Trichechus manatus papillomavirus 4
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6TNL2|A0A0F6TNL2_9PAPI Regulatory protein E2 OS=Trichechus manatus papillomavirus 4 OX=1435497 GN=E2 PE=3 SV=1
MM1 pKa = 7.36 HH2 pKa = 7.39 GVTATLQDD10 pKa = 3.3 IVLYY14 pKa = 9.36 EE15 pKa = 4.17 YY16 pKa = 10.11 PEE18 pKa = 3.81 PHH20 pKa = 6.8 RR21 pKa = 11.84 PVDD24 pKa = 3.51 LHH26 pKa = 7.83 CDD28 pKa = 3.47 EE29 pKa = 4.8 EE30 pKa = 4.69 VAYY33 pKa = 10.98 SDD35 pKa = 4.3 LDD37 pKa = 3.71 EE38 pKa = 5.01 EE39 pKa = 4.61 EE40 pKa = 4.63 EE41 pKa = 4.57 SVSVLQTPYY50 pKa = 10.56 RR51 pKa = 11.84 VLVEE55 pKa = 4.44 CGFCRR60 pKa = 11.84 RR61 pKa = 11.84 PVRR64 pKa = 11.84 IVVVSTGGGIHH75 pKa = 7.3 RR76 pKa = 11.84 LHH78 pKa = 6.56 QLLLEE83 pKa = 4.25 NLQLICPVCARR94 pKa = 11.84 EE95 pKa = 3.89 RR96 pKa = 11.84 TLL98 pKa = 4.45
Molecular weight: 11.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.68
IPC2_protein 4.927
IPC_protein 4.813
Toseland 4.749
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.635
Rodwell 4.711
Grimsley 4.673
Solomon 4.787
Lehninger 4.736
Nozaki 4.914
DTASelect 5.016
Thurlkill 4.749
EMBOSS 4.685
Sillero 4.991
Patrickios 2.053
IPC_peptide 4.8
IPC2_peptide 4.991
IPC2.peptide.svr19 4.889
Protein with the highest isoelectric point:
>tr|A0A0F6TNI9|A0A0F6TNI9_9PAPI Protein E7 OS=Trichechus manatus papillomavirus 4 OX=1435497 GN=E7 PE=3 SV=1
MM1 pKa = 7.33 IVVDD5 pKa = 3.85 NWCLHH10 pKa = 3.99 VKK12 pKa = 10.13 YY13 pKa = 9.69 RR14 pKa = 11.84 DD15 pKa = 3.32 RR16 pKa = 11.84 VRR18 pKa = 11.84 SPKK21 pKa = 9.17 TSYY24 pKa = 9.8 IRR26 pKa = 11.84 RR27 pKa = 11.84 SGRR30 pKa = 11.84 SPLVGMDD37 pKa = 3.61 QPTTLHH43 pKa = 7.21 DD44 pKa = 3.95 LQDD47 pKa = 4.2 YY48 pKa = 10.84 YY49 pKa = 11.72 GLNYY53 pKa = 9.55 WSIEE57 pKa = 4.07 IPCLICKK64 pKa = 10.22 QITTCRR70 pKa = 11.84 DD71 pKa = 3.38 RR72 pKa = 11.84 IHH74 pKa = 7.01 FEE76 pKa = 3.57 RR77 pKa = 11.84 GRR79 pKa = 11.84 FKK81 pKa = 11.22 LKK83 pKa = 9.39 WKK85 pKa = 10.37 CGLPYY90 pKa = 10.23 AICLSCLRR98 pKa = 11.84 FYY100 pKa = 10.66 ATYY103 pKa = 10.38 EE104 pKa = 3.49 RR105 pKa = 11.84 LYY107 pKa = 10.18 QYY109 pKa = 10.7 RR110 pKa = 11.84 YY111 pKa = 8.37 SQPVNDD117 pKa = 4.02 VLQDD121 pKa = 3.24 TGLNLFDD128 pKa = 4.67 LSVRR132 pKa = 11.84 CGLCLALLTFEE143 pKa = 4.97 EE144 pKa = 4.78 KK145 pKa = 10.91 LFLSRR150 pKa = 11.84 TGSWLQLCKK159 pKa = 10.82 DD160 pKa = 3.39 KK161 pKa = 10.99 WRR163 pKa = 11.84 GRR165 pKa = 11.84 CSEE168 pKa = 4.06 CRR170 pKa = 11.84 YY171 pKa = 9.73 HH172 pKa = 7.37
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.516
IPC2_protein 8.331
IPC_protein 8.2
Toseland 7.819
ProMoST 8.682
Dawson 8.843
Bjellqvist 9.18
Wikipedia 8.814
Rodwell 8.873
Grimsley 7.717
Solomon 8.946
Lehninger 8.946
Nozaki 9.341
DTASelect 8.902
Thurlkill 8.931
EMBOSS 9.019
Sillero 9.253
Patrickios 3.694
IPC_peptide 8.931
IPC2_peptide 8.565
IPC2.peptide.svr19 8.576
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2438
98
628
406.3
45.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.44 ± 0.824
2.051 ± 0.782
6.809 ± 0.432
4.963 ± 0.473
3.445 ± 0.523
7.301 ± 1.056
2.297 ± 0.301
4.307 ± 0.792
3.568 ± 0.79
9.393 ± 1.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.518 ± 0.257
3.486 ± 0.479
7.178 ± 1.07
4.225 ± 0.435
7.219 ± 0.545
7.752 ± 0.515
7.301 ± 0.908
5.742 ± 0.474
1.559 ± 0.386
3.445 ± 0.691
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here