Flavonifractor sp. An306

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Flavonifractor; unclassified Flavonifractor

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3799 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4BDB9|A0A1Y4BDB9_9FIRM Uncharacterized protein OS=Flavonifractor sp. An306 OX=1965629 GN=B5F88_17705 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.1KK3 pKa = 10.33LNKK6 pKa = 9.61ILSLTAAAVLTLSLSACSGGTQGNAEE32 pKa = 4.36TPTGSSNTPSAAGDD46 pKa = 4.32GYY48 pKa = 10.61RR49 pKa = 11.84VAVVRR54 pKa = 11.84QLDD57 pKa = 3.82HH58 pKa = 7.97ASMNEE63 pKa = 3.32IRR65 pKa = 11.84DD66 pKa = 4.15AITAEE71 pKa = 4.22LDD73 pKa = 3.19AKK75 pKa = 10.65AAEE78 pKa = 4.4LGISITYY85 pKa = 10.47KK86 pKa = 10.78DD87 pKa = 3.92FNGNNDD93 pKa = 3.84PSTLSQIGAQIIADD107 pKa = 4.47GYY109 pKa = 10.86DD110 pKa = 3.45AIIPIGTTAAQQMVAAAQEE129 pKa = 4.41TEE131 pKa = 4.08TPVIYY136 pKa = 8.89GTVSYY141 pKa = 10.61PEE143 pKa = 4.03AAGLTGISYY152 pKa = 7.85VTGTSDD158 pKa = 4.4ALDD161 pKa = 3.66TEE163 pKa = 4.78FLLDD167 pKa = 4.46MMLAQNPDD175 pKa = 3.34VEE177 pKa = 4.9TVGLLYY183 pKa = 10.1STSEE187 pKa = 4.3VNSGPAIEE195 pKa = 4.45AAKK198 pKa = 10.52SYY200 pKa = 11.22LDD202 pKa = 3.65GKK204 pKa = 10.03GISYY208 pKa = 10.14IDD210 pKa = 3.37KK211 pKa = 10.06TGNTTDD217 pKa = 4.05EE218 pKa = 3.85IVAAVNSMLDD228 pKa = 3.12QVDD231 pKa = 4.08AVFTPTDD238 pKa = 3.56NVVMNAEE245 pKa = 4.1LAIAPTLAEE254 pKa = 4.33AGIPHH259 pKa = 6.57YY260 pKa = 10.82AGADD264 pKa = 3.38SFVRR268 pKa = 11.84NGAFATCGVNYY279 pKa = 9.67TDD281 pKa = 6.11LGTQTADD288 pKa = 3.27LALEE292 pKa = 4.1VLQTGEE298 pKa = 3.93VPEE301 pKa = 4.45FVTVAGDD308 pKa = 3.99IITVNTEE315 pKa = 3.32TAAALGVDD323 pKa = 3.32YY324 pKa = 11.23AVFNDD329 pKa = 3.76MGSSLVEE336 pKa = 3.83VQTTQEE342 pKa = 3.66

Molecular weight: 35.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4BXX0|A0A1Y4BXX0_9FIRM TIGR03905 family protein OS=Flavonifractor sp. An306 OX=1965629 GN=B5F88_09465 PE=4 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84TYY5 pKa = 10.22QPKK8 pKa = 9.24KK9 pKa = 7.68RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.13GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MRR23 pKa = 11.84TANGRR28 pKa = 11.84KK29 pKa = 8.55VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.28

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3799

0

3799

1118621

30

2357

294.5

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.5 ± 0.052

1.598 ± 0.019

5.515 ± 0.035

6.942 ± 0.042

3.683 ± 0.028

7.932 ± 0.044

1.847 ± 0.018

5.24 ± 0.036

4.588 ± 0.04

10.28 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.02

3.209 ± 0.029

4.374 ± 0.026

3.935 ± 0.028

5.824 ± 0.045

5.409 ± 0.036

5.613 ± 0.05

7.121 ± 0.037

1.176 ± 0.017

3.518 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski