3.389
Bjellqvist 3.567
Wikipedia 3.363
Rodwell 3.223
Grimsley 3.096
Solomon 3.35
Lehninger 3.3
Nozaki 3.528
DTASelect 3.732
Thurlkill 3.261
EMBOSS 3.376
Sillero 3.503
Patrickios 1.761
IPC_peptide 3.338
IPC2_peptide 3.465
IPC2.peptide.svr19 3.66
Protein with the highest isoelectric point:
>tr|A0A1B2RWA7|A0A1B2RWA7_9PAPI L2 OS=Sparus aurata papillomavirus 1 OX=1885928 PE=4 SV=1
MM1 pKa = 8.29 LMDD4 pKa = 5.38 SILWLTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 10.46 YY14 pKa = 9.11 ITTSSSLTMEE24 pKa = 4.23 TFIMIVLPLHH34 pKa = 5.9 PQSLPLKK41 pKa = 7.8 RR42 pKa = 11.84 AKK44 pKa = 9.82 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 LRR52 pKa = 11.84 KK53 pKa = 9.56 LKK55 pKa = 10.5 APKK58 pKa = 9.75 RR59 pKa = 11.84 AQKK62 pKa = 10.56 LL63 pKa = 3.52
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.261
IPC2_protein 10.672
IPC_protein 12.091
Toseland 12.266
ProMoST 12.749
Dawson 12.266
Bjellqvist 12.252
Wikipedia 12.735
Rodwell 12.047
Grimsley 12.31
Solomon 12.749
Lehninger 12.647
Nozaki 12.266
DTASelect 12.252
Thurlkill 12.266
EMBOSS 12.749
Sillero 12.266
Patrickios 11.769
IPC_peptide 12.749
IPC2_peptide 11.74
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
2
9
2538
52
565
282.0
32.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.846 ± 0.617
1.812 ± 0.32
5.319 ± 0.752
7.447 ± 0.469
3.94 ± 0.414
6.383 ± 0.574
2.6 ± 0.297
5.713 ± 0.376
7.329 ± 0.806
8.274 ± 1.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.97 ± 0.297
5.201 ± 0.677
5.162 ± 0.722
3.822 ± 0.39
5.201 ± 0.517
6.777 ± 0.299
6.501 ± 0.391
6.541 ± 0.954
1.655 ± 0.253
3.507 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here